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Các bài báo trên các tạp chí thuộc danh mục isi/scopus năm học 2019-2020

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1

Phuong Le Thi, Yunki Lee, Dieu Linh Tran, Thai Thanh Hoang Thi, Jeon Il Kang, Kyung Min Park, Ki Dong Park, “In situ forming and reactive oxygen species-scavenging gelatin hydrogels for enhancing wound healing efficacy.” Acta biomaterialia vol. 103 (2020): 142-152 (ISI, IF: 6.319)

https://doi.org/10.1016/j.actbio.2019.12.009

Abstract:

Lymphoid organs, which are populated by dendritic cells (DCs), are highly specialized tissues and provide an ideal microenvironment for T-cell priming. However, intramuscular or subcutaneous delivery of vaccine to DCs, a subset of antigen-presenting cells, has failed to stimulate optimal immune response for effective vaccination and need for adjuvants to induce immune response. To address this issue, we developed an in situ-forming injectable hybrid hydrogel that spontaneously assemble into microporous network upon subcutaneous administration, which provide a cellular niche to host immune cells, including DCs. In situ-forming injectable hybrid hydrogelators, composed of protein-polymer conjugates, formed a hydrogel depot at the close proximity to the dermis, resulting in a rapid migration of immune cells to the hydrogel boundary and infiltration to the microporous network. The biocompatibility of the watery microporous network allows recruitment of DCs without a DC enhancement factor, which was significantly higher than that of traditional hydrogel releasing chemoattractants, granulocyte macrophage colony-stimulating factor. Owing to the sustained degradation of microporous hydrogel network, DNA vaccine release can be sustained, and the recruitment of DCs and their homing to lymph node can be modulated. Furthermore, immunization of a vaccine encoding amyloid-β fusion proteinbearing microporous network induced a robust antigen-specific immune response in vivo and strong recall immune response was exhibited due to immunogenic memory. These hybrid hydrogels can be administered in a minimally invasive manner using hypodermic needle, bypassing the need for cytokine or DC enhancement factor and provide niche to host immune cells. These findings highlight the potential of hybrid hydrogels that may serve as a simple, yet multifunctional, platform for DNA vaccine delivery to modulate immune response.

 

2

Phuong Le Thi, Yunki Lee, Dieu Linh Tran, Thai Thanh Hoang Thi, Jeon Il Kang, Kyung Min Park, Ki Dong Park, “In situ forming and reactive oxygen species-scavenging gelatin hydrogels for enhancing wound healing efficacy.” Acta biomaterialia vol. 103 (2020): 142-152 (ISI, IF: 6.319)

https://doi.org/10.1016/j.actbio.2019.12.009

Abstract:

The overexpression of reactive oxygen species (ROS) contributes to the pathogenesis of numerous diseases such as atherosclerosis, myocardial infarction, cancer, and chronic inflammation. Therefore, the development of materials that can locally control the adverse effects resulting from excessive ROS generation is of great significance. In this study, the antioxidant gallic acid-conjugated gelatin (GGA) was introduced into gelatin-hydroxyphenyl propionic (GH) hydrogels to create an injectable hydrogel with enhanced free radical scavenging properties compared to pure GH hydrogels. The modified hydrogels were rapidly formed by an HRP-catalyzed cross-linking reaction with high mechanical strength and biodegradability. The resulting GH/GGA hydrogels effectively scavenged the hydroxyl radicals and DPPH radicals, and the scavenging capacity could be modulated by varying GGA concentrations. Moreover, in an in vitro H2O2-induced ROS microenvironment, GH/GGA hydrogels significantly suppressed the oxidative damage of human dermal fibroblast (hDFBs) and preserved their viability by reducing intracellular ROS production. More importantly, the ROS scavenging hydrogel efficiently accelerated the wound healing process with unexpected regenerative healing characteristics, shown by hair follicle formation; promoted neovascularization; and highly ordered the alignment of collagen fiber in a full-thickness skin defect model. Therefore, we expect that injectable GH/GGA hydrogels can serve as promising biomaterials for tissue regeneration applications, including wound treatment and other tissue repair related to ROS overexpression. STATEMENT OF SIGNIFICANCE: Recently, many researchers have endeavored to develop injectable hydrogel matrices that can modulate the ROS level to normal physiological processes for the treatment of various diseases. Here, we designed an injectable gelatin hydrogel in which gallic acid, an antioxidant compound, was conjugated onto a gelatin polymer backbone. The hydrogels showed tunable properties and could scavenge the free radicals in a controllable manner. Because of the ROS scavenging properties, the hydrogels protected the cells from the oxidative damage of ROS microenvironment and effectively accelerated the wound healing process with high quality of healed skin. We believe that this injectable ROS scavenging hydrogel has great potential for wound treatment and tissue regeneration, where oxidative damage by ROS contributes to the pathogenesis.

3

Quoc Hanh Nguyen; Quoc Hai Nguyen; Hur, Jaehyun, "High-performance ZnTe-TiO2-C nanocomposite with half-cell and full-cell applications as promising anode material for Li-Ion batteries", Applied Surface Science 509 (2020) 144718 (ISI, IF: 6.182).

https://www.sciencedirect.com/science/article/abs/pii/S0169433219335342

Abstract:

C-coated ZnTe-TiO2 nanocomposite is successfully synthesized via annealing followed by high-energy mechanical milling. ZnTe-TiO2-C nanocomposite is comprised of intermetallic ZnTe nanoalloy homogeneously distributed in a hybrid matrix of rutile TiO2 and conductive amorphous C. The presence of rutie TiO2 and C significantly improves the electrochemical performance of ZnTe; that is, highly stable cyclic performance (611 mAh g−1 at 0.1 A g−1 with ~95% capacity retention over 300 cycles and 492 mAh g−1 at 0.5 A g−1 after 500 cycles) and good rate capability (76% capacity retention at 10 A g−1 compared with the capacity at 0.1 A g−1). The improved electrochemical performance is primarily associated with the TiO2-C hybrid matrix that efficiently surrounds the ZnTe nanocrystallites, thereby providing high electric conductivity and at the same time functioning as a buffering medium that alleviates the large volume change during extended cycling. Furthemore, the practical application of ZnTe-TiO2-C as an anode is investigated with full cell consisting of a ZnTe-TiO2-C anode and a LiFePO4-graphite (LFP@G) cathode. The ZnTe-TiO2-C//LFP@G full cell exhibits good cyclic stability with 79% capacity retention at 0.1 A g−1 after 200 cycles. These results suggest the ZnTe-TiO2-C nanocomposite is a promising candidate for a high-performance anode in next-generation LIBs.

 

Nguyen QuangKhuyen; Jose G.Martinez; FriedrichKaasik; TarmoTamm; Toribio F.Otero; RudolfKiefer, “Solvent effects on carbide-derived-carbon trilayer bending actuators”, Synthetic Metals 247 (2019), 170-176, (ISI, IF: 3.286).

https://www.sciencedirect.com/science/article/abs/pii/S0379677918305861

Abstract:

Bending actuators were prepared by depositing carbide-derived carbon, a material typical for electric double layer capacitors, on both sides of poly-vinylidenefluoride membranes, forming CDC-trilayers. Their actuation properties were studied using 0.5 M solutions of lithium perchlorate (LiClO4) in different solvents: water, ethylene glycol, acetonitrile, and propylene carbonate. The goal of this work was to study the actuation mechanism, charging-discharging properties in these solvents, as well as to establish the optimal solvent for maximum bending displacement. It was found that while the actuation direction was the same for all solvents, pointing to similar mechanism, the exchanged charge and the displacement differed considerably. Moreover, the highest specific capacitance found in ethylene glycol did not bring along the highest displacement, neither was the highest exchanged charge of propylene carbonate the most efficient option, the acetonitrile was the clear winner. The available electrochemical windows for the reversible charging also differed considerably.

5

Tran Thien Khanh, Rong-Jer Lee, Paul A. Kilmartin, Md. Asaduzzaman Khan, Mahdi Safaei Khorram, Tarmo Tamm, Rudolf Kiefer, "Actuation increase in polypyrrole bilayer by photo-activated dopants", Synthetic Metals 246 (2018), 57-63, (ISI, IF 3.286)

https://doi.org/10.1016/j.synthmet.2018.10.001.

Abstract:

A new methodology to increase the polypyrrole (PPy) bilayer actuation displacement is presented, based on photo-activated dopants generating secondary charges. Two dopants, dodecyl benzenesulfonate (DBS) and the photo-active dopant 2-diazo-1-naphthol-5-sulfonic acid (DNSA), were compared in this study. PPy/DBS, PPy/DBS-DNSA and PPy/DNSA bilayers on polyethylene terephthalate were formed and their actuation properties in aqueous electrolyte were investigated applying cyclic voltammetry and square wave potential steps. Exposure to solar irradiation increased PPy/DBS-DNSA and PPy/DNSA bilayer bending displacements by two and three times, respectively, accompanied by increased charge density during the reversible redox cycles. UV–vis and Fourier transform infrared (FTIR) measurements were also performed to follow the photo reaction of the photo-active dopants.

6

Tran Thien Khanh, Arko Kesküla, Zane Zondaka, Madis Harjo, Alo Kivilo, Mahdi Safaei Khorram, Tarmo Tamm, Rudolf Kiefer, “Role of polymerization temperature on the performance of polypyrrole/dodecyl benzenesulphonate linear actuator”, Synthetic Metals, 247, (2019), 53-58, ISI, IF 3.28 6

https://doi.org/10.1016/j.synthmet.2018.11.013.

Abstract:

Polypyrrole doped with dodecylbenzenesulphonate (PPy/DBS) free-standing films were electropolymerized in sodium perchlorate propylene carbonate solution at different temperatures, observing increasing conductivity with decreasing synthesis temperature. Our goal in this study was to evaluate how the linear actuation of PPy depends on the polymerization temperature. The anion driven actuation of materials synthesized at lower temperatures changed to mainly cation active for PPy/DBS films polymerized at 20 °C; the latter films also showed the highest diffusion coefficients. Scanning electron microscopy did show that the surface roughness of the films increased with increasing synthesis temperature, as expected. Isometric and isotonic electro-chemo mechanical deformation (ECMD) measurements were performed with combined electrochemical techniques (cyclic voltammetry and chronoamperometry), revealing wide differences in the actuation behavior.

7

"Carbide-derived carbon and poly-3,4-ethylenedioxythiphene composite laminate: linear and bending actuation", Synthetic Metals 245 (2018) 67-73 (ISI, IF: 3.286)

https://www.sciencedirect.com/science/article/abs/pii/S0379677918302443

Abstract:

Carbide-derived carbon is a well-known material for ionic electroactive systems like sensors and actuators. Typically, a gold foil or other current collector is applied on top of either side to enhance the conductivity of such laminates. Here, metal foil is replaced by an electrochemically deposited layer of poly-3,4-ethylenedioxythiophene (PEDOT). While lower in conductivity, PEDOT has the advantages of lowered risk of delamination and being electro-chemo-mechanically active itself. Such laminates were tested in both linear actuation mode in lithium bis(trifluoromethane) sulfonamide propylene carbonate solution (LiTFSI-PC) under isotonic and isometric electro-chemo-mechanical deformation measurements (ECMD), and in bending mode in air using a room-temperature ionic liquid as the electrolyte. The exchanged charge as well as the actuation response of the laminate was dominated by the PEDOT layer, overriding even the actuation direction of the carbon-based layers. The stress of all investigated samples was found in similar range of 30 kPa. The materials were characterized by scanning electron microscopy (SEM), FTIR and energy-dispersive X-Ray spectroscopy (EDX).

8

Minh Nhat Ho, Long Giang Bach, Dai Hai Nguyen, Cong Hao Nguyen, Cuu Khoa Nguyen, Ngoc Quyen Tran, Ngoc Vinh Nguyen, Thai Thanh Hoang Thi, “PEGylated PAMAM dendrimers loading oxaliplatin with prolonged release and high payload without burst effect”, BIOPOLYMERS, 2019, DOI: 10.1002/bip.23272 (ISI, IF: 2.248)

Abstract:
Oxaliplatin (OXA) was coupled to PEGylated polyamidoamine dendrimers of fourth generation (G4‐PEG@OXA) in the comparison to PEGylated ones of odd generation (G3.5‐PEG@OXA). Proton nuclear magnetic resonance and Fourier‐transform infrared spectroscopy were used to confirm the successful incorporation of OXA as well as the synthesis of carrier systems. Both two types of carrier systems exhibited in sphere nanoparticle shape with size of less than 100 nm that was in the range being able to cause toxicity on cancer cells. The average drug loading efficiency (DLE) of G4‐PEG@OXA was obtained at 84.63% that was higher than DLE of G3.5‐PEG of 75.69%. The release kinetic of G4‐PEG@OXA and G3.5‐PEG@OXA did not show any burst release phenomenon while free OXA was released over 40% at the first hour. The sustainable release of OXA was achieved when it was encapsulated in these carriers, but the G4 generation liberated OXA (3.4%‐6.4%) slower than G3.5 one (11.9%‐22.8%). The in vitro cytotoxicities of G4‐PEG@OXA were evaluated in HeLa cell lines using resazurin assay and live/dead staining test. Although the free OXA showed a rather moderate killing ability, the G4‐PEG@OXA still displayed the low viability of HeLa that was better to the result of G3.5‐PEG@OXA due to released OXA amount. The benefit of this system was to overcome the burst release phenomenon to minimize OXA toxicity without compromising its efficiency..
9

Removal of various contaminants from water by renewable lignocellulose-derived biosorbents: a comprehensive and critical review

Abstract:

Contaminants in water bodies cause potential health risks for humans and great environmental threats. Therefore, the devel- opment and exploration of low-cost, promising adsorbents to remove contaminants from water resources as a sustainable option is one focus of the scientific community. Here, we con- ducted a critical review regarding the application of pristine and modified/treated biosorbents derived from leaves for the removal of various contaminants. These include potentially toxic cationic and oxyanionic metal ions, radioactive metal ions, rare earth elements, organic cationic and anionic dyes, phosphate, ammonium, and fluoride from water media. Similar to lignocellulose-based biosorbents, leaf-based biosorbents exhibit a low specific surface area and total pore volume but have abundant surface functional groups, high concentrations of light metals, and a high net surface charge density. The maximum adsorption capacity of biosorbents strongly depends on the operation conditions, experiment types, and adsorbate nature. The absorption mechanism of contaminants onto biosorbents is complex; therefore, typical experiments used to identify the primary mechanism of the adsorption of contaminants onto biosorbents were thoroughly discussed. It was concluded that byproduct leaves are renew- able, biodegradable, and promising biosorbents which have the potential to be used as a low-cost green alternative to commer- cial activated carbon for effective removal of various contami- nants from the water environment in the real-scale plants.

 

10

Balamurugan Shanmugaraj, Lohanathan Bharathi Priya, B. Mahalakshmi, Shanmugam Subbiah, Rouh-Mei Hu, Bharath Kumar Velmurugan, Rathinasamy Baskaran, "Bacterial and viral vectors as vaccine delivery vehicles for breast cancer therapy", Life Sciences 250 (2020) 117550 (ISI, IF: 3.647)

https://www.sciencedirect.com/science/article/abs/pii/S0024320520302988

Abstract:

Breast cancer is the frequently diagnosed cancer among women and it is the most lethal malignancy in women globally. With one million cases every year, breast cancer is the fast-growing cancer type that has a high prevalence rate in young women. The limitations and undesirable side effects of conventional therapies like chemotherapy and radiotherapy on malignant tumors necessitate the development of alternative therapeutic approaches. Gene therapy has emerged as a promising approach to cure a variety of malignant cancer types which involves the delivery of functional gene directly into the target tumor tissue. Efficient gene therapy approach relies on the effective delivery of therapeutic genes to the desired cell type. In this regard, biological and non-biological gene delivery vectors are used to protect the naked foreign DNA to mediate effective tissue entry of the desired gene of interest. In this review, the use of bacterial and viral vectors for breast cancer gene therapy was summarized.

 

11

Dieu Linh Tran, Phuong Le Thi, Thai Thanh Hoang Thi, Ki Dong Park, “Graphene Oxide Immobilized Surfaces Facilitate the Sustained Release of Doxycycline for the Prevention of Implant Related Infection”, COLLOIDS AND SURFACES B: BIOINTERFACES, 2019, 181, 576. (ISI, IF: 3.997)

https://www.sciencedirect.com/science/article/abs/pii/S0927776519304072

Abstract:

Preventing implant-associated infection, which can lead to implant failure and increased medical costs, is one of the biggest challenges in the orthopaedic surgeons. Therefore, the development of stable and highly effective surface modifications to increase the antimicrobial properties of implants is required. In this study, graphene oxide (GO-)-immobilized titanium dioxide (TiO₂) was developed to efficiently carry and release antimicrobial drugs. Firstly, tyramine-conjugated GO (GOTA) was synthesized and immobilized onto the surfaces of TiO₂ through tyrosinase (Tyr)-catalyzed oxidative reaction (GOTA/TiO₂). Doxycycline hyclate (Dox) was then loaded onto GOTA/TiO₂ via non-covalent interactions between GO and Dox (Dox/GOTA/TiO₂), including electrostatic interaction, π-π stacking, hydrophobic interaction, and hydrogen bonds. The amount of loaded drug was able to be controlled, reaching a maximum of 36 μg/cm2. in vitro experiments revealed that the sustained release of Dox from the TiO₂ surfaces continued for over 30 days. Compared with bare TiO₂ and GOTA/TiO2, Dox/GOTA/TiO₂ exhibited superior antibacterial activity against both gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria, without affecting the viability of human dermal fibroblasts. The obtained results indicated that GO-immobilized TiO₂ is an effective carrier for antimicrobial drug delivery to reduce implant-associated infection through the synergistic antimicrobial effect of GO and the prescribed drugs.

12

Hoang Chinh Nguyen, My Linh Nguyen, Fu-Ming Wang, Horng-Yi Juan, Chia-Hung Su, "Biodiesel production by direct transesterification of wet spent coffee grounds using switchable solvent as a catalyst and solvent", Bioresource Technology, 296, (2020), 122334 (ISI, IF: 7.539)

https://www.sciencedirect.com/science/article/abs/pii/S0960852419315640

Abstract:

Spent coffee grounds (SCGs) are a promising material for sustainable preparation of biodiesel. This study pro- posed a new approach for biodiesel synthesis from wet SCGs using 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) as both a green solvent and catalyst. The optimal reaction conditions were determined as a methanol amount of 6.25 mL/g of wet SCGs, DBU amount of 14.46 mL/g of wet SCGs, temperature of 60.2 °C, and reaction time of 28.65 min through response surface methodology. Under these conditions, the maximum biodiesel yield was 97.18%. Notably, DBU polarity could be regulated reversibly, facilitating its reusability and a simple process for product separation. Under optimal conditions, DBU could be potentially reused for at least 10 cycles to yield high amounts of biodiesel. This study suggests that the switchable solvent-assisted direct transesterification of wet SCGs is a potential, efficient, cost-effective, and eco-friendly approach for biodiesel synthesis.

 

13

 

Hao Huy Nguyen, Gobinda Gyawali, Adriana Martinez-Oviedo, Yuwaraj K. Kshetri, Soo Wohn Lee, "Physicochemical properties of Cr-doped TiO2 nanotubes and their application in dye-sensitized solar cells", Journal of Photochemistry & Photobiology A: Chemistry, (2020) (ISI, IF 3.261)

https://www.sciencedirect.com/science/article/abs/pii/S1010603020303130

 

Abstract:
Doping is always known as an effective way to modify the properties of a semiconductor. In this work, Cr-doped TiO2 nanotubes (Cr-TNTs) with different Cr contents were synthesized via a microwave-assisted hydrothermal method. The changes in crystal structures, chemical and electronic states, and the band gap structure of the obtained Cr-doped TiO2 nanomaterials were analyzed, thereby confirming the effect of the doping on physicochemical properties of the products. Moreover, the existence of Ti3+ sites and oxygen vacancies have significantly influenced the separation of electron-hole pairs as predicted by the photoluminescence spectra. The EIS results indicated that Cr-doping on TNT made a significant contribution to reducing the recombination rate of photogenerated charge carriers, leading to a prolonged lifetime of photogenerated electrons. The Cr-TNT based dye-sensitized solar cell (DSSC) containing 7.5 atomic percentage of Cr/Ti has demonstrated the best efficiency on the solar light conversion among the prepared samples.
14

A sequence-based approach for identifying recombination spots in Saccharomyces cerevisiae by using hyper-parameter optimization in FastText and support vector machine

Abstract:

Meiotic recombination is a biological process which plays a crucial role in genetic evolution. Therefore, the ability of machine learning models in extracting desire information embedded in DNA sequences has drawn a great deal of attention among biologists. Recently, several attempts have been made to address this problem, however, the performance results still need to be improved. The current study aims to investigate the relationship between natural language processing model and supervised learning in classifying DNA sequences. The idea is to treat DNA sequences by FastText model, including sub-word information and then use them as features in a suitable supervised learning algorithm. To the end, this hybrid approach helps us classify DNA recombination spots with achieved sensitivity of 90%, specificity of 94.76%, accuracy of 92.6%, and MCC of 0.851. These results have suggested that our newly proposed method is superior to other methods on the same benchmark dataset. This study, therefore, could shed the light on developing the prediction models for recombination spots in particular, and DNA sequences in general.

15

Thai Thanh Hoang Thi, Van Du Cao, Thi Nhu Quynh Nguyen, Duc Thuan Hoang, Van Cuong Ngo, Dai Hai Nguyen, “Functionalized mesoporous silica nanoparticles and biomedical applications”, MATERIALS SCIENCE AND ENGINEERING: C, 2019, 99, 631.(ISI, IF: 5.88)

https://www.sciencedirect.com/science/article/abs/pii/S0928493117343461

Abstract:

Since the first report in early 1990s, mesoporous silica nanoparticles (MSNs) have progressively attracted the attention of scientists due to their potential applications in physic, energy storage, imaging, and especially in biomedical engineering. Owning the unique physiochemical properties, such as highly porosity, large surface area and pore volume, functionalizable, tunable pore and particle sizes and biocompatibility, and high loading cavity, MSNs offer efficient encapsulation and then controlled release, and in some cases, intracellular delivery of bioactive molecules for biomedical applications. During the last decade, functionalized MSNs that show respond upon the surrounding stimulus changes, such as temperature, pH, redox, light, ultrasound, magnetic or electric fields, enzyme, redox, ROS, glucose, and ATP, or their combinations, have continuously revolutionized their potential applications in biomedical engineering. Therefore, this review focuses on discussion the recent fabrication of functionalized MSNs and their potential applications in drug delivery, therapeutic treatments, diagnostic imaging, and biocatalyst. In addition, some potential clinical applications and challenges will also be discussed.

16

Minh Nhat Ho, Long Giang Bach, Thi Hiep Nguyen, Minh Hieu Ho, Dai Hai Nguyen, Cuu Khoa Nguyen, Cong Hao Nguyen, Ngoc Vinh Nguyen, Thai Thanh Hoang Thi, “PEGylated poly(amidoamine) dendrimers-based drug loading vehicles for delivering carboplatin in treatment of various cancerous cells”, JOURNAL OF NANOPARTICLE RESEARCH, 2019, 21, 43. (ISI, IF: 2.009)

https://link.springer.com/article/10.1007/s11051-019-4486-5

Abstract:
The fourth generation (G4) of poly(amidoamine) dendrimer was PEGylated and then physically incorporated with carboplatin to develop the new drug delivery carrier. The proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR) were utilized to confirm the successful synthesis and incorporation. The PEGylation leads to increase the particle dimension of G4 dendrimer to 4.04 to 30.67 nm that was still in the effective range for killing cancerous cells. The cytotoxicity on L929 fibroblasts demonstrated that the PEGylation of G4 could eliminate the toxic caused by amine surrounding groups of G4. Three cancerous cells including the cervical cancer cell line (HeLa), the adenocarcinomic human alveolar basal epithelial cells (A549), and the human breast cancer cell line (MCF7) were treated with G4-PEG@CAR as a function of CAR concentrations. The results of resazurin test and live/dead assay indicated that the killing effect of G4-PEG@CAR on three representative cells were achieved and increased following the enhancement of CAR concentration. Furthermore, the better effectiveness of G4-PEG@CAR was achieved on Hela.
17

Minh Thanh Vu, Long Giang Bach, Duy Chinh Nguyen, Minh Nhat Ho, Ngoc Hoi Nguyen, Ngoc Quyen Tran, Dai Hai Nguyen, Cuu Khoa Nguyen, Thai Thanh Hoang Thi, “Modified Carboxyl-Terminated PAMAM Dendrimers as Great Cytocompatible Nano-Based Drug Delivery System”, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20(8), 2016. (ISI, IF: 4.556)

https://www.mdpi.com/1422-0067/20/8/2016

Abstract:
Polyamidoamine (PAMAM) dendrimers are extensively researched as potential drug delivery system thanks to their desirable features such as controlled and stable structures, and ease of functionalization onto their surface active groups. However, there have been concerns about the toxicity of full generation dendrimers and risks of premature clearance from circulation, along with other physical drawbacks presented in previous formulations, including large particle sizes and low drug loading efficiency. In our study, carboxyl-terminated PAMAM dendrimer G3.5 was grafted with poly (ethylene glycol) methyl ether (mPEG) to be employed as a nano-based drug delivery system with great cytocompatibility for the delivery of carboplatin (CPT), a widely prescribed anticancer drug with strong side effects so that the drug will be effectively entrapped and not exhibit uncontrolled outflow from the open structure of unmodified PAMAM G3.5. The particles formed were spherical in shape and had the optimal size range (around 36 nm) that accommodates high drug entrapment efficiency. Surface charge was also determined to be almost neutral and the system was cytocompatible. In vitro release patterns over 24 h showed a prolonged CPT release compared to free drug, which correlated to the cytotoxicity assay on malignant cell lines showing the lack of anticancer effect of CPT/mPEG-G3.5 compared with CPT.
18

Ngoc Thuy Trang Le, Van Du Cao, Thi Nhu Quynh Nguyen, Thi Thu Hong Le, Thach Thao Tran, Thai Thanh Hoang Thi, “Soy Lecithin-Derived Liposomal Delivery Systems: Surface Modification and Current Applications”, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20(19), 4706. (ISI, IF: 4.556)

https://www.mdpi.com/1422-0067/20/19/4706

Abstract:
The development of natural phospholipids for nanostructured drug delivery systems has attracted much attention in the past decades. Lecithin that was derived from naturally occurring in soybeans (SL) has introduced some auspicious accomplishments to the drug carrying aspect, like effectual encapsulation, controlled release, and successful delivery of the curative factors to intracellular regions in which they procure these properties from their flexible physicochemical and biophysical properties, such as large aqueous center and biocompatible lipid, self-assembly, tunable properties, and high loading capacity. Despite the almost perfect properties as a drug carrier, liposome is known to be quite quickly eliminated from the body systems. The surface modification of liposomes has been investigated in many studies to overcome this drawback. In this review, we intensively discussed the surface-modified liposomes that enhancing the targeting, cellular uptake, and therapeutic response. Moreover, the recent applications of soy lecithin-derived liposome, focusing on cancer treatment, brain targeting, and vaccinology, are also summarized.
19

Using extreme gradient boosting to identify origin of replication in Saccharomyces cerevisiae via hybrid features

Abstract:
DNA replication is a fundamental task that plays a crucial role in the propagation of all living things on earth. Hence, the accurate identification of its origin could be the key to giving an insightful understanding of the regulatory mechanism of gene expression. Indeed, with the robust development of computational techniques and the abundant biological sequencing data, it has become possible for scientists to identify the origin of replication accurately and promptly. This growing concern has drawn a lot of attention among experts in this field. However, to gain better outcomes, more work is required. Therefore, this study is designed to explore the combination of state-of-the-art features and extreme gradient boosting learning system in classifying DNA sequences. Our hybrid approach is able to identify the origin of DNA replication with achieved sensitivity of 85.19%, specificity of 93.83%, accuracy of 89.51%, and MCC of 0.7931. Evidence is presented to show that our proposed method is superior to the state-of-the-art methods on the same benchmark dataset. Moreover, the research results represent a further step towards developing the prediction models for DNA replication in particular and DNA sequences in general.
20

Hoang Chinh Nguyen, Hwai Chyuan Ong, Thi Thanh Truc Pham, Thi Kim Khue Dinh, and Chia‐Hung Su, "Microwave‐mediated noncatalytic synthesis of ethyl levulinate: A green process for fuel additive production", International Journal of Energy Research, 44, 3 (2020), 1698-1708 (ISI, IF: 3.741)

https://onlinelibrary.wiley.com/doi/abs/10.1002/er.4985

Abstract:

This study developed a new catalyst-free process for producing ethyl levulinate, a biofuel additive. Noncatalytic levulinic acid esterification with ethanol using microwave irradiation (MW) was compared with that using traditional heating (TH) under different reaction conditions. The results demonstrated that the esterification process using MW was more effective than that using TH. A reaction conversion of 90.38% was obtained for the esterification using MW at 473 K and reaction time of 3 hours. Moreover, this study established a model for depicting the kinetics of levulinic acid esterification using MW and TH. A good fit to the data (R2 of >.9999) was achieved, indicating the validity of the developed model. The rate constants and pre-exponential factor obtained for the esterification using MW were greater than those obtained using TH. Consequently, the microwave-assisted noncatalytic synthesis is a green and promising method for preparing ethyl levulinate.

21

Ngoc Thuy Trang Le, Long Giang Bach, Duy Chinh Nguyen, Tran Hong Xuan Le, Khanh Hung Pham, Dai Hai Nguyen, Thai Thanh Hoang Thi, “Evaluation of Factors Affecting Antimicrobial Activity of Bacteriocin from Lactobacillus plantarum Microencapsulated in Alginate-Gelatin Capsules and Its Application on Pork Meat as a Bio-Preservative”, INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2019, 16(6), E1017 (ISI, IF: 2.849)

https://www.mdpi.com/1660-4601/16/6/1017

Abstract:
Antimicrobial compounds from traditional fermented foods have shown activity against a wide range of pathogen and spoilage microorganisms for several years. In this study, a Lactic acid bacteria (LAB), isolated from Vietnamese traditional fermented yogurt (Lactobacillus plantarum SC01), was encapsulated in alginate-gelatin (ALG-GEL) and the effect of incubation temperature, medium pH and surfactants were assessed. The aims of this research were to evaluate antimicrobial activity of bacteriocin produced by L. plantarum SC01. Another aim the research was to study the quality of pork meat treated with its Bacteriocin in 2 h as a bio-preservative at different storage times (0 h, 12 h, 24 h and 48 h) in room temperature, compared to control (treated with salt 40.0%). The antimicrobial activity of L. plantarum SC01 was identified through the inhibition rate of five indicator organisms, including Escherichia coli, Salmonella sp., Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis by co-culture method. The results showed that L. plantarum SC01 microencapsulated in ALG-GEL (2.5% alginate and 6.0% gelatin, w/v) and 3.0% bacteria supplied into modified MRS medium (MRSOPTSC01) produced highly active compound inhibited the growth of indicator organisms at a density of 104–108 CFU/mL. Antibacterial compounds were highly active in a treatment at 80 °C; not to be affected by pH; affected by surfactant as Ethylenediaminetetraacetic acid (EDTA), Sodium dodecyl sulfate (SDS), and Tween. Moreover, LAB obtained from this study show the potent Bacteriocin in its usage as a preservative in food.
22

Vu Minh Thanh, Le Minh Bui, Long Giang Bach, Ngoc Tung Nguyen, Hoa Le Thi, Thai Thanh Hoang Thi, “Origanum majorana L. Essential Oil-Associated Polymeric Nano Dendrimer for Antifungal Activity against Phytophthora infestans”, MATERIALS (Basel), 2019, 12(9), 1446 (ISI, IF: 3.057)

https://www.mdpi.com/1996-1944/12/9/1446

Abstract:
In this study, the introduction of Origanum majorana L. essential oil into a polyamidoamine (PAMAM) G4.0 dendrimer was performed for creation of a potential nanocide against Phytophthora infestans. The characteristics of marjoram oil and PAMAM G4.0 was analyzed using transmission electron spectroscopy (TEM), nuclear magnetic resonance spectroscopy (1H-NMR) and gas chromatography mass spectrometry (GC-MS). The success of combining marjoram oil with PAMAM G4.0 was evaluated by FT-IR, TGA analysis, and the antifungal activity of this system was also investigated. The results showed that the antifungal activity of oil/PAMAM G4.0 was high and significantly higher than only PAMAM G4.0 or marjoram essential oil. These results indicated that the nanocide oil/PAMAM G4.0 helped strengthen and prolong the antifungal properties of the oil.
23

Velmurugan BK, Baskaran R, Huang CY. "Detailed insight on β-adrenoceptors as therapeutic targets" Biomedicine & Pharmacotherapy, 117 (2019) 109039 (ISI, IF: 4.545)

https://doi.org/10.1016/j.biopha.2019.109039

Abstract:
Human G protein-coupled receptors (GPCRs), especially adrenoceptors, play a crucial role in maintaining important physiological activities including cardiovascular and pulmonary functions. Among all adrenoceptors, β-adrenoceptors are the best characterized GPCRs and possess distinctive features as drug targets. Similarly, ligands that activate/deactivate β-adrenoceptors also hold a significant position in the field of biomarker identification and drug discovery. Several studies regarding molecular characterization of the β-adrenoceptor ligands have revealed that ligands with abilities to inhibit basal or intrinsic receptor activity or prevent receptor desensitization are particularly important to efficiently manage detrimental health conditions, including chronic heart failure, asthma, chronic obstructive pulmonary disease, obesity, and diabetes. Given the importance of β-adrenoceptors as molecular targets for many pathological conditions, this review aims to provide a detailed insight on the structural and functional aspects of β-adrenoceptors, with a particular emphasis on their importance as biomarkers and therapeutic targets.
24

 

Seong Han Kim, Thavasyappan Thambi, Jae Seung Lym, V.H. Giang Phan, and Doo Sung Lee, Tunable Engineering of Heparinized Injectable Hydrogels for Affnity-Based Sustained Delivery of Bioactive Factors, Macromol. Mater. Eng. 2019, 1900279 (ISI, IF: 3.038)

https://onlinelibrary.wiley.com/doi/10.1002/mame.201900279

Abstract:
Here, the design of an in situ‐forming injectable hydrogel is reported based on pH‐ and temperature‐responsive copolymers finely engineered with heparin for the sustained delivery of bioactive factors. In order to develop such heparinized injectable hydrogels, pH‐ and temperature‐responsive copolymers based on poly(ethylene glycol) and poly(urethane sulfamethazine) (PEG‐PUSSM) are synthesized and acrylated, and subsequently coupled with thiolated heparin through Michael‐addition reaction. The content of heparin in the bioconjugates (Hep‐PUSSM) is finely tuned to control the release of heparin‐binding bioactive factors. The free‐flowing bioconjugate sols at room temperature transform to stable viscoelastic gel in physiological conditions, indicating that heparin modification does not affect the sol–gel transition. The subcutaneous administration of bioconjugate sols to the dorsal‐region of Sprague‐Dawley rats forms a hydrogel depot and shows controlled degradation. The bioconjugates effectively bind with bioactive factors (VEGF) through simple mixing, and the release is controlled over a period of 4 weeks without an initial burst. As a result, the implantation of VEGF‐loaded bioconjugate gel induces angiogenesis throughout the hydrogel network. The tunable engineering of the injectable hydrogel by heparinization with independent controllable physical properties sustains the release of bioactive factors, indicating that it may be a promising platform for the delivery of bioactive factors.
25

Hoang Chinh Nguyen, My Linh Nguyen, Shih-Hsiang Liang, Chia-Hung Su, Fu-Ming Wang, "Switchable Solvent-Catalyzed Direct Transesterification of Insect Biomass for Biodiesel Production", BioEnergy Research, 13, (2019), 563-570 (ISI, IF: 2.195) https://link.springer.com/article/10.1007/s12155-019-10085-8

Abstract:
DBU (1,8-diazabicyclo[5.4.0]undec-7-ene), a polarity switchable solvent, has been recognized as a green solvent for oil extrac- tion and as a promising catalyst for transesterification. This paper proposes a novel method that combines the dual functions of DBU in direct transesterification. The effects of reaction parameters on the direct transesterification of black soldier fly larvae with methanol when using DBU as both a catalyst and solvent were investigated, and a 96.2% biodiesel yield was achieved at a DBU-to-biomass ratio of 16:1 (mL/g), methanol-to-biomass ratio of 8:1 (mL/g), temperature of 110 °C, and reaction time of 60 min. Remarkably, because of its polarity reversibility, DBU was easily separated and recovered from the reaction solution through phase separation and could be repeatedly used up to 10 times without a considerable loss of catalytic activity. DBU- catalyzed direct transesterification is a green, promising, and cost-effective approach for biodiesel preparation.

 

26

Thien V. Tran, Andrei E. Siniavin, Anh N. Hoang, My T.T. Le, Chuong D. Pham, Trung V. Phung, Khoa C. Nguyen, Rustam H. Ziganshin, Victor I. Tsetlin, Ching-Feng Weng, and Yuri N. Utkin, "Phospholipase A2 from krait Bungarus fasciatus venom induces human cancer cell death in vitro", PeerJ. 2019; 7: e8055.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896944/

Abstract:
Background
Snake venoms are the complex mixtures of different compounds manifesting a wide array of biological activities. The venoms of kraits (genus Bungarus, family Elapidae) induce mainly neurological symptoms; however, these venoms show a cytotoxicity against cancer cells as well. This study was conducted to identify in Bungarus fasciatus venom an active compound(s) exerting cytotoxic effects toward MCF7 human breast cancer cells and A549 human lung cancer cells. Methods
The crude venom of B. fasciatus was separated by gel-filtration on Superdex HR 75 column and reversed phase HPLC on C18 column. The fractions obtained were screened for cytotoxic effect against MCF7, A549, and HK2 cell lines using colorimetric assay with the tetrazolium dye MTT- 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The primary structure of active protein was established by ultra high resolution LC-MS/MS. The molecular mechanism of the isolated protein action on MCF7 cells was elucidated by flow cytometry.
Results
MTT cell viability assays of cancer cells incubated with fractions isolated from B. fasciatus venom revealed a protein with molecular mass of about 13 kDa possessing significant cytotoxicity. This protein manifested the dose and time dependent cytotoxicity for MCF7 and A549 cell lines while showed no toxic effect on human normal kidney HK2 cells. In MCF7, flow cytometry analysis revealed a decrease in the proportion of Ki-67 positive cells. As Ki-67 protein is a cellular marker for proliferation, its decline indicates the reduction in the proliferation of MCF7 cells treated with the protein. Flow cytometry analysis of MCF7 cells stained with propidium iodide and Annexin V conjugated with allophycocyanin showed that a probable mechanism of cell death is apoptosis. Mass spectrometric studies showed that the cytotoxic protein was phospholipase A2. The amino acid sequence of this enzyme earlier was deduced from cloned cDNA, and in this work it was isolated from the venom as a protein for the first time. It is also the first krait phospholipase A2 manifesting the cytotoxicity for cancer cells. Keywords: Phospholipase A2, Venom, Krait, Cytotoxicity, Human cancer cells, Breast cancer, Lung cancer, Mass spectrometry
27

Thai Thanh Hoang Thi, Yunki Lee, Phuong Le Thi, Ki Dong Park, “Engineered horseradish peroxidase-catalyzed hydrogels with high tissue adhesiveness for biomedical applications”, JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 2019, 78, 34 (ISI, IF: 5.278)

https://doi.org/10.1016/j.jiec.2019.05.026

Abstract:
Adhesive hydrogels offer considerable potential in biomedical applications because of the excellent integration between materials and tissues. Horseradish peroxidase (HRP)-catalyzed hydrogelation, with advantages that include ease of handling, simplicity in material design, high biocompatibility, and processability, is becoming a promising candidate to be engineered as an adhesive hydrogel. This review summarizes recent development of HRP-mediated hydrogelation towards the bioadhesive field, and other applications requiring the tissue adhesion of hydrogels. From the viewpoint of adhesive hydrogel designation, the basic chemistry of the adhesive mechanism, combinations of interactions, and currently commercial bioglues are summarized. The review recapitulated the adhesive hydrogel applications, the clinical translation ability, the controlling hydrogelation kinetics, the related physicochemical characteristics, cytotoxicity, and animal studies. Looking to the future, to make more substantial contributions to the bioadhesive field, or to various biomedical applications, higher adhesion should be continuously improved, and greater emphasis should be placed on clinical trials.
28

Thai Thanh Hoang Thi, Yunki Lee, Phuong Le Thi & Ki Dong Park, "Oxidized Alginate Supplemented Gelatin Hydrogels for the In Situ Formation of Wound Dressing with High Antibacterial Activity", Macromolecular Research 27, (2019), 811-820 (ISI, IF: 1.758)

https://link.springer.com/article/10.1007/s13233-019-7115-3

Abstract:
The development of antibacterial wound dressing with easily removable characteristic and highly exudate absorption is becoming a major requirement in the treatment of chronic wounds. In this study, the in situ forming gelatin/oxidized alginate-tyramine hydrogels (GH/OATA) were designed to overcome the current limitations. These hybrid hydrogels were quickly and controllably formed from several seconds to a few minutes by horseradish peroxidase/H2O2. The hybrid hydrogels at the optimal composition of GH/OATA 50/50 v/v showed the unique feature including stiffness being similar to skin, high swelling ratio due to the hydrophilic property of oxidized alginate, low adhesive strength allowing for the easy dressing removal without pain. In addition, the hybrid hydrogels could release the H2O2 amount sustainably to kill bacteria. Furthermore, 3D culture of human dermal fibroblasts inside hybrid hydrogels achieved more than 80% cell proliferation which implies the cell-compatibility.
29

Nguyen Quang Khuyen, Zane Zondaka, Madis Harjo, Janno Torop, Tarmo Tamm, Rudolf Kiefer, “Comparative Analysis of Fluorinated Anions for Polypyrrole Linear Actuator Electrolytes”, Polymers 11 (2019), 849 (ISI, IF: 3.462)

https://www.mdpi.com/2073-4360/11/5/849

 

Abstract:
Either as salts or room temperature ionic liquids, fluorinated anion-based electrolytes have been a common choice for ionic electroactive polymer actuators, both linear and bending. In the present work, propylene carbonate solutions of four electrolytes of the three hugely popular anions—triflouromethanesulfonate, bis(trifluoromethane)sulfonimide, and hexafluorophosphate were compared and evaluated in polypyrrole linear actuators. The actuation direction, the characteristics—performance relations influence the behavior of the actuators. Isotonic Electro-chemo-mechanical deformation (ECMD) measurements were performed to study the response of the PPy/DBS samples. The highest strain for pristine PPy/DBS linear actuators was found in range of 21% for LiTFSI, while TBAPF6 had the least cation involvement, suggesting the potential for application in durable and controllable actuators. Interesting cation effects on the actuation of the same anions (CF3SO3−) were also observed.
30

Dieu-Hien Truong, Dinh Hieu Nguyen, Nhat Thuy Anh Ta, Anh Vo Bui, Tuong Ha Do, and Hoang Chinh Nguyen, "Evaluation of the Use of Different Solvents for phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia", Journal of Food Quality, Journal of Food Quality, vol. 2019, 2019 (IF: 1.763)

https://www.hindawi.com/journals/jfq/2019/8178294/

Abstract:
Severinia buxifolia (Rutaceae) is a promising source of bioactive compounds since it has been traditionally used for the treatment of various diseases. The present study aimed at evaluating the impact of different solvents on extraction yields, phytochemical constituents and antioxidants, and in vitro anti-inflammatory activities of S. buxifolia. The results showed that the used solvents took an important role in the yield of extraction, the content of chemical components, and the tested biological activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w). The extract obtained from methanol exhibited high capacity of antioxidant (IC50 value of 16.99 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 28.86 μg/mL; antiproteinase activity: IC50 = 414.29 μg/mL; and membrane stabilization: IC50 = 319 μg/mL). The antioxidant activity of the S. buxifolia extract was found to be 3-fold higher than ascorbic acid, and the anti-inflammatory activity of S. buxifolia extract was comparable to aspirin. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy.
31

Velmurugan, Bharath Kumar et al. “UNC13C Suppress Tumor Progression via Inhibiting EMT Pathway and Improves Survival in Oral Squamous Cell Carcinoma.” Frontiers in oncology, 9 (2019) 728 (ISI, IF: 4.848)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6694713/

Abstract:
Potential function of UNC13C in variety of cancers including, oral squamous cell carcinoma (OSCC) remains obscure. In the present study, immunohistochemical staining in tissue microarrays containing 268 OSCC samples showed that UNC13C protein levels were inversely correlated with AJCC Stage III and IV (P = 0.002) and death (P = 0.0134). Patients with lower UNC13C expression had a significantly shorter survival (P = 0.0231) than those with higher UNC13C expression. We also identified decreased overall UNC13C expression in oral cancer cell lines. In addition, our functional analysis of UNC13C shows that overexpression of UNC13C inhibited migration and invasion capacities of SCC-9 and SAS cells compared with the empty plasmid transfected cells. Further experiments suggested that transcription factors (Slug, Snail, Twist, and ZEB1) and mesenchymal marker (Vimentin) were down regulated and Tight Junction Protein (Claudin1) was up regulated after UNC13C overexpression in SCC9 and SAS cells. The novel role of UNC13C is revealed for the first time in OSCC. In summary, these results suggest that UNC13C as a novel tumor suppressor and an essential regulator of EMT signaling pathway during OSCC progression, and thus it could be used as a target for preventing oral cancer metastasis.
32

N. T. Nguyen, T. H. M. Phan, T. N. Tran, B. K. Velmurugan and R. Kiefer. 2019. Production of novel bio-flocculants from Klebsiella variicola BF1 using cassava starch wastewater and its application. Current Sciences 117 (1), 121-129. doi: 10.18520/cs/v117/i1/121-129

Abstract:
In this study, Klebsiella variicola BF1 that uses cassa-va starch wastewater to produce flocculants was identified using 16S rDNA gene sequencing. The pure flocculants of strain BF1 could be easily extracted by ethanol precipitation with a high yield of 7.5 g/l. It was mainly composed of 83.1% carbohydrates and 10.6% proteins. The flocculating activity revealed 97.6 ± 0.6% for kaolin suspension at 12.8 mg/l extracted flocculants from strain BF1 and 2.5 g/l CaCl2. Inter-estingly, the flocculating activity was 78% without the addition of metal ions. Furthermore, flocculants of strain BF1 can be effectively applied in the treatment of cassava starch wastewater and municipal wastewater.
33

Harjo M, Torop J, Järvekülg M, Tamm T, Kiefer R. "Electrochemomechanical Behavior of Polypyrrole-Coated Nanofiber Scaffolds in Cell Culture Medium", Polymers (Basel) 11(6), (2019), 1043. (ISI, IF: 3.426)

https://www.mdpi.com/2073-4360/11/6/1043

Abstract:
Glucose-gelatin nanofiber scaffolds were made conductive and electroactive by chemical (conductive fiber scaffolds, CFS) and additionally electrochemical polypyrrole deposition (doped with triflouromethanesulfonate CF3SO3−, CFS-PPyTF). Both materials were investigated in their linear actuation properties in cell culture medium (CCM), as they could be potential electro-mechanically activated cell growth substrates. Independent of the deposition conditions, both materials showed relatively stable cation-driven actuation in CCM, based on the flux of mainly Na+ ions from CCM. The surprising result was attributed to re-doping by sulfate anions in CCM, as also indicated by energy-dispersive X-ray (EDX) spectroscopy results. Overall, the electrochemically coated material outperformed the one with just chemical coating in conductivity, charge density and actuation response.
34

Harjo M, Tamm T, Anbarjafari G, Kiefer R. "Hardware and Software Development for Isotonic Strain and Isometric Stress Measurements of Linear Ionic Actuators", Polymers (Basel) 11(6), (2019), 1054. (ISI, IF: 3.426)

https://www.mdpi.com/2073-4360/11/6/1054

Abstract:
An inseparable part of ionic actuator characterization is a set of adequate measurement devices. Due to significant limitations of available commercial systems, in-house setups are often employed. The main objective of this work was to develop a software solution for running isotonic and isometric experiments on a hardware setup consisting of a potentiostat, a linear displacement actuator, a force sensor, and a voltmeter for measuring the force signal. A set of functions, hardware drivers, and measurement automation algorithms were developed in the National Instruments LabVIEW 2015 system. The result is a software called isotonic (displacement) and isometric (force) electro-chemo-measurement software (IIECMS), that enables the user to control isotonic and isometric experiments over a single compact graphical user interface. The linear ionic actuators chosen as sample systems included different materials with different force and displacement characteristics, namely free-standing polypyrrole films doped with dodecylbenzene sulfonate (PPy/DBS) and multiwall carbon nanotube/carbide-derived carbon (MWCNT-CDC) fibers. The developed software was thoroughly tested with numerous test samples of linear ionic actuators, meaning over 200 h of experimenting time where over 90% of the time the software handled the experiment process autonomously. The uncertainty of isotonic measurements was estimated to be 0.6 µm (0.06%). With the integrated correction algorithms, samples with as low as 0 dB signal-to-noise ratio (SNR) can be adequately described.
35

Cuong V. Nguyen, Hiromichi Nakahara, Osamu Shibata, Chi M.Phan, "Adsorption of sodium iodine at air/water interface", Journal of Molecular Liquids (2020) (ISI, IF: 4.561) https://www.sciencedirect.com/science/article/abs/pii/S0167732219348329

Abstract:
The change in surface potential was measured for NaI solutions. The modelled surface charge was then calculated and compared with molecular simulations. It was found that I− was enhanced at the air/water interface more than Na+. The result, which was confirmed by simulations, was opposite to the previous observation with NaCl. The trend is also consistent with anionic effects: larger and more polar anions adsorbed stronger at the air/water interface. The theoretical model was applied successfully to describe the changes for both systems, which are positive for NaCl and negative for NaI, respectively. The combined results of the two systems also revealed that the self-ionization of pure water induced a positive surface charge at 16.9 mV.
36

Muhannad K. Zabar, Cuong V. Nguyen*, Chi M. Phan, "Quantifying the influence of salinity on spontaneous emulsification of hydrocarbons", Colloids and Surfaces A: Physicochemical and Engineering Aspects, (2020) (ISI, IF: 3.131)

Abstract:
This study has investigated the process of oil-in-water spontaneous emulsification using the hydrophobic force of a non-ionic surfactant (Triton X-114) and inorganic salt additive (NaCl). The cloud point of surfactant solutions with different salt concentrations was examined and show a gradual decrease from 27 °C to 18.5 °C when increasing the salinity from 0 to 5 M. The adsorption of Triton X-114 into the oil-water interface has spontaneously enlarged the surface excess concentration of oil droplet in the system, leading to the decrease in surface tension and the spontaneous formation of oil droplets in water. Increasing the concentration of salt additive caused an increment in ions’ penetration into the hydrophilic layer of surfactants, resulting in the formation of smaller droplets. Increasing the chain-length of the oil from C7 (n-heptane) to C16 (n-hexadecane) produced a decrease of 58.6 % in droplet diameter. According, a newly-proposed model was developed and fitted against experimental data to obtain the best-fitted parameters of maximum droplet size (D0) and ion adsorbent constant (Kion). The data and modelling results verify the influence of the interfacial layer on the emulsions’ size and stability.
37

Thai Thanh Hoang Thi, Emily H. Pilkington, Dai Hai Nguyen, Jung Seok Lee, Ki Dong Park and Nghia P. Truong, "The Importance of Poly(ethylene glycol) Alternatives for Overcoming PEG Immunogenicity in Drug Delivery and Bioconjugation", Polymers (Basel). 12(2), (2020), 298 (ISI, IF: 3.426) https://www.mdpi.com/2073-4360/12/2/298

Abstract:
Poly(ethylene glycol) (PEG) is widely used as a gold standard in bioconjugation and nanomedicine to prolong blood circulation time and improve drug efficacy. The conjugation of PEG to proteins, peptides, oligonucleotides (DNA, small interfering RNA (siRNA), microRNA (miRNA)) and nanoparticles is a well-established technique known as PEGylation, with PEGylated products have been using in clinics for the last few decades. However, it is increasingly recognized that treating patients with PEGylated drugs can lead to the formation of antibodies that specifically recognize and bind to PEG (i.e., anti-PEG antibodies). Anti-PEG antibodies are also found in patients who have never been treated with PEGylated drugs but have consumed products containing PEG. Consequently, treating patients who have acquired anti-PEG antibodies with PEGylated drugs results in accelerated blood clearance, low drug efficacy, hypersensitivity, and, in some cases, life-threatening side effects. In this succinct review, we collate recent literature to draw the attention of polymer chemists to the issue of PEG immunogenicity in drug delivery and bioconjugation, thereby highlighting the importance of developing alternative polymers to replace PEG. Several promising yet imperfect alternatives to PEG are also discussed. To achieve asatisfactory alternative, further joint efforts of polymer chemists and scientists in related fields are urgently needed to design, synthesize and evaluate new alternatives to PEG.
38

Phuong Le Thi, Yunki Lee, Dieu Linh Tran, Ki Dong Park, Horseradish peroxidase-catalyzed hydrogelation of fish gelatin with tunable mechanical properties and biocompatibility. Journal of Biomaterials Applications, 34(9), 1216–1226. (ISI, IF: 2.764)

https://doi.org/10.1177/0885328219899787

Abstract:
Horseradish peroxidase-catalyzed injectable gelatin hydrogels have attracted much attention in various biomedical fields because of their processability, biodegradability, and excellent biocompatibility in promoting cell adhesion and proliferation. However, gelatin derivatives are mainly obtained from mammalian sources (porcine, bovine) with thermal gelation at room temperature, leading to the potential problems in biofabrication applications. Here, we introduce a novel fish gelatin derivative that can be easily dissolved and cross-linked at room temperature by horseradish peroxidase. This system provides thermally stable fish gelatin hydrogels with tunable mechanical and biological properties, comparable to porcine gelatin hydrogels. The properties (gelation time, stiffness, degradation rate) of hydrogels prepared from fish gelatin-hydroxyphenyl propionic acid (FGH) are controllable for suitable applications. Moreover, FGH hydrogels allow human dermal fibroblast cells to adhere, proliferate, and produce the extracellular components. These results suggest horseradish peroxidase-cross-linked FGH as potential hydrogel matrices that can be used as an alternative for mammalian gelatin hydrogels in various biomedical applications.
39

Kesküla, A.; Heinmaa, I.; Tamm, T.; Aydemir, N.; Travas-Sejdic, J.; Peikolainen, A.-L.; Kiefer, R. "Improving the Electrochemical Performance and Stability of Polypyrrole by Polymerizing Ionic Liquids" Polymers 12 (2020) 136. (ISI, IF: 3.426)

https://www.mdpi.com/2073-4360/12/1/136

Abstract:
Polypyrrole (PPy) based electroactive materials are important building blocks for the development of flexible electronics, bio-sensors and actuator devices. As the properties and behavior of PPy depends strongly on the operating environment—electrolyte, solvent, etc., it is desirable to plant immobile ionic species into PPy films to ensure stable response. A premade ionic polymer is not optimal in many cases, as it enforces its own structure on the conducting polymer, therefore, polymerization during fabrication is preferred. Pyrrole (Py) was electropolymerized at low temperature together with a polymerizable ionic liquid (PIL) monomer in a one-step polymerization, to form a stable film on the working electrode. The structure and morphology of the PPyPIL films were investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), Fourier-transform infrared (FTIR) spectroscopy and solid-state NMR (ssNMR) spectroscopy. The spectroscopy results confirmed the successful polymerization of Py to PPy and PIL monomer to PIL. The presence of (TFSI–) anions that balance the charge in PPyPIL was confirmed by EDX analysis. The electrical properties of PPyPIL in lithium bis(trifluoromethanesulfonyl)-imide (LiTFSI) aqueous and propylene carbonate solutions were examined with cyclic voltammetry (CV), chronoamperometry, and chronopotentiometry. The blend of PPyPIL had mixed electronic/ionic conductive properties that were strongly influenced by the solvent. In aqueous electrolyte, the electrical conductivity was 30 times lower and the diffusion coefficient 1.5 times higher than in the organic electrolyte. Importantly, the capacity, current density, and charge density were found to stay consistent, independent of the choice of solvent.
40

Ngoc Thuy Trang Le, Dai Hai Nguyen, Ngoc Hoi Nguyen, Yern Chee Ching, Dong Yen Pham Nguyen, Cuong Quoc Ngo, Hang Nguyen Thi Nhat andThai Thanh Hoang Thi, Silver Nanoparticles Ecofriendly Synthesized by Achyranthes aspera and Scoparia dulcis Leaf Broth as an Effective Fungicide. Applied Sciences 2020, 10, 2505. (ISI, IF 2.474)

https://www.mdpi.com/2076-3417/10/7/2505

Abstract:
This study describes an inexpensive, simple and green method to form silver nanoparticles from different leaf extracts of Achyranthes aspera and Scoparia dulcis plants. The silver nitrate is reduced by Achyranthes aspera and Scoparia dulcis leaf extracts respectively to generate two silver nanoparticle types symbolized as AA.AgNPs and SD.AgNPs. The optical absorption, size and morphology of silver nanoparticles are significantly impacted by extract types. The ultraviolet visible spectrum of AA.AgNPs shows a 433-nm peak being more broadened than that of SD.AgNPs. The Fourier infrared transform spectra of two of these silver nanoparticles revealed that their surface is modified by organic constituents from extracts, and thus they are stabilized in solution without any additional reaction. Images from transmission electron microscopy and scanning electron microscope indicate that AA.AgNPs are in clusters with the size of 8–52 nm almost possessing oval shape, while SD.AgNPs are smaller size of 5-45 nm separated well in diversified shapes (spherical, triangle, quadrilateral and hexagonal). Moreover, both AA.AgNPs and SD.AgNPs exhibit the highly antifungal effect against Aspergillus niger, Aspergillus flavus and the most strong impact on Fusarium oxysporum. For these obtained results, two new silver nanoparticles are promising fungicides for various applications of medical and agricultural fields.
41

Dinh-Chuong Pham, Hoang-Chinh Nguyen, Thanh-Hang Le Nguyen, Hoang-Linh Ho, Thien-Kim Trinh, Jirawat Riyaphan, and Ching-Feng Weng, "Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities", BioMed Research International, Volume 2020 |Article ID 3497107

https://www.hindawi.com/journals/bmri/2020/3497107/

Abstract:
Celastrus hindsii is a potential source of flavonoids with biological activities. This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%. The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities. The results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively. Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line. This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves.
42

Quoc Hai Nguyen, Seongjoon So, Quoc Hanh Nguyen, Il Tae Kim, Jaehyun Hur, "Mechanochemical synthesis of InP nanoparticles embedded in hybrid conductive matrix for high-performance lithium-ion batteries", Chemical Engineering Journal, 399 (2020) 125826 (ISI IF: 10.652.

https://www.sciencedirect.com/science/article/abs/pii/S1385894720319549

Abstract:
InP nanoparticles distributed in a TiO2-C hybrid matrix (InP@TiO2-C) are proposed as a promising anode material for Li-ion batteries. A primary mechanochemical process on the precursor materials (In2O3, Ti, and P) leads to the formation of InP and TiO2 nanocrystal particles. The introduction of carbon during secondary ball milling results in the formation of InP nanoparticles that are embedded in the TiO2-C hybrid conductive matrix (InP@TiO2-C); this is confirmed by X-ray diffraction (XRD), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray characterizations. As an anode material, InP@TiO2-C exhibits impressive electrochemical performance in both half- and full-cell batteries. Various ex situ analyses including XRD, high-resolution TEM (HRTEM), and XPS were synergistically used to demonstrate the mechanism of lithium-ion storage for the InP@TiO2-C electrode during the electrochemical reaction, and to elucidate the roles of active InP particles and the TiO2-C buffering hybrid matrix. Consequently, as an anode for half-cell batteries, the InP@TiO2-C delivers a high reversible capacity (~850 mAh g−1 after 120 cycles at 0.1 mA g−1), excellent life span at 0.5 A g−1 (~750 mAh g−1 after 800 cycles), and high rate capability (85% capacity retention at 10 A g−1 compared with that at 0.1 A g−1). Moreover, as an anode for practical full-cell batteries with LiFePO4@graphite cathodes, it delivers a promising initial energy density of 214 Wh kg−1 (based on the total mass of the anode and cathode) and a good stability (retention of 61%) over 150 cycles.
43

Nguyen Quang Khuyen, Rudolf Kiefer, Fred Elhi, Gholamreza Anbarjafari, Jose G Martinez, Tarmo Tamm, “A Biomimetic Approach to Increasing Soft Actuator Performance by Friction Reduction”, Polymers 12 (2020), 1120 (ISI, IF: 3.462). https://www.mdpi.com/2073-4360/12/5/1120/htm

Abstract:
While increasing power output is the most straight-forward solution for faster and stronger motion in technology, sports, or elsewhere, efficiency is what separates the best from the rest. In nature, where the possibilities of power increase are limited, efficiency of motion is particularly important; the same principle can be applied to the emerging biomimetic and bio-interacting technologies. In this work, by applying hints from nature, we consider possible approaches of increasing the efficiency of motion through liquid medium of bilayer ionic electroactive polymer actuations, focusing on the reduction of friction by means of surface tension and hydrophobicity. Conducting polyethylene terephthalate (PET) bilayers were chosen as the model actuator system. The actuation medium consisted of aqueous solutions containing tetramethylammonium chloride and sodium dodecylbenzenesulfonate in different ratios. The roles of ion concentrations and the surface tension are discussed. Hydrophobicity of the PET support layer was further tuned by adding a spin-coated silicone layer to it. As expected, both approaches increased the displacement—the best results having been obtained by combining both, nearly doubling the bending displacement. The simple approaches for greatly increasing actuation motion efficiency can be used in any actuator system operating in a liquid medium.
44

S.Arokiyaraj, R.Varghese, B.Ali Ahmed, V.Duraipandiyan, N.A.Al-Dhabi, "Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment", 26 (2019), 378-381 (ISI, IF: 2.802)

https://www.sciencedirect.com/science/article/pii/S1319562X18302407

Abstract:
Keratinase degrading Bacillus cereus was isolated from the halophilic environment in Tamilnadu, India and keratinase production was optimized using wheat bran substrate. Of the screened bacterial isolates, four were found to have the ability to produce keratinolytic enzyme. The process parameters were optimized using one-variable-at-a-time approach and response surface methodology. Supplementation of 1% lactose supported more keratinase production (120 U/g). Among the selected nitrogen sources, addition of casein significantly enhanced maximum keratinase production (132.5 U/g). Among the ions, manganese chloride significantly enhanced keratinsase production (102.6 U/g), however addition of zinc sulphate and copper sulphate decreased keratinase production. The maximum keratinase production was obtained in the wheat bran medium containing 1% lactose, 0.5% manganese with 80% moisture (292 U/g). Statistics based contour plots were generated to explore the variations in the response surface and to find the relationship between the keratinase yield and the bioprocess conditions.
45

Thai Thanh Hoang Thi, Diem-Huong Tran Nguyen, D.T.D Nguyen, Dai Hai Nguyen, Minh-Dung Truong, "Decellularized Porcine Epiphyseal Plate-Derived Extracellular Matrix Powder: Synthesis and Characterization", Cells Tissues Organs, (2020), DOI: 10.1159/000507552 (ISI, IF: 2.064)

https://www.karger.com/Article/Abstract/507552

Abstract:
The aim of this study was to develop a porcine epiphyseal plate-derived extracellular matrix powder (PEPEP) for epiphyseal plate regeneration. PEPEP was characterized by chemical assay to determine the contents of DNA and epiphyseal plate complex chemical components (glycosaminoglycan and hydroxyproline). The effects of PEPEP on the viability, proliferation, and differentiation of human bone marrow mesenchymal stem cells (hBMSCs) were also evaluated. hBMSCs cultured in PEPEP exhibited a good distribution with excellent viability after 72 h, demonstrating the ability of PEPEP to support hBMSC proliferation. At week 4 and 6 in vitro, the PEPEP + hBMSCs structure showed chondrogenic ability and an increase in expression of collagen type I, type II, and type X. PEPEP showed a promising ability to enhance cartilage formation and promote chondrocyte differentiation, maturation, and hypertrophy. The results provide insights into the feasibility of PEPEP as a potential material for tissue engineering applications.
46

Hoang Chinh Nguyen, Fu-Ming Wang, Kim Khue Dinh, Thanh Truc Pham, Horng-Yi Juan, Nguyen Phuong Nguyen, Hwai Chyuan Ong, Chia-Hung Su, "Microwave-Assisted Noncatalytic Esterification of Fatty Acid for Biodiesel Production: A Kinetic Study", Energies, 13, 9, (2020), 2167 (ISI, IF: 2.702)

https://www.mdpi.com/1996-1073/13/9/2167

Abstract:
This study developed a microwave-mediated noncatalytic esterification of oleic acid for producing ethyl biodiesel. The microwave irradiation process outperformed conventional heating methods for the reaction. A highest reaction conversion, 97.62%, was achieved by performing esterification with microwave irradiation at a microwave power of 150 W, 2:1 ethanol:oleic acid molar ratio, reaction time of 6 h, and temperature of 473 K. A second-order reaction model (R2 of up to 0.997) was established to describe esterification. The reaction rate constants were promoted with increasing microwave power and temperature. A strong linear relation of microwave power to pre-exponential factors was also established, and microwave power greatly influenced the reaction due to nonthermal effects. This study suggested that microwave-assisted noncatalytic esterification is an efficient approach for biodiesel synthesis
47

Dinh Tien Dung Nguyen, Long Giang Bach, Thi Hiep Nguyen, Minh Hieu Ho, Minh Nhat Ho, Dai Hai Nguyen, Cuu Khoa Nguyen & Thai Thanh Hoang Thi, Preparation and characterization of oxaliplatin drug delivery vehicle based on PEGylated half-generation PAMAM dendrimer", Journal of Polymer Research, 26, 2019), 116 (ISI, IF: 2.426)

ttps://doi.org/10.1007/s10965-019-1779-4

Abstract:
Dendrimers were well-known as a polymeric nanoparticle drug carrier system. Among them, polyamidoamine (PAMAM) dendrimers were firstly and systematically studied. Herein, to explore the additional modification of PAMAM for drug deliver application, this study assessed the PEGylated half-generation G3.5 to load oxaliplatin (OXA). The proton nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FTIR) spectroscopy were used to confirm the successful synthesis of G3.5, and PEGylated G3.5. PEG modification on G3.5 neutralized the negative charge of G3.5 that was confirmed by zeta potential measurement, and increased the dimension of G3.5 from 10 to 100 nm that was carried out by TEM technique. G3.5-PEG showed the high drug loading efficiency of 75.69%. The release kinetic of OXA from G3.5-PEG@OXA indicated that no burst released phenomenon occurred (11.95% within first hour) and sustainable release was achieved. In cytotoxicity test with normal cells of L929 fibroblasts, the carrier system of G3.5-PEG did not induced any cytotoxicity. To test the killing effect of G3.5-PEG@OXA on cancerous cells of human cervical cancer cells (HeLa), lung adenocarcinoma (A549), and breast cancer (MCF-7), resazurin test and live/dead staining assay was used to observe the alive cells. The increase of OXA amount in G3.5-PEG@OXA lead to decrease the cell viability from 79.90–56.97% (HeLa), 84.80–64.00% (A549), and 92.00–65.00% (MCF-7) after 48 h treatment.
48

Thai Thanh Hoang Thi, Diem-Huong Nguyen Tran, Long Giang Bach, Hieu Vu-Quang, Duy Chinh Nguyen, Ki Dong Park, Dai Hai Nguyen, Functional Magnetic Core-Shell System-Based Iron Oxide Nanoparticle Coated with Biocompatible Copolymer for Anticancer Drug Delivery. Pharmaceutics. 2019;11(3):120. (ISI, IF: 4.421) https://www.mdpi.com/1999-4923/11/3/120

Abstract:
Polymer coating has drawn increasing attention as a leading strategy to overcome the drawbacks of superparamagnetic iron oxide nanoparticles (SPIONs) in targeted delivery of anticancer drugs. In this study, SPIONs were modified with heparin-Poloxamer (HP) shell to form a SPION@HP core-shell system for anticancer drug delivery. The obtained formulation was characterized by techniques including transmission electron microscopy (TEM), Fourier transform infrared spectra (FT-IR), vibration sample magnetometer (VSM), proton nuclear magnetic resonance (¹H-NMR), and powder X-ray diffraction (XRD). Results showed the successful attachment of HP shell on the surface of SPION core and the inability to cause considerable effects to the crystal structure and unique magnetic nature of SPION. The core-shell system maintains the morphological features of SPIONs and the desired size range. Notably, Doxorubicin (DOX), an anticancer drug, was effectively entrapped into the polymeric shell of SPION@HP, showing a loading efficiency of 66.9 ± 2.7% and controlled release up to 120 h without any initial burst effect. Additionally, MTT assay revealed that DOX-loaded SPION@HP exerted great anticancer effect against HeLa cells and could be safely used. These results pave the way for the application of SPION@HP as an effective targeted delivery system for cancer treatment.
49

Dai Hai Nguyen, Jung Seok Lee, Ki Dong Park, Yern Chee Ching, Xuan Nguyen Thi, V.H. Giang Phan, Thai Thanh Hoang Thi, "Green Silver Nanoparticles Formed by Phyllanthus urinaria, Pouzolzia zeylanica, and Scoparia dulcis Leaf Extracts and the Antifungal Activity", 10(3), (2020), 542 (ISI, IF: 4.324)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153602/

Abstract:
Phytoconstituents presenting in herbal plant broths are the biocompatible, regenerative, and cost-effective sources that can be utilized for green synthesis of silver nanoparticles. Different plant extracts can form nanoparticles with specific sizes, shapes, and properties. In the study, we prepared silver nanoparticles (P.uri.AgNPs, P.zey.AgNPs, and S.dul.AgNPs) based on three kinds of leaf extracts (Phyllanthus urinaria, Pouzolzia zeylanica, and Scoparia dulcis, respectively) and demonstrated the antifungal capacity. The silver nanoparticles were simply formed by adding silver nitrate to leaf extracts without using any reducing agents or stabilizers. Formation and physicochemical properties of these silver nanoparticles were characterized by UV-vis, Fourier transforms infrared spectroscopy, scanning electron microscope, transmission electron microscope, and energy dispersive X-ray spectroscopy. P.uri.AgNPs were 28.3 nm and spherical. P.zey.AgNPs were 26.7 nm with hexagon or triangle morphologies. Spherical S.dul.AgNPs were formed and they were relatively smaller than others. P.uri.AgNPs, P.zey.AgNPs and S.dul.AgNPs exhibited the antifungal ability effective against Aspergillus niger, Aspergillus flavus, and Fusarium oxysporum, demonstrating their potentials as fungicides in the biomedical and agricultural applications.
50

Ngoc Thuy Trang Le, Thi Nhu Quynh Nguyen, Van Du Cao, Duc Thuan Hoang, Van Cuong Ngo, Thai Thanh Hoang Thi, "Recent Progress and Advances of Multi-Stimuli-Responsive Dendrimers in Drug Delivery for Cancer Treatment." Pharmaceutics 2019, 11, 591. (ISI, IF: 4.421) https://www.mdpi.com/1999-4923/11/11/591

Abstract:
Despite the fact that nanocarriers as drug delivery systems overcome the limitation of chemotherapy, the leakage of encapsulated drugs during the delivery process to the target site can still cause toxic effects to healthy cells in other tissues and organs in the body. Controlling drug release at the target site, responding to stimuli that originated from internal changes within the body, as well as stimuli manipulated by external sources has recently received significant attention. Owning to the spherical shape and porous structure, dendrimer is utilized as a material for drug delivery. Moreover, the surface region of dendrimer has various moieties facilitating the surface functionalization to develop the desired material. Therefore, multi-stimuli-responsive dendrimers or ‘smart’ dendrimers that respond to more than two stimuli will be an inspired attempt to achieve the site-specific release and reduce as much as possible the side effects of the drug. The aim of this review was to delve much deeper into the recent progress of multi-stimuli-responsive dendrimers in the delivery of anticancer drugs in addition to the major potential challenges.
51

Cuong V. Nguyen, Hiromichi Nakahara, and Chi M. Phan, "Surface Potential of the Air/Water Interface", Journal of Oleo Science (2020) (ISI, IF: 1.208)

https://www.jstage.jst.go.jp/article/jos/69/6/69_ess20024/_article

Abstract:
The surface charge/surface potential of the air/water interface plays a key role in many natural and industrial processes. Since the first decade of the 20th century, there are many theoretical proposals to describe the surface charge in the presence of different moieties. However, a complete and consistent description of the interfacial layer remains elusive. More recently, the theoretical frameworks and experimental data get complementary support from the simulation at a molecular level. This paper reviews the recent developments from the theoretical, experimental and simulation aspects. The combined results indicated that the interaction between hydration shells of adsorbed ions and the H-bonds network of surface water plays a critical role in the ionic adsorption. The factor should be incorporated into the conventional theories to correctly predict the ion distribution near the air/water surface.
52

Ngoc Thuy Trang Le, Binh T.D. Trinh, Dai Hai Nguyen, Lam Dai Tran, Cuong Hung Luu, Thai Thanh Hoang Thi, "The Physicochemical and Antifungal Properties of Eco-friendly Silver Nanoparticles Synthesized by Psidium guajava Leaf Extract in the Comparison With Tamarindus indica" Journal of Cluster Science (2020) https://doi.org/10.1007/s10876-020-01823-6

Abstract:
The green synthesized silver nanoparticles get much attention because of less toxicity, friendly environmental method, and their potential properties in various applications. Herein, the silver nanoparticles named as P.Gua-AgNPs or T.Ind-AgNPs are respectively fabricated by the oxidation–reduction reactions between silver ion and Psidium guajava or Tamarindus indica leaf extract. The Ultraviolet–visible spectrum of P.Gua-AgNPs appears the 429-nm peak while the peak of T.Ind-AgNPs is at 491 nm. The transmission electron microscopy and scanning electron microscope images show the P.Gua-AgNPs are spherical, triangular and plate-like polyshaped morphology in 5–53 nm, the T.Ind-AgNPs have spherical shape in 12–91 nm. The Fourier transform infrared spectra reveal that the particle surface contains the organic functional groups that is consisted with the elemental analysis from the energy dispersive X-ray analysis indicating the presence of C, O and Ag. So the Psidium guajava leaf extract containing abundant polyphenols, polyols, aldehyde content, and sulfur compounds creates the P.Gua-AgNPs with smaller size, narrower distribution, more diversified morphology than T.Ind-AgNPs fabricated from Tamarindus indica leaf extract being rich of organic acid and vitamin C. In addition, T.Ind-AgNPs and P.Gua-AgNPs show the highly antifungal ability against Fusarium oxysporum, Aspergillus niger, and Aspergillus flavus.
53

Velmurugan BK, Wang HK, Chung CM, et al. "CIP2A overexpression in Taiwanese oral cancer patients" Cancer Management and Research,11 (2019) 2589-2594. (ISI, IF: 2.886) https://www.dovepress.com/cip2a-overexpression-in-taiwanese-oral-cancer-patients-peer-reviewed-article-CMAR

Abstract:
Introduction: Oral cancer is a prevalent form of cancer worldwide, particularly in Taiwan, and mechanisms involved in oral squamous cell carcinoma (OSCC) progression remain relatively unknown. Cancerous inhibitor of protein phosphatase 2A (CIP2A), an oncoprotein, is aberrantly expressed in many human malignant tumors including oral cancer. However, the expression and role played by CIP2A in oral cancer pathogenesis remain obscure. Methods: In this study, immunohistochemistry was used to analyze CIP2A expression between OSCC tissues and their adjacent noncancerous tissues. Furthermore, associations between CIP2A expression and histopathological parameters were investigated. Results: In this study, we showed that CIP2A was overexpressed in most of the OSCC tissues. High CIP2A expression was significantly associated with moderate/poor tumor differentiation (P=0.02). No significant association was found between CIP2A expression and other clinical parameters. Kaplan–Meier analysis revealed that high CIP2A expression showed poorer survival rates than those with low CIP2A expression (P=0.047). Multivariate Cox regression analysis indicated that CIP2A expression, N stage, American Joint Committee on Cancer stage and clinical therapy were independent prognostic factors for survival. Conclusion: Thus, our study suggests that CIP2A is an independent prognostic marker for OSCC and a novel target for OSCC treatment.
54

Lin SH, Wang HK, Yeh KT, Tai HC, Wang HY, Huang LR, Chiu CW, Chung CM, Velmurugan BK. "c-MYC expression in T (III/IV) stage oral squamous cell carcinoma (OSCC) patients" Cancer Manag Res. 11 (2019) 5163-5169 (ISI, IF: 2.886). https://doi.org/10.2147/CMAR.S201943

Abstract:
Purpose: c-MYC has been noted in many tumor types, but its functional significance and clinical utility in oral squamous cell carcinoma (OSCC) are not well known. Here we studied the expression of c-MYC in correlation to clinical outcome in patients with oral squamous cell carcinoma. Methods: The current study, using immunohistochemical staining, first examined c-MYC expression in OSCC patients and further correlated its expression with clinicopathological parameters. Results: c-MYC was expressed in the majority of OSCC patients (n=133). The c-MYC expression is associated with histological grade (P=0.0205) of patients with oral squamous cell carcinoma. Multivariate Cox regression analysis revealed that TN stage (P<0.001),American Joint Committee on Cancer(AJCC) stage (P<0.0001), and tumor differentiation (P=0.0025) were independent factors for overall survival in patients with OSCC except for c-MYC expression (P>0.05). Multiplicative-scale interaction between T stage (III/IV) and low c-MYC expression on mortality risk was identified (P=0.0233). Kaplan–Meier survival analysis demonstrated that oral cancer patients (T III/IV stage) with high c-MYC expression had better survival than those with low and medium c-MYC expression (P=0.0270). Conclusion: Our data indicate that c-MYC is a potential biomarker that can be used as a therapeutic target for treating OSCC patients with T stage (III/IV).
55

Dai Hai Nguyen, Long Giang Bach, Diem-Huong Nguyen Tran, Van Du Cao, Thi Nhu Quynh Nguyen, Thi Thu Hong Le, Thach Thao Tran, Thai Thanh Hoang Thi, "Partial Surface Modification of Low Generation Polyamidoamine Dendrimers: Gaining Insight into their Potential for Improved Carboplatin Delivery", Biomolecules, 9(6),(2019), 214 (ISI, IF: 4.082) https://www.mdpi.com/2218-273X/9/6/214

Abstract:
Carboplatin (CAR) is a second generation platinum-based compound emerging as one of the most widely used anticancer drugs to treat a variety of tumors. In an attempt to address its dose-limiting toxicity and fast renal clearance, several delivery systems (DDSs) have been developed for CAR. However, unsuitable size range and low loading capacity may limit their potential applications. In this study, PAMAM G3.0 dendrimer was prepared and partially surface modified with methoxypolyethylene glycol (mPEG) for the delivery of CAR. The CAR/PAMAM G3.0@mPEG was successfully obtained with a desirable size range and high entrapment efficiency, improving the limitations of previous CAR-loaded DDSs. Cytocompatibility of PAMAM G3.0@mPEG was also examined, indicating that the system could be safely used. Notably, an in vitro release test and cell viability assays against HeLa, A549, and MCF7 cell lines indicated that CAR/PAMAM G3.0@mPEG could provide a sustained release of CAR while fully retaining its bioactivity to suppress the proliferation of cancer cells. These obtained results provide insights into the potential of PAMAM G3.0@mPEG dendrimer as an efficient delivery system for the delivery of a drug that has strong side effects and fast renal clearance like CAR, which could be a promising approach for cancer treatment.
56

Yuh-Ming Fuh, Dinh-Chuong Pham, and Ching-Feng Weng, "Effects of Sting Plant Extracts as Penetration Enhancers on Transdermal Delivery of Hypoglycemic Compounds", Medicina 2019, 55(5), 121.

https://www.mdpi.com/1010-660X/55/5/121

Abstract:
Background and objectives: The percutaneous route is an interesting and inventive investigation field of drug delivery. However, it is challenging for drug molecules to pass through the skins surface, which is a characterized by its permeability barrier. The purpose of this study is to look at the effect of some penetration enhancers on in vivo permeation of insulin and insulin sensitizers (curcumin and rutin) through diabetes-induced mouse skin. Materials and Methods: Sting crude extracts of Dendrocnide meyeniana, Urtica thunbergiana Sieb. and Zucc, and Alocasia odora (Lodd.) Spach were used as the penetration enhancers. Mouse skin irritation was tested by smearing the enhancers for the measurements at different time points and the cell viability of the HaCaT human skin keratinocytes, which was determined by Trypan blue exclusion and MTT assays to evaluate human biosafety for these extracts after the mouse skin permeation experiments. Results: All enhancers induced a slight erythema without edema on the mouse skin that completely recovered after 6 h from the enhancer smears as compared with normal mouse skin. Furthermore, no damaged cells were found in the HaCaT keratinocytes under sting crude extract treatments. The blood sugar level in the diabetic mice treated with the insulin or insulin sensitizers, decreased significantly (p < 0.05) in the presence of enhancers. The area under the curve (AUC) values of transdermal drug delivery (TDD) ranged from 42,000 ± 5000 mg/dL x min without enhancers, to 30,000 ± 2000 mg/dL x min in the presence of enhancers. Conclusions: This study exhibited that natural plant extracts could be preferred over the chemically synthesized molecules and are safe and potent penetration enhancers for stimulating the transdermal absorption of drugs. Keywords: transdermal; permeation enhancers; skin permeation; oral glucose tolerance test; diabetes mellitus
57

 

B. Mahalakshmi, Rathinasamy Baskaran, M. Shanmugavadivu, Ngoc Tuan Nguyen & Bharath Kumar Velmurugan "Insulinoma-associated protein 1 (INSM1): a potential biomarker and therapeutic target for neuroendocrine tumors" Cellular Oncology 43 (2020) 367–376 . (ISI, IF: 5.304) https://doi.org/10.1007/s13402-020-00505-9

Background
Insulinoma-associated protein 1 (INSM1), a transcriptional regulator with a zinc-finger DNA-binding domain, has been validated as a cytoplasmic marker for neuroendocrine differentiation of tumor cells. Next to its abundant expression in the fetal pancreas, it is expressed in brain tumors, pheochromocytomas, medullary thyroid carcinomas, insulinomas and pituitary and small-cell lung carcinomas. INSM1 is not expressed in normal adult tissues and/or most non-neuroendocrine tumors. It regulates various downstream signaling pathways, including the Sonic Hedgehog, PI3K/AKT, MEK/ERK1/2, ADK, p53, Wnt, histone acetylation, LSD1, cyclin D1, Ascl1 and N-Myc pathways. Although INSM1 appears to be a subtle and specific biomarker for neuroendocrine tumors, its role in tumor development has remained unclear.
Conclusions: Here, we highlight INSMI expression, as well as its diagnostic significance and use as a therapeutic target in various neuroendocrine tumors. Targeting signaling pathways or gene expression alterations associated with INSM1 expression may be instrumental for the design of novel therapeutic strategies for neuroendocrine tumors.
58

Velmurugan, B.K.; Lin, J.-T.; Mahalakshmi, B.; Chuang, Y.-C.; Lin, C.-C.; Lo, Y.-S.; Hsieh, M.-J.; Chen, M.-K. "Luteolin-7-O-Glucoside Inhibits Oral Cancer Cell Migration and Invasion by Regulating Matrix Metalloproteinase-2 Expression and Extracellular Signal-Regulated Kinase Pathway" Biomolecules. 10(4) (2020) 502. (ISI, IF: 4.082)

https://www.mdpi.com/2218-273X/10/4/502

Abstract:
Oral squamous cell carcinoma is the sixth most common type of cancer globally, which is associated with high rates of cancer-related deaths. Metastasis to distant organs is the main reason behind worst prognostic outcome of oral cancer. In the present study, we aimed at evaluating the effects of a natural plant flavonoid, luteolin-7-O-glucoside, on oral cancer cell migration and invasion. The study findings showed that in addition to preventing cell proliferation, luteolin-7-O-glucoside caused a significant reduction in oral cancer cell migration and invasion. Mechanistically, luteolin-7-O-glucoside caused a reduction in cancer metastasis by reducing p38 phosphorylation and downregulating matrix metalloproteinase (MMP)-2 expression. Using a p38 inhibitor, SB203580, we proved that luteolin-7-O-glucoside exerts anti-migratory effects by suppressing p38-mediated increased expression of MMP-2. This is the first study to demonstrate the luteolin-7-O-glucoside inhibits cell migration and invasion by regulating MMP-2 expression and extracellular signal-regulated kinase pathway in human oral cancer cell. The study identifies luteolin-7-O-glucoside as a potential anti-cancer candidate that can be utilized clinically for improving oral cancer prognosis.
59

Thai Thanh Hoang Thi, Le Hoang Sinh, Dai Phu Huynh, Dai Hai Nguyen, Cong Huynh, "Self-Assemblable Polymer Smart-Blocks for Temperature-Induced Injectable Hydrogel in Biomedical Applications" 8:19 (2020) (ISI, IF: 3.693)

https://www.frontiersin.org/articles/10.3389/fchem.2020.00019/full

Abstract:
Self-assembled temperature-induced injectable hydrogels fabricated via self-assembly of polymer smart-blocks have been widely investigated as drug delivery systems and platforms for tissue regeneration. Polymer smart-blocks that can be self-assembly play an important role in fabrication of hydrogels because they can self-assemble to induce the gelation of their copolymer in aqueous solution. The self-assembly occurs in response to an external stimulus change, such as temperature, pH, glucose, ionic strength, light, magnetic field, electric field, or their combination, which results in property transformations like hydrophobicity, ionization, and conformational change. The self-assembly smart-block based copolymers exist as a solution in aqueous media at certain conditions that are suitable for mixing with bioactive molecules and/or cells. However, this solution turns into a hydrogel due to the self-assembly of the smart-blocks under exposure to an external stimulus change in vitro or injection into the living body for a controllable release of loaded bioactive molecules or serving as a biomaterial scaffold for tissue regeneration. This work reports current scenery in the development of these self-assembly smart-blocks for fabrication of temperature-induced injectable physically cross-linked hydrogels and their potential application as drug delivery systems and platforms for tissue engineering.
60

Thien Khanh Tran, Hoang Jyh Leu, Trong Quyet Vu, Minh Tam Nguyen, Tuyet Anh Pham, Rudolf Kiefer, " Hydrogen production from the tannery wastewater treatment by using agriculture supports membrane/adsorbents electrochemical system, International Journal of Hydrogen Energy ", Volume 45, Issue 6, (2020), 3699-3711, (ISI, IF 4.939)

https://doi.org/10.1016/j.ijhydene.2019.05.040.

Abstract:
The tannery is one of the oldest and most popular industries in the world. It is also characterized as pollutants generated industries which discharge toxic chemical output effluents to the environment. The process of tannery included a wide variety of chemical and inorganic constituents. This work focused on the removal of Chromium heavy metals as well as producing clean energy from the treatment of tannery wastewater. Accordingly, the analysis result on the input effluents appeared to have a brown color with a very high COD value, a high concentration of Chromium heavy metals as well as other organic compounds. After collected from the source, the effluents were settling and applied a simple subsequent filtration with lab scale cloth filter, the filtered effluents then were treated in an electrochemical system. Throughout many experiments, we introduce an electrochemical system with 5 × 5 cm electrodes (Platinum coated panel anode and carbon fiber cloth cathode), the low input voltage (10 V), easy setup and the support of separator membrane/adsorbents placed in the middle of the system to enhance the removal rates of heavy metals. It was found that the performance of the electrochemical system is under the influence of various factors such as temperature, pH value, adsorbents dose, and apply voltage. During 48 h of treatment, almost 80% of Chromium metals were treated by means of adsorption and electrical reduction process. The generation rate of hydrogen gases during the electrolysis process was also notable (45–65 cc/min). Furthermore, the adsorbent materials were still intact and seem to be ready for a longer run. The study also observed the adsorbent membrane of bagasse and straw showed their best removal efficiencies over other candidates. In that manner, we successfully provide an effective method for heavy metals removal and also capable of generating clean energy. The analysis results clarify that most of the parameters of the physical and chemical result were found well below the prescribed permissible limits of discharged effluents follows the standard of industrial waste management in Vietnam.
61

Minh-Vien Le, Tuan-Anh Nguyen and T.-Anh-Nga Nguyen, " Modeling and Optimization of the BSCF-Based Single-Chamber Solid Oxide Fuel Cell by Artificial Neural Network and Genetic Algorithm", Journal of Chemistry Volume 2019, Article ID 7828019, 9 pages

https://doi.org/10.1155/2019/7828019

Abstract:
Fuel cells could be a highly effective and eco-friendly technology to transform chemical energy stored in fuel to useful electricity and thus are presently appraised as a standout among the most encouraging advancements for future energy demand. Solid oxide fuel cells (SOFCs) have several advantages over other types of fuel cells, such as the flexibility of fuel used, high energy conversion, and relatively inexpensive catalysts due to high-temperature operation. &e single chambers, wherein the anode and cathode are exposed to the same mixture of fuel, are promising for the portable power application due to the simplified, compact, sealing-free cell structure. &e empirical regression models, such as artificial neural networks (ANNs), can be used as a black-box tool to simulate systems without solving the complicated physical equations merely by utilizing available experimental data. In this study, the performance of the newly proposed BSCF/GDC-based cathode SOFC was modeled using ANNs. &e cell voltage was estimated with cathode preparation temperature, cell operating temperature, and cell current as input parameters by the one-layer feed-forward neural network. In order to acquire the appropriate model, several network structures were tested, and the network was trained by backpropagation algorithms. &e data used during the training, validation, and test are the actual experimental results from our previous study. &e optimum conditions to achieve maximum power of the cell were then determined by the genetic algorithm and the developed ANN.
62

T. N. Tran, N. T. Nguyen. 2018. In Silico Screening for Potent Anti-HCV Compounds with Inhibitory Activities Toward the NS3/4A Protease. International Journal of Peptide Research And Therapeutics 25 (3), 1033-1040

Abstract:
Hepatitis C virus (HCV) infection is a global health problem and the vaccine for this infection is currently unavailable. The HCV NS3 protein is an essential protease for viral polyprotein processing and it requires a NS4A protein as a cofactor for full activity. NS3 and its recombinant protease are currently regarded as a potential target for antiviral drugs. Therefore, finding a specific inhibitor is highly important. In this study, 50,000 peptide bond-containing compounds were screened for the ability against NS3 protease. Three compounds with free energy less than − 10 Kcal/mol were selected, in which, (Z)-2-(3-(3-ethyl-4-oxo-2-thioxothiazolidin-5-ylidene)-2-oxoindolin-1-yl)-N-(3-(trifluoromethyl)phenyl)acetamide showed the highest inhibition against NS3/4A protease. This compound was further investigated in vitro for the ability to inhibit HCV protease and revealed that it competes with substrate to bind into active sites. The kinetic parameter of NS4A–GSGS–NS3 protease with the compound indicated IC50 value of 61.1 µM and Ki of 6.9 µM. Thus, (Z)-2-(3-(3-ethyl-4-oxo-2-thioxothiazolidin-5-ylidene)-2-oxoindolin-1-yl)-N-(3-(trifluoromethyl)phenyl)acetamide could be a potential HCV inhibitor.
63

Bharath Kumar Velmurugan, Chun-Hung Hua, Ming-Hsui Tsai, Chi-Pin Lee, Chia-Min Chung & Ying-Chin Ko, “Combination of celecoxib and calyculin-A inhibits epithelial-mesenchymal transition in human oral cancer cells”, Biotechnic & Histochemistry, (2020) (ISI, IF : 0.948)

https://www.tandfonline.com/doi/full/10.1080/10520295.2019.1700429

Abstract:
Expression of cyclo-oxygenase-2 (COX-2) and protein phosphatase 2A (PP2A) deactivation occurs frequently in oral squamous cell carcinoma (OSCC). We initially assessed COX-2 and PP2A protein expression in OSCC specimens using immunohistochemical (IHC) staining and western blot analysis. We found strong COX-2 and phosphorylated PP2A (p-PP2A) expression in OSCC samples. No significant difference in total PP2A expression was observed between cancer and nontumor tissues. The effect of combining COX-2 inhibitor and celecoxib (CXB) with the PP2A inhibitor, calyculin-A (CLA) on the OSCC cell line, HSC3, was evaluated in vitro. We found that a combination of 1 nM CLA and 50 µM CXB significantly inhibited cell viability, and migration and invasion of HSC3 cells. Western blots for AKT, p-AKT, ERK, p-ERK, E-cadherin, vimentin and β-catenin were conducted after treatment with CXB and/or CLA. Increased E-cadherin and decreased β-catenin expression were found in CXB or CLA treated hsc-3 cells, whereas the combined CXB and CLA treatment showed no difference in E-cadherin or β-catenin expression. Our findings suggest that CLA alone was more effective than CXB alone, but not in the combined drug treatment.
64

Vo Minh Hoang Do, Long Giang Bach, Diem-Huong Nguyen Tran, Van Du Cao, Thi Nhu Quynh Nguyen, Duc Thuan Hoang, Van Cuong Ngo, Dai Hai Nguyen & Thai Thanh Hoang Thi, "Effective Elimination of Charge-associated Toxicity of Low Generation Polyamidoamine Dendrimer Eases Drug Delivery of Oxaliplatin", Biotechnology and Bioprocess Engineering, 25 (2020) 224-234

https://doi.org/10.1007/s12257-019-0047-0

Abstract:
Polyamidoamine (PAMAM) dendrimer is emerging as an effective nanocarrier for delivering anticancer drugs. Still, unmodified PAMAM dendrimer is hardly used in vivo because of unsatisfied drug release, high tendency of interfering with cellular membranes, and rapid clearance by reticuloendothelial system. In this study, low generation polyamidoamine (PAMAM) dendrimer G3.0 is developed and surface modified with methoxypolyethylene glycol (PAMAM G3.0-mPEG) to overcome its limitations. Specifically, PAMAM G3.0 conjugated with mPEG at different ratios are investigated to effectively eliminate its charge-associated toxicity, in which PAMAM G3.0-mPEG- 8 is chosen for oxaliplatin (OX) loading. Results reveal that OX-loaded PAMAM G3.0-mPEG-8 has desirable size, good entrapment efficiency, and sustained release with minimum drug leakage. In addition, Resazurin assay indicates that the toxicity of loaded OX is reduced as compared to free drug but still maintain substantially anticancer activity on HeLa cells, suggesting the potential application of PAMAM G3.0-mPEG-8 for OX delivery in cancer therapy.
65

Abdul, Bakrudeen Ali Ahmed; Alijani, Shirin; Thanabal, Shiyamala Devi; Kim, Se-Kwon "Marine Enzymes Production Tools to the Pharmaceutical Industry", Indian Journal of Geo Marine Sciences 48 (2019) 1656-1666 (ISI, IF: 0.328)

http://nopr.niscair.res.in/handle/123456789/51146

Abstract:
Marine environment contains organisms that make it a profitable natural reservoir, having a tremendous potential to produce functional bio-catalysis, such as amylase, lipase, protease, curtains etc. The enzymes isolated from marine organisms, especially extremophiles, are distinguished by their habitat-related features through bioprospecting processes. These novel features include barophilicity, cold adaptively, salt tolerance and hyperthermo-stability which can alter industrial processes to facilitate mass transfer, energy savings, cost reduction, etc. . This review gives details about the marine enzymes and their, historical discovery followed by isolation processes, introducing seven special marine enzymes with emphasis on their potential applications in chemical, food and pharmaceutical industry.
66

 

Tuong Ha Do, Hoai Bao Truong, Hoang Chinh Nguyen, "Optimization of extraction of phenolic compounds from Ocimum basilicum leaves and evaluation of their antioxidant activity", Pharmaceutical Chemistry Journal, 54, 2, (2020), 162-169 (ISI, IF: 0.538)

https://link.springer.com/article/10.1007%2Fs11094-020-02181-3

Abstract:
Basil (Ocimum basilicum L.) is a potential source of bioactive compounds with various pharmaceutical effects. This study was aimed to extract phenolic compounds from basil leaves and evaluate the antioxidant activity of the extract. Different solvents (methanol, ethanol, acetone, and distilled water) were used to extract the phenolic compounds. Among solvents tested, methanol was the most potent solvent for extraction. The extraction was subsequently optimized using the response surface methodology for maximizing the total phenolic content (TPC). The optimum conditions were found to be the methanol to sample ratio of 44.6 mL/g, methanol concentration of 39%, extraction temperature of 90.7°C, and extraction time of 3.15 h for the maximum TPC of 4.49 mg GAE/g DW. The obtained phenolic-rich extract was then studied for its antioxidant activity. Results showed that the basil leaf extract possessed a high antioxidant capacity with IC50 value of 285.36 μg/mL. This study suggests that basil is a promising source of phenolic compounds and the basil leaf extract is a potential antioxidant agent for pharmaceutical use
67

 

Hoang Chinh Nguyen, Hao-Yeh Lee, Chia-Hung Su, Wei-Jhih Shih, Chien-Chung Chien, "Green process for fatty acid production from soybean oil through microwave-mediated autocatalytic synthesis", Chemical Engineering and Processing-Process Intensification, 147 (2020), 107782 (ISI, IF: 3.731)

https://www.sciencedirect.com/science/article/pii/S0255270119309286

Abstract:
This study developed a new method that involved autocatalytic hydrolysis of soybean oil using microwave irradiation for fatty acid production. To enhance the conversion yield, a surfactant and fatty acid were added to the reaction mixture. The oil hydrolysis using microwave irradiation had 96.6 % conversion yield within a reaction time of 4 h at 195 °C. Furthermore, this study established a model for describing the kinetics of oil hydrolysis performed using traditional heating and microwave irradiation under different reaction conditions. The model exhibited a good fit to the data (R2 of 0.934 to 0.995), demonstrating the reliability of the developed model. The rate constants and pre-exponential factor observed for the oil hydrolysis process using microwave irradiation were higher than those observed for the oil hydrolysis process using traditional heating, indicating that performing oil hydrolysis by using microwave irradiation achieved a reaction more quickly than using traditional heating. These results suggest that autocatalytic oil hydrolysis using microwave irradiation is an ecofriendly and promising method for fatty acid production
68

Quoc Hai Nguyen, Hyeongwoo Kim, Il Tae Kim, Wonchang Choi, Jaehyun Hur, "Few-layer NbSe2@ graphene heterostructures as anodes in lithium-ion half-and full-cell batteries", Chemical Engineering Journal 382 (2020) 122981 (ISI, IF: 10.652).

https://www.sciencedirect.com/science/article/abs/pii/S1385894719323915

Abstract:
A few-layered NbSe2@graphene (FLNG) composite is synthesized via wet ball-milling (WBM) as a new promising anode (wet ball-milled NbSe2@graphene or WBMNG) in lithium-ion half- and full-cell batteries. In this study, we first demonstrate that few-layered graphene (FLG) with low defect density can be prepared from bulk graphite via ball-milling in ethanol. Extending this concept, NbSe2 is introduced as a new 2D additive in WBM to produce a well-defined FLNG composite. FLNG contains NbSe2 particles (~200 nm lateral size and ~7.7 nm thickness (~37 layers)) embedded on a larger FLG (~1 μm lateral size and ~1.7 nm thickness (~5 layers)). The formation of FLNG is based on the solid lubrication in the WBM process that facilitates the exfoliation of 2D materials (NbSe2 and graphite), which leads to the increased surface area, enhanced electrical conductivity, and homogeneous mixing. When applied as an anode in a lithium-ion battery, FLNG (or WBMNG) exhibits excellent electrochemical performances in both WBMNG//Li half-cell and WBMNG//LiFePO4@graphite full-cell batteries. The half-cell displays a reversible discharge capacity as high as ~1000 mAh g−1 (capacity retention of ~88% compared with the initial capacity) at 0.1 A g−1 after 200 cycles and ~700 mAh g−1 at 1 A g−1 after 1000 cycles, with an excellent rate capability (~76% capacity retention at 10 A g−1 compared to the capacity at 0.1 A g−1). Moreover, the practical full-cell delivers a high energy density of ~216 Wh kg−1 after 100 cycles with excellent rate capability.
69

Hoang‐Chinh Nguyen, Kuan‐Hung Lin, Shin‐Lon Ho, Chih‐Ming Chiang, Chi‐Ming Yang, "Enhancing the abiotic stress tolerance of plants: from chemical treatment to biotechnological approaches", Physiologia Plantarum, 164, 4, (2018), 452-466 (ISI, IF: 4.148)

https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12812

Abstract:
Abiotic stresses affect crop plants and cause decreases in plant quality and pro- ductivity. Plants can overcome environmental stresses by activating molecular networks, including signal transduction, stress perception, metabolite produc- tion and expressions of specific stress-related genes. Recent research suggests that chemical priming is a promising field in crop stress management because plants can be primed by chemical agents to increase their tolerance to var- ious environmental stresses. We present a concept to meet this objective and protect plants through priming of existing defense mechanisms avoid- ing manipulation of the genome. In addition, recent developments in plant molecular biology include the discovery of genes related to stress tolerance, including functional genes for protecting cells and regulatory genes for regu- lating stress responses. Therefore, enhancing abiotic stress tolerance using a transgenic approach to transfer these genes into plant genomes has attracted more investigations. Both chemical priming agents and genetic engineering can enhance regulatory and functional genes in plants and increase stress tolerance of plants. This review summarizes the latest findings of chemical priming agents and major achievements in molecular approaches that can potentially enhance the abiotic stress tolerance of plants.
70

Dinh-Chuong Pham, M. A. Shibu, B. Mahalakshmi & Bharath Kumar Velmurugan (2019) Effects of phytochemicals on cellular signaling: reviewing their recent usage approaches, Critical Reviews in Food Science and Nutrition, DOI: 10.1080/10408398.2019.1699014

Abstract:
Most of the previous studies in last three decades report evidence of interactions between the different phytochemicals and the proteins involved in signal transduction pathways using in silico, in vitro, ex vivo, and in vivo analyses. However, extrapolation of these findings for clinical purposes has not been that fruitful. The efficacy of the phytochemicals in vivo studies is limited by parameters such as solubility, metabolic degradation, excretion, etc. Various approaches have now been devised to circumvent these limitations. Recently, chemical modification of the phytochemicals are demonstrated to reduce some of the limitations and improve their efficacy. Similar to traditional medicines several combinatorial phytochemical formulations have shown to be more efficient. Further, phytochemicals have been reported to be even more efficient in the form of nanoparticles. However, systematic evaluation of their efficacy, mode of action in pathway modulation, usage and associated challenges is required to be done. The present review begins with basic understanding of how signaling cascades regulate cellular response and the consequences of their dysregulation further summarizing the developments and problems associated with the dietary phytochemicals and also discuss recent approaches in strengthening these compounds in pharmacological applications. Only context relevant studies have been reviewed. Considering the limitations and scope of the article, authors do not claim inclusion of all the early and recent studies.
71

K.K. Sharker, S. Yusa, Chi Minh Phan, "Micellar formation of cationic surfactants", Heliyon 5 (2019) e02425 (Scopus)

https://linkinghub.elsevier.com/retrieve/pii/S2405844019360852

Abstract:
The micellar structure of six alkyl trimethylammonium halides was studied via conductivity. It was found that the aggregation number increased with the decreasing carbon chain length. Furthermore, Br significantly enhanced the micellar formation over Cl. However, the aggregation number and ionization degree remain similar for both anions. The modelling results validate that the counter-anions affect micellar formation via equilibrium constants, instead of their hydration size. In particular, the association constants between surfactant (both monomer and micelle) and Br are significantly higher than Cl. This is consistent with the qualitative description of hydrated Br in the literature. The experimental and modelling results confirm that micelles are formed via “ion-paring/hydration” structure, instead of the conventional “packing” concept.
72

TM Nguyen, SL Ranamukhaarachchi "Study on the mycelium growth and primordial formation of king oyster mushroom (Pleurotus eryngii) on cardboard and spent coffee ground", Research on Crops 20 (4), (2019) 835-842 (Scopus)

Abstract:
Cultivation of edible mushrooms on urban wastes represents one of the most economically and cost-effective organic recycling processes. An effective tool to identify necessary nutrients for the production of fruiting bodies on the specialty substrates is mycelium growth. Hence, mycelium of king oyster mushroom (Pleurotus eryngii) were experimentally evaluated on cardboard and spent coffee ground (SCG) substrates for the mycelial growth rate, density and primordial formation. Studies were carried out using five different substrate formulations viz., 100% cardboard (TS1), 100% SCG (TS2), 50% cardboard+50% SCG (TS3), 70% cardboard+30% SCG (TS4) and 30% cardboard+70% SCG (TS5). The efficiency of different yeast extract concentrations (i.e. 1, 5, 10, 20, 30 and 50 g/l) as a supplement in potato dextrose agar media (PDA) on P. eryngii growth was also determined. The concentration of 1 g/l of yeast extract showed the best effects on mycelial growth and primordia formation of P. eryngii, while high concentration of yeast extract (5- 50 g/l) did not show any beneficial influence. Combination between cardboard and SCG, especially substrate formulations of 30% cardboard+70% SCG increased mycelial density and number of primordial formations of P. eryngii compared to the substrates of cardboard or SCG alone. The results revealed a potential opportunity for commercial cultivation of edible mushrooms, especially P. eryngii for utilization of different recyclable residues as well as urban generated wastes reduction.
73

Thanh T. Vu, Sojin Song, Hien D.N. Lai, Ngoc Lan Mai, Thuat T. Trinh, Ha T. Do, Dai Phu- Huynh, Anh H. Nguyen, "Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection", Sensing and Bio-Sensing Research, 27(2020) 100322 (ISI, IF: 5.9)

https://www.sciencedirect.com/science/article/pii/S2214180419301540

Abstract:
Gold nanoparticles (AuNPs) have been used to increase the power of electrochemical biosensors. However, densities and pattern distributions of AuNPs colloids have not been determined yet. In this report, we attempt identifying coverable densities of AuNPs on interdigitated electrodes (IDEs) and increasing the sensing signal by using cyclic antigen (CCP)-coated-AuNPs in impedance biosensors. IDEs were covalently functionalized with CCP-coated AuNPs and used for detection of anti-CCP antibody (ACPA), a remarkable biomarker for rheumatoid arthritis. 36% and 82% of ACPA in human commercial serum were captured by a low CCP (300 CCP per 40-nm nanoparticles) and high CCP (17 × 105 CCP per 40-nm nanoparticles) densities of colloids, respectively. Coverage of the colloids on gaps between two digits of IDEs were observed about at 2.7 × 106 AuNPs (30 nm) per 1 μm2 for low signal-to-noise ratio (SNR). The enzyme-catalyzed deposition of silver nanocrystal increased the SNR of the impedance signal up to 7.8 folds of magnitude higher than un-deposited counterparts. Sensitivity and limit of detection were determined at 1.3 ng/mL and 0.12 ng/mL, respectively, at a frequency 900 kHz in the range of 200–2000 kHz. Finally, Principal Component Analysis (PCA) showed the ability of the biosensor to follow variation over time of the number of CCP and therefore also the quantify of ACPA in the samples.
74

Ha Pham Thi Thu, Thu Tra Nguyen, Nguyen Dang Nguyen Thao, Khang Le Minh, and Khang Do Tan, "Evaluate to effects of salt stress on Physico-chemical characteristics in the germination of rice (Oryza sativa L.) in response to methyl salicylate (MeSA)", Biocatalysis and Agricultural Biotechnology, 23, (2020), 101470.

https://doi.org/10.1016/j.bcab.2019.101470

Abstract:
Salinity causes adverse effects to both physiological, morphological and biochemical characteristics that severely inhibit the growth and productivity of rice. Methyl salicylate (MeSA) is one of the common phytochemicals found in plants exposing to abiotic stresses. The study was conducted to evaluate the role of MeSA in rice under vary salt stress conditions. Two rice varieties including HATRI192 (G1) and HATRI62 (G2) were soaked in different concentrations of MeSA (0.1, 0.5, and 1 mM) before placing in saline solutions. The salinity levels included 6, 8, 12, 15 and 17 dS/m of Na+. The shoot height and root length, α-amylase activity, phytic acid content, phenolic, and flavonoid contents were measured during the emerging stage. The results showed that MeSA enhanced the growth of G2 rice seedlings under 6 dS/m and 8 dS/m conditions. The α - amylase activity and phytic content significantly increased in stress condition in some MeSA treatments, obviously in G2 variety. The highest total phenolic and flavonoid contents were found in the treatments 1.0% MeSA +12 dS/m and 0.1% MeSA +15 dS/m, respectively. The finding indicates that MeSA enhanced the physiological and biochemical properties of rice under salt stress condition.
75

Nguyen Thi Lang, Pham Thi Thu Ha, Nguyen Trong Phuoc, And Bui Chi Buu, "Evaluation of Rice Backcross Populations for Salt Tolerance Using Phenotypic Analysis", SABRAO Journal of Breeding and Genetics, 51, 3, (2019) 225-233

Abstract:
Selection of elite rice cultivars through backcross as well as based on quantitative genetics, and interaction of rice cultivars were evaluated by genetic index at the High Agricultural Technology Research Institute (HATRI) for Mekong Delta, Vietnam. A population was developed from a cross between OM10252 (high yielding variety and short duration from Cuu Long Delta Rice Research Institute (CLRRI) as female parent and Pokkali as male parent (a donor in many breeding programs and salinity tolerance related studies from Indian). Analysis of 95 individuals from the BC3F3 population of OM10252 /Pokkali// OM10252, the plant height trait, root length, dry weight of stem, the dried weight of roots had the positive and high correlation with the survival time of rice plants at EC = 0 dS/m, 8 dS/m and 15 dS/m. The line BC3 F4-17 gave good survival at 4‰ condition in different areas. A final comment is that both field management and improved cultural practices were extremely important for sanity in rice. For generations, salt-tolerant lines will continue to grow and will be evaluated in the vegetative and growth stages, and included in breeding programs.
76

Pham Thi Thu Ha, Nguyen Thi Thao Nguyen, Do Thi Nhat Linh, Nguyen Thi Lang, and Bui Chi Buu, "Evaluation Of Rice Germplasm For Yield Traits And Amylose Content Under Drought Stress", SABRAO Journal of Breeding and Genetics, 52, 1, (2020), 64 -74. (Scopus, Q3)

http://sabraojournal.org/wp-content/uploads/2020/04/SABRAO-J-Breed-Genet-521-64-74-HA.pdf

Abstract:
A set of 44 rice cultivars were evaluated for yield traits and amylose content under controlled reproductive stage drought. Drought tolerance indices viz., leaf rolling, leaf drying, and levels of drought tolerance were measured. A total of 38 cultivars had scores of 0–3 for leaf rolling. Forty cultivars showed scores of 0–2 for leaf drying. Twenty cultivars showed drought tolerance scores of 0–3. Six cultivars, namely, IR75499-73-1-B, V3M-92-1, IR75499-21-1-B, V3M-109-2, IR78997-B-16- B-B-B-SB2, and IR78948-B-21-B-B-B, could be selected for drought tolerance, leaf rolling, and leaf drying with drought scores of 0–1. These cultivars were expected to provide higher yield components. The range of yield decline was 10.61–26.06 g/cluster under water stress. Four cultivars showed combined drought tolerance, high yield, and good quality under reproductive-stage drought stress. The drought-tolerant cultivars (V3M-92-1, IR78997-B-16-B-B-B-SB2, IR75499-73-1-B, and IR78948-B-21-B-B-B) identified in this study could be further deployed in rice breeding programs.
77

Duyen Thi Do, Thanh Quynh Trang Le, Nguyen Quoc Khanh Le

Abstract:
Protein S-sulfenylation is one kind of crucial post-translational modifications (PTMs) in which the hydroxyl group covalently binds to the thiol of cysteine. Some recent studies have shown that this modification plays an important role in signaling transduction, transcriptional regulation and apoptosis. To date, the dynamic of sulfenic acids in proteins remains unclear because of its fleeting nature. Identifying S-sulfenylation sites, therefore, could be the key to decipher its mysterious structures and functions, which are important in cell biology and diseases. However, due to the lack of effective methods, scientists in this field tend to be limited in merely a handful of some wet lab techniques that are time-consuming and not cost-effective. Thus, this motivated us to develop an in silico model for detecting S-sulfenylation sites only from protein sequence information. In this study, protein sequences served as natural language sentences comprising biological subwords. The deep neural network was consequentially employed to perform classification. The performance statistics within the independent dataset including sensitivity, specificity, accuracy, Matthews correlation coefficient and area under the curve rates achieved 85.71%, 69.47%, 77.09%, 0.5554 and 0.833, respectively. Our results suggested that the proposed method (fastSulf-DNN) achieved excellent performance in predicting S-sulfenylation sites compared to other well-known tools on a benchmark dataset.
   

Các bài báo trên các tạp chí thuộc danh mục isi/scopus năm học 2018-2019

No Authors
1

Chao Pi-Yu, Meng-Yuan Huang, Wen-Dar Huang, Kuan-Hung Lin, CHEN Shiau-Ying, YANG Chi-Ming, "Study of Chlorophyll-related Compounds from Dietary Spinach in Human Blood", Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46, 2, (2018), 309-316 (ISI, IF: 0.648).

http://notulaebotanicae.ro/index.php/nbha/article/view/10918

Abstract:

Human bioavailability data on chlorophyll (Chl) is very limited. The distribution of Chl-related compounds (CRCs) derived from dietary spinach was investigated in human blood. Eight healthy adults, aged from 21 to 61 year-old, consumed 1.2 kg of just-boiled fresh spinach after an 8-h overnight fast. Before and then 3 h after consuming the spinach, blood samples were taken from each participant. Freeze-dried blood samples were prepared, and 80% acetone was added for grinding. Eight peaks were found in the blood using high-performance liquid chromatography (HPLC), and the main CRCs in the samples were pheophytin (Phe) and pheophorbide (Pho) derivates. Compared to a fasted state, markedly higher levels of blood CRCs were detected in all subjects, except that Pho metabolites were not found in two subjects. No significant differences were seen in most of the peaks between males and females; however, relatively higher CRCs levels were observed in females, particular of Pho derivates. In addition, the blood contained significantly higher levels of Phe in the 36~61-year-old group than in the 21~35-year-old group. These results suggest that the conversion of Chls to CRCs is a rapid process, and Chls obtained by ingestion can be absorbed by the human body.

 

2

Mong-Huai Su, Te-Hua Hsu, Chun-Neng Wang, Kuan-Hung Lin, Ming-Chang Chiang, Ren-Dong Kang, Khiem M Nguyen, Hoang Nguyen, "Genetic Diversity of a Novel Oil Crop, Camellia brevistyla, Revealed by ISSR DNA Markers", Horticultural Science and Technology, 35, 5, (2017), 588-598 (ISI, IF: 0.365).

http://www.kjhst.org/article.php?num=N0130350507

Abstract:

Camellia brevistyla plants are used as food oil in Taiwan. To provide a method for identifying germplasm and assessing the genetic diversity of C. brevistyla, 102 individuals were collected from 29 populations in 11 counties throughout the main island of Taiwan. Inter-simple sequence repeat (ISSR) DNA markers were analyzed and a total of 176 bands were amplified. Of these, 175 bands were found to be polymorphic in these populations. Genetic similarities among populations ranged between 34.1-95.1%. Cluster analysis revealed seven groups of populations plus one outlier (C. kissii ). Five individuals from two populations in the southern region were grouped into cluster V, and six populations from both regions were grouped into cluster VII. The remaining populations from the northern region were separated into different clusters from V and VII. Results of an analysis of molecular variance indicated that the variation within populations (75%) was predominantly greater than variations among counties (18%) and among regions (7%). Overall, the gene flow (Nm = 0.509) estimated from genetic differentiation (Gst = 0.534) suggested that gene flow among regions was limited. Higher genetic diversity (H = 0.317), Shannon’s index (I = 0.477), and gene flow (Nm = 0.538) of populations were found in the northern region compared to the southern region. The DNA sequences of C. brevistyla amplified by two oil-biosynthesis genes showed 99-100% homology with those of C. oleifera. Camellia brevistyla populations are highly differentiated and can serve as a basis for the food oil industry as a germplasm resource.

3

Thanh Mai Nguyen, Nga Thi Thanh Le, Jouni Havukainen, David B Hannaway, "Pesticide use in vegetable production: A survey of Vietnamese farmers’ knowledge", Plant Protection Science, 54, 4, (2018), 203-214 (ISI, IF: 1.076)

https://www.agriculturejournals.cz/web/pps.htm?type=article&id=69_2017-PPS

Abstract:

Concerns about inappropriate storage, application rates, and disposal practices of pesticides prompted this case study of Vietnamese farmers’ knowledge, attitudes, and practices. 128 small-scale vegetable growers in Lam Dong Province were included in field surveys, questionnaires, and interviews. Farmers reported inappropriate mixing of pesticides and disposal methods. Many also reported ill-timed applications posing potential hazards to the human health and environment. Improved training and monitoring of pesticide residues on foodstuffs and in agricultural soils and community water supplies are needed to ensure safe farmer practices. Community-based training and education, jointly funded by local, national, and international agricultural production and food safety groups, would be a cost-effective method of minimising pesticide applications and improving food safety.

4

Nam Nhut Phan, Chih‑Yang Wang, Chien‑Fu Chen, Zhengda Sun, Ming‑Derg Lai, Yen‑Chang Lin, "Voltage-gated calcium channels: Novel targets for cancer therapy", Oncology letters, 14, 2, (2017), 2059-2074 (ISI, IF: 1.664)

https://www.spandidos-publications.com/ol/14/2/2059

Abstract:

Voltage-gated calcium channels (VGCCs) comprise five subtypes: The L type; R type; N type; P/Q type; and T type, which are encoded by α1 subunit genes. Calcium ion channels also have confirmed roles in cellular functions, including mitogenesis, proliferation, differentiation, apoptosis and metastasis. An association between VGCCs, a reduction in proliferation and an increase in apoptosis in prostate cancer cells has also been reported. Therefore, in the present study, the online clinical database Oncomine was used to identify the alterations in the mRNA expression level of VGCCs in 19 cancer subtypes. Overall, VGCC family genes exhibited under expression in numerous types of cancer, including brain, breast, kidney and lung cancers. Notably, the majority of VGCC family members (CACNA1C, CACNA1D, CACNA1A, CACNA1B, CACNA1E, CACNA1H and CACNA1I) exhibited low expression in brain tumors, with mRNA expression levels in the top 1 9% of downregulated gene rankings. A total of 5 VGCC family members (CACNA1A, CACNA1B, CACNA1E, CACNA1G and CACNA1I) were under expressed in breast cancer, with a gene ranking in the top 1 10% of the low expressed genes compared with normal tissue. In kidney and lung cancers, CACNA1S, CACNA1C, CACNA1D, CACNA1A and CACNA1H exhibited low expression, with gene rankings in the top 1 8% of downregulated genes. In conclusion, the present findings may contribute to the development of new cancer treatment approaches by identifying target genes involved in specific types of cancer.

5

Baskaran Rathinasamy, Bharath Kumar Velmurugan, "Role of lncRNAs in the cancer development and progression and their regulation by various phytochemicals", Biomedicine & Pharmacotherapy, 102, (2018), 242-248 (ISI, IF: 3.457)

Link:https://www.sciencedirect.com/science/article/abs/pii/S0753332218305882

Abstract:

Long non coding RNAs (lncRNAs) are involved in modulating the expression of other non-coding RNAs (ncRNA), such as microRNAs, or target proteins through the epigenetic, transcriptional, or post-transcriptional regulations. Genomic mutations in cancer reside inside regions that do not code for proteins and these regions are often transcribed into long non coding RNAs (lncRNAs). Emerging evidences have revealed an intense involvement of lncRNAs in the cancer development and progression. Recently, emerging evidences have depicted that the phytochemicals interact with lncRNAs to modulate their activities. Such findings are highly important for the identification of therapeutic strategies against diseases that are particularly associated with an aberrant lncRNA signaling. This review aims at deciphering the role of lncRNAs in the cancer development and progression, and their regulation by various phytochemicals.

6

Hoang Chinh Nguyen, Tran Quynh Mong Nhu, Phung Van Dung, Nguyen Dinh Hieu, Tran Trong Tuan, Phan Xuan Huyen, Dieu-Hien Truong, "Evaluation of changes in the growth and chemical constituents of Anoectochilus formosanus Hayata grown under hydroponic conditions", BioTechnologia, 99, 4, (2018), 375-383 (Scopus)

Link: http://www.biotechnologia-journal.org/Evaluation-of-changes-in-the-growth-and-chemical-constituents-of-Anoectochilus-formosanus-Hayata-grown-under-hydroponic-conditions,85,34258,0,1.html

Abstract:

Anoectochilus formosanus Hayata is an important medicinal plant with various pharmaceutical properties. In this study, A. formosanus plants were hydroponically cultivated in different nutrient solutions to achieve enhanced biomass and secondary metabolites production. Three months-old A. formosanus plants were grown for 8 weeks under controlled environment in plastic pots containing Murashige and Skoog (MS), Nitrophoska Foliar (NF), Hydro Green (HG), or Hydro Bee (HB) media. Among 4 nutrient solutions tested, HB was the most efficient medium for the plant growth with the highest fresh weight (FW, 2.56 g/plant) and dry weight (DW, 0.18 g/plant) values. The results of phytochemical screening showed the presence of alkaloids, flavonoids, terpenoids, glycosides, and steroids in the extracts of A. formosanus cultivated in HB, HG, and MS medium. The level of these compounds was significantly different in plants cultivated in tested media. The highest alkaloids (34.87 μg/g DW) and terpenoids (56.43 μg/g DW) contents were obtained on HG medium, whereas flavonoids were present in highest amounts (90.13 μg QE/g DW) in plants grown in NF medium. On the other hand, HB medium stimulated the production of the highest glycoside (64.33 μg/g DW) and steroids (22.83 μg/g DW) levels. The antioxidant activity of the extracts was also tested using the 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity assay and the results demonstrated strong antioxidant activities of A. formosanus extracts with IC50 of 136.19 to 248.85 μg/ml. Concluding, the hydroponic-cultivation of A. formosanus is a promising way for obtaining highly valuable compounds for pharmaceutical and nutraceutical industries.

7

Roshan Khadka, Zane Zondaka, Arko Kesküla, Mahdi Safaei Khorram, Tran Thien Khanh, Tarmo Tamm, Jadranka Travas‐Sejdic, Rudolf Kiefer, "Influence of solvent on linear polypyrrole–polyethylene oxide actuators", Journal of Applied Polymer Science, 135, 43, (2018), 46831 (ISI, IF: 1.901)

    Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/app.46831

Abstract:

Ionic conductivity of polypyrrole (PPy) doped with dodecylbenzenesulfonate (DBS) was improved by loading of 10 wt % of polyethylene oxide (PEO). The linear actuation properties of PPy–PEO/DBS films were investigated and compared in polar (aqueous) and aprotic (propylene carbonate) solvents keeping the concentration of lithium bis(trifluoromethane)sulfonimide (LiTFSI) electrolyte the same. The results were compared to those of pristine PPy/DBS films. The actuation direction changed from pure cation‐driven in the aqueous electrolyte (LiTFSI(aq)) to anion‐driven in propylene carbonate electrolyte (LiTFSI(PC)). At the same time, the electro‐chemo‐mechanical deformation revealed that the strain of PPy–PEO/DBS increased from 5% in LiTFSI(aq) to 20% in LiTFSI(PC). The effect of solvent on the ionic conductivity was investigated using electrochemical impedance spectroscopy, showing a 27% increase in charge transfer kinetics and an increase in the electronic conductivity, resulting in significant increase in the strain rate, when propylene carbonate electrolyte was used.

8

Zane Zondaka, Alo Kivilo, Sunjai Nakshatharan, Kadi-Anne Küppar, Urmas Johanson, Tarmo Tamm, Rudolf Kiefer, "Carbide-derived carbon and poly-3, 4-ethylenedioxythiphene composite laminate: linear and bending actuation", Synthetic Metals, 245, (2018), 67-73 (ISI, IF: 2.526)
Link: https://www.sciencedirect.com/science/article/abs/pii/S0379677918302443

Abstract:
Carbide-derived carbon is a well-known material for ionic electroactive systems like sensors and actuators. Typically, a gold foil or other current collector is applied on top of either side to enhance the conductivity of such laminates. Here, metal foil is replaced by an electrochemically deposited layer of poly-3,4-ethylenedioxythiophene (PEDOT). While lower in conductivity, PEDOT has the advantages of lowered risk of delamination and being electro-chemo-mechanically active itself. Such laminates were tested in both linear actuation mode in lithium bis(trifluoromethane) sulfonamide propylene carbonate solution (LiTFSI-PC) under isotonic and isometric electro-chemo-mechanical deformation measurements (ECMD), and in bending mode in air using a room-temperature ionic liquid as the electrolyte. The exchanged charge as well as the actuation response of the laminate was dominated by the PEDOT layer, overriding even the actuation direction of the carbon-based layers. The stress of all investigated samples was found in similar range of 30 kPa. The materials were characterized by scanning electron microscopy (SEM), FTIR and energy-dispersive X-Ray spectroscopy (EDX).
9

Bharath Kumar Velmurugan, Wei-Hsiang Chang, Chia-Min Chung, Chung-Min Yeh, Chien-Hsun Lee, Kun-Tu Yeh, Shu-Hui Lin, "DDR2 overexpression in oral squamous cell carcinoma is associated to lymph node metastasis", Cancer Biomarkers, 22, 4, (2018), 747-753 (ISI, IF: 2.392)

Link: https://content.iospress.com/articles/cancer-biomarkers/cbm181302

Abstract:

BACKGROUND: Discoidin domain receptors (DDRs), a collagen receptor tyrosine kinase, play a major role in cancer progression. DDR2 has been suggested as a prognostic marker in several cancer types; however, the correlation between DDR2 expression and clinical outcome of oral cancer patients in Taiwan population has not been investigated. MATERIALS AND METHODS: In the present study we sought to determine the clinical significance of Discoidin Domain Receptor Tyrosine Kinase 2 (DDR2) expression in oral squamous cell carcinoma (OSCC) patients. We examined DDR2 expression in OSCC specimens by immunohistochemistry and then we analyzed the association of DDR2 expression with clinicopathological factors in OSCC. RESULTS: We divided 254 OSCC cases into two groups based on DDR2 expression levels and compared with several clinicopathological factors and their overall survival. The group with high DDR2 expression had significantly higher frequencies of lymph node metastasis (P= 0.0094) and AJCC stage (P= 0.0058) compared to the group with low DDR2 expression. Furthermore, the lymph node metastasis oral cancer patients with high DDR2 expression had low survival rate than low DDR2 group (P= 0.0458). CONCLUSIONS: Our data indicate that DDR2 is a potent biomarker that can be used as an effective therapeutic target for treating OSCC patients with lymph node metastasis.

 

10

Shang-Lun Chiang, Bharath Kumar Velmurugan, Chia-Min Chung, Shu-Hui Lin, Zhi-Hong Wang, Chun-Hung Hua, Ming-Hsui Tsai, Tzer-Min Kuo, Kun-Tu Yeh, Pei-Ying Chang, "Preventive effect of celecoxib use against cancer progression and occurrence of oral squamous cell carcinoma", Scientific reports, 7, 1, (2017), 6235  (ISI, IF: 4.122)

Link: https://www.nature.com/articles/s41598-017-06673-3

Abstract:

Overexpression of cyclooxygenase-2 in oral cancer increases lymph node metastasis and is associated with a poor prognosis. The potential of celecoxib (CXB) use is reported in cancer treatment by inhibiting proliferation through apoptosis, but the effects on the epithelial-mesenchymal transition (EMT) and cancer cell mobility remain unclear. We performed a preclinical study and population-based study to evaluate CXB use in the prevention of oral cancer progression and occurrence. The in-vitro findings showed that CXB is involved in the inhibition of EMT and cell mobility through blocking transcription factors (Slug, Snail and ZEB1), cytoplasmic mediators (focal adhesion kinase (FAK), vimentin and β-catenin), cell adhesion molecules (cadherins and integrins), and surface receptors (AMFR and EGFR). The murine xenograft model showed a 65% inhibition in tumour growth after a 5-week treatment of CXB compared to placebo. Xenograft tumours in placebo-treated mice displayed a well-to-moderate/moderate differentiated SCC grade, while those from CXB-treated mice were well differentiated. The expression levels of membrane EGFR, and nuclear FAK, Slug and ZEB1 were decreased in the xenograft tumours of CXB-treated mice. A retrospective cohort study showed that increasing the daily dose and medication time of CXB was associated with oral cancer prevention. The findings provide an alternative prevention strategy for oral cancer development with CXB use.

 

11

Su‐Ying Wen, Bharath Kumar Velmurugan, Cecilia Hsuan Day, Chia‐Yao Shen, Li‐Chin Chun, Yi‐Chieh Tsai, Yueh‐Min Lin, Ray‐Jade Chen, Chia‐Hua Kuo, Chih‐Yang Huang, "High density lipoprotein (HDL) reverses palmitic acid induced energy metabolism imbalance by switching CD36 and GLUT4 signaling pathways in cardiomyocyte", Journal of cellular physiology, 232, 11, (2017), 3020-3029 (ISI, IF: 3.923)

Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.26007

Abstract:

In our previous study palmitic acid (PA) induced lipotoxicity and switches energy metabolism from CD36 to GLUT4 in H9c2 cells. Low level of high density lipoprotein (HDL) is an independent risk factor for cardiac hypertrophy. Therefore, we in the present study investigated whether HDL can reverse PA induced lipotoxicity in H9c2 cardiomyoblast cells. In this study, we treated H9c2 cells with PA to create a hyperlipidemia model in vitro and analyzed for CD36 and GLUT4 metabolic pathway proteins. CD36 metabolic pathway proteins (phospho‐AMPK, SIRT1, PGC1α, PPARα, CPT1β, and CD36) were decreased by high PA (150 and 200 μg/μl) concentration. Interestingly, expression of GLUT4 metabolic pathway proteins (p‐PI3K and pAKT) were increased at low concentration (50 μg/μl) and decreased at high PA concentration. Whereas, phospho‐PKCζ, GLUT4 and PDH proteins expression was increased in a dose dependent manner. PA treated H9c2 cells were treated with HDL and analyzed for cell viability. Results showed that HDL treatment induced cell proliferation efficiency in PA treated cells. In addition, HDL reversed the metabolic effects of PA: CD36 translocation was increased and reduced GLUT4 translocation, but HDL treatment significantly increased CD36 metabolic pathway proteins and reduced GLUT4 pathway proteins. Rat neonatal cardiomyocytes showed similar results. In conclusion, HDL reversed palmatic acid‐induced lipotoxicity and energy metabolism imbalance in H9c2 cardiomyoblast cells and in neonatal rat cardiomyocyte cells.

12

Peiying Pai, Bharath Kumar Velmurugan, Chia-Hua Kuo, Chung-Yi Yen, Tsung-Jung Ho, Yueh-Min Lin, Yu-Feng Chen, Chao-Hung Lai, Cecilia Hsuan Day, Chih-Yang Huang, "17β-Estradiol and/or estrogen receptor alpha blocks isoproterenol-induced calcium accumulation and hypertrophy via GSK3β/PP2A/NFAT3/ANP pathway", Molecular and cellular biochemistry, 434, 1-2, (2017), 181-195 (ISI, IF: 2.561)

Link: https://link.springer.com/article/10.1007/s11010-017-3048-3

Abstract:

The present study was aimed to investigate the protective effects of 17β-estradiol (E2) and estrogen receptor α (ERα) on isoproterenol (ISO)-treated H9c2 cardiomyoblast cells. In the present study, we treated H9c2 cells with ISO, a β-adrenergic receptor agonist, to induce myocardiac hypertrophy. Pre-administration of E2 or ERα (induced by doxycycline) and E2 plus ERα significantly prevented ISO-induced increase of cell size and cytosolic calcium accumulation, accompanied with increased mRNA of atrial natriuretic peptide and brain natriuretic peptide. However, ICI-ERs antagonist, and melatonin, a specific inhibitor for ERα, reversed the cardioprotective effects, suggesting that E2 action was mediated through ERα. Further evidences showed that E2 and ERα increased the protein level of GSK3β and protein phosphatase 2a inhibitor 2 (I2-PP2A), which subsequently enhanced the activation of I2-PP2A by disrupting PP2A activity and maintains normal calcium outflow. Collectively, E2 and ERα inhibited hypertrophy by preventing cytosol calcium accumulation and by inhibiting the association between PP2A with Na+–Ca2+ exchanger via GSK3β and I2-PP2A activation.

 

13

Rathinasamy Baskaran, Bharath Kumar Velmurugan, "Protein phosphatase 2A as therapeutic targets in various disease models", Life sciences, 210, 1, (2018), 40-46 (ISI, IF: 3.234)

Link: https://www.sciencedirect.com/science/article/abs/pii/S0024320518305241

 

Abstract:
There are a large number of signalling pathways responsible for transmitting information within the cell. Although cellular signalling is thought to be majorly governed by protein kinases ‘cascade effects’; their antagonists protein phosphatases also play a crucial dual role in signal transduction. By dephosphorylating the proteins involved in signalling pathways, phosphatases may lead to their activation and sometimes they may terminate a signal generated by kinases activity. Due to counterbalancing the function of phosphorylation, the protein phosphatases are very important to signal transduction processes and thus the control of phosphatase activity is as significant as kinases, in the regulation of a plethora of cellular processes. In general, the protein phosphatases are comprised of a catalytic subunit with one or more regulatory and/or targeting subunits associated with it. The Protein Phosphatase 2A (PP2A), a member of serine/threonine phosphatases family, is ubiquitously expressed a remarkably conserved enzyme in the cell. Its catalytic activity has been highly regulated and may have enormous therapeutic potential which is still untapped. It has specificities for a number of substrates which witnessed its involvement in various signalling modules of cell cycle regulation, cell morphology and development. Thus it can be an appropriate target for studying different diseases associated with abnormal signal transduction pathways such as neurodegenerative diseases and malignancies. This review will focus on the structure and regulatory pathways of PP2A. The de-regulation of PP2A in some specific pathology such as Cancer, Heart diseases, Neurodegenerative disorders and Diabetes will also be touched upon.
14

Nancy Maurya, Bharath Kumar Velmurugan, "Therapeutic applications of cannabinoids", Chemico-biological interactions, 293, (2018), 77-88 (ISI, IF: 3.296)

Link: https://www.sciencedirect.com/science/article/pii/S0009279718307373

Abstract:

The psychoactive property of cannabinoids is well known and there has been a continuous controversy regarding the usage of these compounds for therapeutic purposes all over the world. Their use for medical and research purposes are restricted in various countries. However, their utility as medications should not be overshadowed by its negative physiological activities. This review article is focused on the therapeutic potential and applications of phytocannabinoids and endocannabinoids. We further highlights their mode of action, overall effects on physiology, various in vitro and in vivo studies that have been done so far and the extent to which these compounds can be useful in different disease conditions such as cancer, Alzheimer's disease, multiple sclerosis, pain, inflammation, glaucoma and many others. Thus, this work is an attempt to make the readers understand the positive implications of these compounds and indicates the significant developments of utilizing cannabinoids as therapeutic agents.

15

Tran Thien Khanh, Rong-Jer Lee, Paul A Kilmartin, Md Asaduzzaman Khan, Mahdi Safaei Khorram, Tarmo Tamm, Rudolf Kiefer, "Actuation increase in polypyrrole bilayer by photo-activated dopants", Synthetic Metals, 246, (2018), 57-63 (ISI, IF: 2.526)

Link: https://www.sciencedirect.com/science/article/abs/pii/S0379677918304016

Abstract:

A new methodology to increase the polypyrrole (PPy) bilayer actuation displacement is presented, based on photo-activated dopants generating secondary charges. Two dopants, dodecyl benzenesulfonate (DBS) and the photo-active dopant 2-diazo-1-naphthol-5-sulfonic acid (DNSA), were compared in this study. PPy/DBS, PPy/DBS-DNSA and PPy/DNSA bilayers on polyethylene terephthalate were formed and their actuation properties in aqueous electrolyte were investigated applying cyclic voltammetry and square wave potential steps. Exposure to solar irradiation increased PPy/DBS-DNSA and PPy/DNSA bilayer bending displacements by two and three times, respectively, accompanied by increased charge density during the reversible redox cycles. UV–vis and Fourier transform infrared (FTIR) measurements were also performed to follow the photo reaction of the photo-active dopants.

16

M Shanmugavadivu, Bharath Kumar Velmurugan, "Pharmalogical activities of antroquinonol-Mini review", Chemico-biological interactions, 297, 5, (2018), 8-15 (ISI, IF: 3.296)

Link: https://www.sciencedirect.com/science/article/pii/S000927971831216X

Abstract:
Antrodia camphorata is an expensive mushroom that grows on the inner cavity of an endangered native tree of Taiwan namely Cinnamomum kanehirai Hayata. It is used as a traditional medicine in Taiwan and has several health benefits including free radical scavenging, anti-inflammatory, antimicrobial, hepatoprotective, neuroprotective, antidiabetic, and free radical-induced DNA damage protecting activities. Antroquinonol is a tetrahydro ubiquinone derivative found predominately in the mycelium of Antrodia camphorata, and is characterized by numerous biological and pharmacological activities. Several studies have revealed potential anticancer effects of antroquinonol in various carcinogenic models. Moreover, a phase II clinical trial is ongoing in the US and Taiwan to treat the lung cancer patients with this active compound. The present review aims at depicting a detailed view of the synthetic procedures of antroquinonol as well as deciphering its potential health benefits with a special emphasis on anticancer properties.
17

Lin Kuan-Hung, WU Chun-Wei, Yu-Sen Chang, "Applying Dickson Quality Index, Chlorophyll Fluorescence, and Leaf Area Index for Assessing Plant Quality of Pentas lanceolata", Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47, 1, (2018), 169-176 (ISI, IF: 0.648)

Link: http://notulaebotanicae.ro/index.php/nbha/article/view/11312

Abstract:
Plant quality greatly relates to the seedling vigor (SV), survival and growth of plants after transplantation. The objective of this study was to use the nondestructive measurements of chlorophyll fluorescence (ChlF) and leaf area index (LAI) as SV indices for star cluster (Pentas lanceolata). Plants were grown in potting soil under nature sunlight for 90 d. A total of 13 morphological and physiological parameters were selected for measurements. Among them, root growth potential (RGP) was the best predictor for SV in all tested plants. Plants were separated into 5 RGP groups based on the number of new roots, and remaining parameters were also separated into those same levels. The trends and rates of increase from levels 1 to 5 in Dickson quality index (DQI), LAI, total dry mass, and ChlF were all similar to the RGP index. Although RGP and DQI are frequently used as indices for SV, these measurements are time-consuming and require sample destruction. Consistent and strongly high correlations were observed among DQI, LAI, and ChlF, demonstrating the applicability of these indices for measuring SV in star cluster. The measurements of LAI and ChlF were predicted using multiple variables from validation datasets, and showed novel and useful parameters for examining the SV of star cluster.
18

Dinh-Chuong Pham, Yu-Chuan Chang, Shian-Ren Lin, Yuh-Ming Fuh, May-Jywan Tsai, Ching-Feng Weng, "FAK and S6K1 inhibitor, Neferine, dually induces autophagy and apoptosis in human neuroblastoma cells", Molecules, 23, 12, (2018), 3110 (ISI, IF: 3.098)

Link: https://www.mdpi.com/1420-3049/23/12/3110

Abstract:
Human neuroblastoma cancer is the most typical extracranial solid tumor. Yet, new remedial treatment therapies are demanded to overcome its sluggish survival rate. Neferine, isolated from the lotus embryos, inhibits the proliferation of various cancer cells. This study aimed to evaluate the anti-cancer activity of neferine in IMR32 human neuroblastoma cells and to expose the concealable molecular mechanisms. IMR32 cells were treated with different concentrations of neferine, followed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess cell viability. In an effort to determine the molecular mechanisms in neferine-incubated IMR32 cells, cell cycle arrest, cell migration, and focal adhesion kinase (FAK), the 70-kDa ribosomal S6 kinase 1 (S6K1), poly (ADP-ribose) polymerase (PARP), caspase-3, Beclin-1, and microtubule-associated protein 1A/1B-light chain 3 (LC3) protein expressions were investigated. Neferine strongly disrupted the neuroblastoma cell growth via induction of G2/M phase arrest. Furthermore, neferine provoked autophagy and apoptosis in IMR32 cells, confirmed by p-FAK, and p-S6K1 reduction, LC3-II accumulation, Beclin-1 overexpression, and cleaved caspase-3/PARP improvement. Finally, neferine markedly retarded cell migration of neuroblastoma cancer cells. As a result, our findings for the first time showed an explicit anti-cancer effect of neferine in IMR32 cells, suggesting that neferine might be a potential candidate against human neuroblastoma cells to improve clinical outcomes with further in vivo investigation.
19

Zane Zondaka, Madis Harjo, Mahdi Safaei Khorram, Pejman Rasti, Tarmo Tamm, Rudolf Kiefer, "Polypyrrole/carbide-derived carbon composite in organic electrolyte: Characterization as a linear actuator", Reactive and Functional Polymers, 131, (2018), 414-419 (ISI, IF: 2.975)

Link: https://www.sciencedirect.com/science/article/abs/pii/S1381514818307910

Abstract:
Polypyrrole (PPy) doped with dodecylbenzenesulphonate (DBS) (PPy/DBS) was polymerized with the addition of phosphotungstic acid (PTA), thus, incorporating multicharged phosphotungstate anions (PT) to give PPy/DBS-PT films. With carbide-derived carbon (CDC) particles included, the obtained films contained CDC-PT, DBS and PT anions forming PPy/DBS-CDC-PT (PPy/CDC). Our goal was to test the applicability of the material for actuation in an organic electrolyte. The material properties of PPy/CDC films, such as conductivity, charging/discharging and actuation as strain and strain rate were significantly changed in comparison to PPy/DBS-PT films. FTIR (Fourier transform infrared) and EDX (energy dispersive X-ray) spectroscopy revealed that CDC-PT is incorporated in the PPy films and the SEM (scanning electron microscopy) images showed a more porous film with CDC particles packed into PPy. Electro-chemo-mechanical deformation studies (ECMD) revealed that PPy/CDC films had anion-dominated actuation resulting in nearly 6 times higher strain, 2 times higher force, higher strain rates, and 7 times higher conductivity than PPy/DBS-PT films, which had mixed ion transport and rather poor strain and stress behavior. Thus, only one of the two materials - PPy/CDC – could have some practical use in this type of electrolyte solutions.
20

P Muralidhar Reddy, Nancy Maurya, Bharath Kumar Velmurugan, "Medicinal Use of Synthetic Cannabinoids—a Mini Review", Current Pharmacology Reports, 5, 1, (2019), 1-13 (Scopus)

Link: https://link.springer.com/article/10.1007/s40495-018-0165-y

Abstract:

Purpose of Review

This review gives an overview of the medicinal uses of synthetic cannabinoids and other related aspects on the basis of recent as well as earlier studies that the authors considered relevant to the context and scope of the review.

Recent Findings

Synthetic cannabinoids are laboratory synthesized products eliciting effects way more than their natural counterparts. These compounds are more potent in generating intoxicating effects and are also difficult to be detected in conventional screening tests. Their clinical side effects are also more pronounced than natural cannabinoids, and their antidotes are also not known. However, they are also therapeutically found to be very effective in many health conditions, as these act by interacting with almost ubiquitously distributed cannabinoid receptors (CB1 and CB2) in the human body and by other mechanisms also that do not involve these receptors.

Summary

All the issues related to their appropriate dosage, mode of action, acute and chronic effects in vivo, interaction with other drugs, their metabolism, etc. need much research to be done so that it will be easier to predict their different aspects in human subjects in more appropriate way. Further, development of strict legislation and regulation is required to be done so that their abuse can be curbed, and toxic effects can be reduced, but medicinal benefits and usage can be enhanced.

21

VH Giang Phan, Huu Thuy Trang Duong, Thavasyappan Thambi, Thanh Loc Nguyen, Md Hasan Turabee, Yue Yin, Seong Han Kim, Jaeyun Kim, Ji Hoon Jeong, Doo Sung Lee, "Modularly engineered injectable hybrid hydrogels based on protein-polymer network as potent immunologic adjuvant in vivo", Biomaterials, 195, (2019), 100-110 (ISI, IF: 8.806)

Link: https://www.sciencedirect.com/science/article/pii/S014296121830869X

Abstract:
Lymphoid organs, which are populated by dendritic cells (DCs), are highly specialized tissues and provide an ideal microenvironment for T-cell priming. However, intramuscular or subcutaneous delivery of vaccine to DCs, a subset of antigen-presenting cells, has failed to stimulate optimal immune response for effective vaccination and need for adjuvants to induce immune response. To address this issue, we developed an in situ-forming injectable hybrid hydrogel that spontaneously assemble into microporous network upon subcutaneous administration, which provide a cellular niche to host immune cells, including DCs. In situ-forming injectable hybrid hydrogelators, composed of protein-polymer conjugates, formed a hydrogel depot at the close proximity to the dermis, resulting in a rapid migration of immune cells to the hydrogel boundary and infiltration to the microporous network. The biocompatibility of the watery microporous network allows recruitment of DCs without a DC enhancement factor, which was significantly higher than that of traditional hydrogel releasing chemoattractants, granulocyte-macrophage colony-stimulating factor. Owing to the sustained degradation of microporous hydrogel network, DNA vaccine release can be sustained, and the recruitment of DCs and their homing to lymph node can be modulated. Furthermore, immunization of a vaccine encoding amyloid-β fusion proteinbearing microporous network induced a robust antigen-specific immune response in vivo and strong recall immune response was exhibited due to immunogenic memory. These hybrid hydrogels can be administered in a minimally invasive manner using hypodermic needle, bypassing the need for cytokine or DC enhancement factor and provide niche to host immune cells. These findings highlight the potential of hybrid hydrogels that may serve as a simple, yet multifunctional, platform for DNA vaccine delivery to modulate immune response.
22

Nguyen Quang Khuyen, Jose G Martinez, Friedrich Kaasik, Tarmo Tamm, Toribio F Otero, Rudolf Kiefer, "Solvent effects on carbide-derived-carbon trilayer bending actuators", Synthetic Metals, 247, (2019), 170-176 (ISI, IF: 2.526)

Link: https://www.sciencedirect.com/science/article/pii/S0379677918305861

Abstract:
Abstract:
Bending actuators were prepared by depositing carbide-derived carbon, a material typical for electric double layer capacitors, on both sides of poly-vinylidenefluoride membranes, forming CDC-trilayers. Their actuation properties were studied using 0.5 M solutions of lithium perchlorate (LiClO4) in different solvents: water, ethylene glycol, acetonitrile, and propylene carbonate. The goal of this work was to study the actuation mechanism, charging-discharging properties in these solvents, as well as to establish the optimal solvent for maximum bending displacement. It was found that while the actuation direction was the same for all solvents, pointing to similar mechanism, the exchanged charge and the displacement differed considerably. Moreover, the highest specific capacitance found in ethylene glycol did not bring along the highest displacement, neither was the highest exchanged charge of propylene carbonate the most efficient option, the acetonitrile was the clear winner. The available electrochemical windows for the reversible charging also differed considerably.
23

Thai Thanh Hoang Thi, Thi Nhu Quynh Nguyen, Duc Thuan Hoang, Van Cuong Ngo, Dai Hai Nguyen, "Functionalized mesoporous silica nanoparticles and biomedical applications", Materials Science and Engineering: C, 99, (2019), 631-656 (ISI, IF: 5.080)

Link: https://www.sciencedirect.com/science/article/pii/S0928493117343461

Abstract:
Since the first report in early 1990s, mesoporous silica nanoparticles (MSNs) have progressively attracted the attention of scientists due to their potential applications in physic, energy storage, imaging, and especially in biomedical engineering. Owning the unique physiochemical properties, such as highly porosity, large surface area and pore volume, functionalizable, tunable pore and particle sizes and biocompatibility, and high loading cavity, MSNs offer efficient encapsulation and then controlled release, and in some cases, intracellular delivery of bioactive molecules for biomedical applications. During the last decade, functionalized MSNs that show respond upon the surrounding stimulus changes, such as temperature, pH, redox, light, ultrasound, magnetic or electric fields, enzyme, redox, ROS, glucose, and ATP, or their combinations, have continuously revolutionized their potential applications in biomedical engineering. Therefore, this review focuses on discussion the recent fabrication of functionalized MSNs and their potential applications in drug delivery, therapeutic treatments, diagnostic imaging, and biocatalyst. In addition, some potential clinical applications and challenges will also be discussed.
24

Hoang Chinh Nguyen, Chia-Hung Su, Yuan-Kun Yu, Dinh Thi My Huong, "Sugarcane bagasse as a novel carbon source for heterotrophic cultivation of oleaginous microalga Schizochytrium sp", Industrial crops and products, 121, (2018), 99-105 (ISI, IF: 3.849)

Link: https://www.sciencedirect.com/science/article/pii/S0926669018304163

Abstract:
Oleaginous microalgae are known as promising oil producers, which accumulate high amount of lipids from various carbon substrates. This study first investigated sugarcane bagasse hydrolysate as a cheap carbon source for the production of biomass and lipid by Schizochytrium sp. The sugarcane bagasse was pretreated with alkali followed by phosphoric acid to remove lignin and enhance xylose production, respectively. The enzymatic hydrolysis of the pretreated sugarcane bagasse by cellulase was subsequently optimized. A maximum glucose yield of 95.77% was obtained at an enzyme loading of 0.3 mL/g with a hydrolysis reaction time of 48 h. The sugarcane bagasse hydrolysate containing glucose and xylose was subsequently used as a substrate for cultivating Schizochytrium sp. Results showed that sugarcane bagasse hydrolysate performed better than refined glucose for cell growth and lipid accumulation. The maximum biomass (10.45 g/L) and lipid content (45.15%) were achieved by growing Schizochytrium sp. in a medium containing 40 g/L glucose in sugarcane bagasse hydrolysate for 72 h. Sugarcane bagasse hydrolysate also resulted in higher levels of polyunsaturated fatty acid and docosahexaenoic acid than did refined glucose. This study suggests that sugarcane bagasse hydrolysate is a low-cost and effective carbon source for microalgal biomass and lipid production.
25

Hoang Chinh Nguyen, Shih-Hsiang Liang, Shang-Sian Chen, Chia-Hung Su, Jhih-Huei Lin, Chien-Chung Chien, "Enzymatic production of biodiesel from insect fat using methyl acetate as an acyl acceptor: optimization by using response surface methodology", Energy Conversion and Management, 158, (2018), 168-175 (ISI, IF: 6.377)

Link: https://www.sciencedirect.com/science/article/pii/S019689041731227X

Abstract:
Black soldier fly larvae (BSFL) are oleaginous insects that can assimilate organic waste for fat accumulation, and thus serve as an alternative feedstock for biodiesel production. In lipase-catalyzed transesterification, enzymes are deactivated by excess methanol. To address this obstacle, methyl acetate is suggested as an alternative acyl acceptor to methanol. In this study, methyl acetate was first used in the enzymatic production of biodiesel with BSFL as a triglyceride source. The interesterification of BSFL fat with methyl acetate was catalyzed using Novozym 435 as an efficient immobilized lipase. Response surface methodology was used to optimize the reaction and establish a reliable mathematical model for prediction. A maximum biodiesel yield of 96.97% was reached at a reaction time of 12 h, molar ratio of methyl acetate to fat of 14.64: 1, enzyme loading of 17.58%, and temperature of 39.5 °C. Under these optimal reaction conditions, Novozym 435 could be reused for up to 20 cycles without loss in enzyme activity. The properties of BSFL biodiesel were also investigated and all met the European standard EN 14214. This study indicates that the enzymatic interesterification of BSFL fat with methyl acetate is a promising and ecofriendly method for green fuel production.

 

26

Long‐Bin Jeng, Bharath Kumar Velmurugan, Hsi‐Hsien Hsu, Su‐Ying Wen, Chia‐Yao Shen, Chih‐Hao Lin, Yueh‐Min Lin, Ray‐Jade Chen, Wei‐Wen Kuo, Chih‐Yang Huang, "Fenofibrate induced PPAR alpha expression was attenuated by oestrogen receptor alpha overexpression in Hep3B cells", Environmental toxicology, 33, 2, (2018), 234-247 (ISI, IF: 2.491)

Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.22511

Abstract:
The physiological regulation of Oestrogen receptor α (ERα) and peroxisome proliferator‐activated receptor alpha (PPARα) in Hepatocellular carcinoma (HCC) remains unknown. The present study we first treat the cells with fenofibrate and further investigated the possible mechanisms of 17β‐estradiol (E2) and/or ERα on regulating PPARα expression. We also found higher PPARα expression in the tumor area than adjacent areas and subsequently compared PPARα expression in four different hepatic cancer cell lines. Hep3B cells were found to express more PPARα than the other cell lines. Using the PPARα agonist fenofibrate, we found that fenofibrate increased Hep3B cell proliferation efficiency by increasing cell cycle proteins, such as cyclin D1 and PCNA, and inhibiting p27 and caspase 3 expressions. Next, we performed transient transfections and immuno‐precipitation studies using the pTRE2/ERα plasmid to evaluate the interaction between ERα and PPARα. ERα interacted directly with PPARα and negatively regulated its function. Moreover, in Tet‐on ERα over‐expressed Hep3B cells, E2 treatment inhibited PPARα, its downstream gene acyl‐CoA oxidase (ACO), cyclin D1 and PCNA expression and further increased p27 and caspase 3 expressions. However, over‐expressed ERα plus 17‐β‐estradiol (10−8 M) reversed the fenofibrate effect and induced apoptosis, which was blocked in ICI/melatonin/fenofibrate‐treated cells. This study illustrates that PPARα expression and function were negatively regulated by ERα expression in Hep3B cells.
27

Bharath Kumar Velmurugan, Lohanathan Bharathi Priya, Paramasivan Poornima, Li‐Jen Lee, Rathinasamy Baskaran, "Biomaterial aided differentiation and maturation of induced pluripotent stem cells", Journal of cellular physiology, 234, 6, (2019), 8443-8454 (ISI, IF: 3.923)

Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27769

Abstract:
Engineering/reprogramming differentiated adult somatic cells to gain the ability to differentiate into any type of cell lineage are called as induced pluripotent stem cells (iPSCs). Offering unlimited self‐renewal and differentiation potential, these iPSC are aspired to meet the growing demands in the field of regenerative medicine, tissue engineering, disease modeling, nanotechnology, and drug discovery. Biomaterial fabrication with the rapid evolution of technology increased their versatility and utility in regenerative medicine and tissue engineering, revolutionizing the stem cell biology research with the property to guide the process of proliferation, differentiation, and morphogenesis. Combining traditional culture platforms of iPSC with biomaterials aids to overcome the limitations associated with derivation, proliferation, and maturation, thereby could improve the clinical translation of iPSC. The present review discusses in brief about the reprogramming techniques for the derivation iPSC and details on several biomaterial guided differentiation of iPSC to different cell types with specific relevance to tissue engineering/regenerative medicine.
28

Long‐Bin Jeng, Bharath Kumar Velmurugan, Ming‐Cheng Chen, Hsi‐Hsien Hsu, Tsung‐Jung Ho, Cecilia‐Hsuan Day, Yueh‐Min Lin, V Vijaya Padma, Chuan‐Chou Tu, Chih‐Yang Huang, "Fisetin mediated apoptotic cell death in parental and Oxaliplatin/irinotecan resistant colorectal cancer cells in vitro and in vivo", Journal of cellular physiology, 233, 9, (2018), 7134-7142 (ISI, IF: 3.923)

Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.26532

Abstract:
Irinotecan (CPT11) and Oxaliplatin have been used in combination with fluorouracil and leucovorin for treating colorectal cancer. However, the efficacy of these drugs is reduced due to various side effects and drug resistance. Fisetin, a hydroxyflavone possess anti‐proliferative, anti‐cancer, anti‐inflammatory, and antioxidant activity against various types of cancers. Apart from that, fisetin has been shown to induce cytotoxic effects when combined with other known chemotherapeutic drugs. In this study, we aimed to investigate whether Fisetin was capable of sensitizing both Irinotecan and Oxaliplatin resistance colon cancer cells and explored the possible signaling pathways involved using In vitro and In vivo models. The results showed that Fisetin treatment effectively inhibited cell viability and apoptosis of CPT11‐LoVo cells than Oxaliplatin (OR) and parental LoVo cancer cells. Western blot assays suggested that apoptosis was induced by fisetin administration, promoting Caspase‐8, and Cytochrome‐C expressions possibly by inhibiting aberrant activation of IGF1R and AKT proteins. Furthermore, fisetin inhibited tumor growth in athymic nude mouse xenograft model. Overall, our results provided a basis for Fisetin as a promising agent to treat parental as well as chemoresistance colon cancer.
29

Bharath Kumar Velmurugan, Chi‐Ru Chan, Ching‐Feng Weng, "Innate‐immune responses of tilapia (Oreochromis mossambicus) exposure to acute cold stress", Journal of cellular physiology, (2019) (ISI, IF: 3.923)

Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28270

 

Abstract:
In this present study, Oreochromis mossambicus tilapia were transferred to cold water at 12°C for various time intervals (1, 4, 8, 24, and 48 hr) and its innate immune response was analyzed by studying cellular and humoral parameters. In vivo, alternative complement pathway activity in blood plasma was rapidly increased at 1 hr of cold water (12°C) exposure. Lysozyme activity and cortisol levels of plasma were increased at 4 and 1 hr, respectively. Surprisingly, only plasma cortisol levels remained unchanged through 24 hr of cold water transfer. Phagocytic ability, phagocytic capacity, and respiratory burst (RB) activity of head kidney (HK) leukocytes and splenocytes showed no any significant changes. In peripheral blood leukocytes, phagocytic capacity, and RB activity were increased at 24 hr of cold water exposure. The expressions of genes involved innate immunity in splenocytes and HK leukocytes of tilapia cold water exposure were analyzed, messenger RNA (mRNA) expressions of HSP70, HSP90, and immunoglobulin M failed to change upon exposure to cold stress. Major histocompatibility complex‐I and II mRNAs were significantly increased in tilapia splenocytes at 1 hr of cold water transferred. Whereas myxovirus (Mx) expression was increased in splenocytes and HK leukocytes of tilapia after 1 hr of cold water exposed. Our result reveals that the exposure of tilapia to acute cold stress condition significantly enhances plasma acid phosphatase activity and both phagocytic capacity and RB activity. Furthermore, cold stress significantly stimulates Mx gene expression in splenocytes and HK leukocytes.
30

Tran Thien Khanh, Arko Kesküla, Zane Zondaka, Madis Harjo, Alo Kivilo, Mahdi Safaei Khorram, Tarmo Tamm, Rudolf Kiefer, "Role of polymerization temperature on the performance of polypyrrole/dodecylbenzenesulphonate linear actuators", Synthetic Metals, 247, (2019), 53-58 (ISI, IF: 2.526)

Link: https://www.sciencedirect.com/science/article/pii/S0379677918305381

Abstract:
Polypyrrole doped with dodecylbenzenesulphonate (PPy/DBS) free-standing films were electropolymerized in sodium perchlorate propylene carbonate solution at different temperatures, observing increasing conductivity with decreasing synthesis temperature. Our goal in this study was to evaluate how the linear actuation of PPy depends on the polymerization temperature. The anion driven actuation of materials synthesized at lower temperatures changed to mainly cation active for PPy/DBS films polymerized at 20 °C; the latter films also showed the highest diffusion coefficients. Scanning electron microscopy did show that the surface roughness of the films increased with increasing synthesis temperature, as expected. Isometric and isotonic electro-chemo mechanical deformation (ECMD) measurements were performed with combined electrochemical techniques (cyclic voltammetry and chronoamperometry), revealing wide differences in the actuation behavior.
31

C-Y Huang, BK Velmurugan, M-C Chen, CH Day, W-S Chien, VV Padma, H-C Wu, T-H Lin, H-H Hsu, C-H Shen, "KHC-4 inhibits β-catenin expression in prostate cancer cells", Biotechnic & Histochemistry, (2019), 1-7 (ISI, IF: 1.074)

Link: https://www.tandfonline.com/doi/abs/10.1080/10520295.2019.1574026

Abstract:
KHC-4 is a 2-phenyl-4-quinolone analogue that exhibits anticancer activity. Aberrant activation of β-catenin signaling contributes to prostate cancer development and progression. Therefore, targeting β-catenin expression could be a useful approach to treating prostate cancer. We found that KHC-4 can inhibit β-catenin expression and its signaling pathway in DU145 prostate cancer cells. Treatment with KHC-4 decreased total β-catenin expression and concomitantly decreased β-catenin levels in both the cytoplasm and nucleus of cells. KHC-4 treatment also inhibited β-catenin expression and that of its target proteins, PI3K, AKT, GSK3β and TBX3. We monitored the stability of β-catenin with the proteasomal inhibitor, MG132, in DU145 cells and found that MG132 reversed KHC-4-induced proteasomal β-catenin degradation. We verified CDK1/β-catenin expression in KHC-4 treated DU145 cells. We found that roscovitine treatment reversed cell proliferation by arresting the cell cycle at the G2/M phase and β-catenin expression caused by KHC-4 treatment. We suggest that KHC-4 inhibits β-catenin signaling in DU145 prostate cancer cells.
32

Thai Minh Duy Le, Huu Thuy Trang Duong, Thavasyappan Thambi, VH Giang Phan, Ji Hoon Jeong, Doo Sung Lee, "Bioinspired pH-and Temperature-Responsive Injectable Adhesive Hydrogels with Polyplexes Promotes Skin Wound Healing", Biomacromolecules, 19, 8, (2018), 3536-3548 (ISI, IF: 5.738)

Link: https://pubs.acs.org/doi/abs/10.1021/acs.biomac.8b00819

Abstract:
Despite great potential, the delivery of genetic materials into cells or tissues of interest remains challenging owing to their susceptibility to nuclease degradation, lack of permeability to the cell membrane, and short in vivo half-life, which severely restrict their widespread use in therapeutics. To surmount these shortcomings, we developed a bioinspired in situ-forming pH- and temperature-sensitive injectable hydrogel depot that could control the delivery of DNA-bearing polyplexes for versatile biomedical applications. A series of multiblock copolymer, comprised of water-soluble poly(ethylene glycol) (PEG) and pH- and temperature-responsive poly(sulfamethazine ester urethane) (PSMEU), has been synthesized as in situ-forming injectable hydrogelators. The free-flowing PEG–PSMEU copolymer sols at high pH and room temperature (pH 8.5, 23 °C) were transformed to stable gel at the body condition (pH 7.4, 37 °C). Physical and mechanical properties of hydrogels, including their degradation rate and viscosity, are elegantly controlled by varying the composition of urethane ester units. Subcutaneous administration of free-flowing PEG–PSMEU copolymer sols to the dorsal region of Sprague–Dawley rats instantly formed hydrogel depot. The degradation of the hydrogel depot was slow at the beginning and found to be bioresorbable after two months. Cationic protein or DNA-bearing polyplex-loaded PEG–PSMEU copolymer sols formed stable gel and controlled its release over 10 days in vivo. Owing to the presence of urethane linkages, the PEG–PSMEU possesses excellent adhesion strength to wide range of surfaces including glass, plastic, and fresh organs. More importantly, the hydrogels effectively adhered on human skin and peeled easily without eliciting an inflammatory response. Subcutaneous implantation of PEG–PSMEU copolymer sols effectively sealed the ruptured skin, which accelerated the wound healing process as observed by the skin appendage morphogenesis. The bioinspired in situ-forming pH- and temperature-sensitive injectable adhesive hydrogel may provide a promising platform for myriad biomedical applications as controlled delivery vehicle, adhesive, and tissue regeneration.
33

Huu Thuy Trang Duong, Yue Yin, Thavasyappan Thambi, Thanh Loc Nguyen, VH Giang Phan, Min Sang Lee, Jung Eun Lee, Jaeyun Kim, Ji Hoon Jeong, Doo Sung Lee, "Smart vaccine delivery based on microneedle arrays decorated with ultra-pH-responsive copolymers for cancer immunotherapy", Biomaterials, 185, (2018), 13-24 (ISI, IF: 8.806)

Link: https://www.sciencedirect.com/science/article/pii/S0142961218306392

Abstract:
Despite the tremendous potential of DNA-based cancer vaccines, their efficacious delivery to antigen presenting cells to stimulate both humoral and cellular response remains a major challenge. Although electroporation-based transfection has improved performance, an optimal strategy for safe and pain-free vaccination technique remains elusive. Herein, we report a smart DNA vaccine delivery system in which nanoengineered DNA vaccine was laden on microneedles (MNs) assembled with layer-by-layer coating of ultra-pH-responsive OSM-(PEG-PAEU) and immunostimulatory adjuvant poly(I:C), a synthetic double stranded RNA. Transcutaneous application of MN patches onto the mice skin perforate the stratum corneum with minimal cell damage; subsequent disassembly at the immune-cell-rich epidermis/dermis allows the release of adjuvants and DNA vaccines, owing to the ultra-sharp pH-responsive nature of OSM-(PEG-PAEU). The released adjuvant and DNA vaccine can enhance dendritic cell maturation and induce type I interferons, and thereby produce antigen-specific antibody that can achieve the antibody-dependent cell-mediated cytotoxicity (ADCC) and CD8+ T cell to kill cancer cells. Strikingly, transcutaneous application of smart vaccine formulation in mice elicited 3-fold greater frequencies of Anti-OVA IgG1 serum antibody and 3-fold excess of cytotoxic CD8+ T cell than soluble DNA vaccine formulation. As a consequence, the formulation rejected the murine B16/OVA melanoma tumors in C57BL/6 mice through the synergistic activation of antigen-specific ADCC and cytotoxic CD8+ T cells. The maneuvered use of vaccine and adjuvant poly(I:C) in MNs induces humoral and cellular immunity, which provides a promising vaccine technology that shows improved efficacy, compliance, and safety.
34

Siti Norayu Idris, Abdul Bakrudeen Ali Ahmed and Rosna Mat Taha, “Sucrose enhanced stigmasterol production in callus cultures of Wedelia biflora (L.) D.C.”, Philippine Agricultural Scientist, 2018, 101, 3, 251-260 (ISI, IF: 0.27).

https://pas.cafs.uplb.edu.ph/category/2018/

Abstract:
Callus culture method was used to extract stigmasterol in mass amount. The current study aims to enhance the production of stigmasterol by manipulation of sucrose concentrations in Murashige and Skoog (MS) media. A combination of 2 mg NAA L-1 (an auxin) and 2.5 mg 6-benzylaminopurine (BAP) L-1 (a cytokinin) in MS medium was the best concentration to stimulate callus formation from stem explants of W. biflora with the fastest response (10 d), the highest biomass of callus (55 mg dry weight) and the best callus characteristic (green and compact). Application of 4% (w/v) sucrose in MS medium enhanced callus formation along with callus biomass and stigmasterol production (88 mg g-1) compared with the control (58.3 mg g-1) and in vivo grown stem (53.4 mg g-1). Production of stigmasterol from W. biflora with reduced production time and cost is now possible with the methods obtained from the current study.
35

A.B.A. Ahmed, J. Magesh, S.N. Idris, E.N. Mubarak and R.M. Taha, “Chemical Constituents and Antimicrobial Activity of Essential Oils from Micropropagation and Field-Grown Plants ofWedelia biflora [L.]”, Journal of essential oil bearing plants, 2018, 21, 6, 1712-1724 (ISI: IF 0.72)
https://www.tandfonline.com/doi/abs/10.1080/0972060X.2018.1538819

Abstract:
An efficient regeneration protocol through axillary and inter nodal explants was developed from Wedelia biflora. Explants were placed on MS medium supplemented with different treatments of cytokinins, 6-benzyladenine (BA) and kinetin (KIN) for adventitious shoot production. The highest multiple shoots (95 %; 26.4 shoots/ explant) and elongation (2.75 cm shoot length), was obtained in 1.0 BA mg L-1 in axillary node explants. The shoot was transferred to half-strength MS medium supplemented with 2.0 mg L-1 IBA showed the best root formation (85 %; 22.2 roots/shoot; 2.65 cm root length; propagate leaf d.w 1.42 g L-1 and root d.w 1.10 g L-1) and plantlets were successfully acclimatized in the green house. The compositions of essential oils were studied in in vitro and in vivo leaf explants and to determine their absolute and relative concentrations using GC-MS and GC-FID, respectively. In vitro and in vivo variations in composition among the essential oils were detected. Sesquiterpene and monoterpene are major components in the oil of in vitro and in vivo leaves of W. biflora. Essential oil quantification showed α-pinene (44.03 %); verbenone (15.37 %); δ-cadinene (5.48 %) of in vivo leaves; whereas α-pinene (5.02 %); verbenone (1.40 %) and δ-cadinene (3.13 %) of in vitroleave respectively. The in vitro and in vivo essential oils were analyzed by bacterial and fungal organism through disc diffusion and minimum inhibitor concentration (MIC) methods. The results were compared with reference drugs ciprofloxacin and nystatin respectively. In vitro developed plants exhibited higher sesquiterpene contents and antimicrobial activity than field grown plant.
36

Bharath Kumar Velmurugan, Baskaran Rathinasamy, Bharathi Priya Lohanathan, Varadharajan Thiyagarajan and Ching-Feng Weng, “Neuroprotective Role of Phytochemicals”, Molecules, 2018, 23 (10), 2485 (ISI: IF: 3.060)
https://www.mdpi.com/1420-3049/23/10/2485

Abstract:
Neurodegenerative diseases are normally distinguished as disorders with loss of neurons. Various compounds are being tested to treat neurodegenerative diseases (NDs) but they possess solitary symptomatic advantages with numerous side effects. Accumulative studies have been conducted to validate the benefit of phytochemicals to treat neurodegenerative diseases including Alzheimer’s disease (AD) and Parkinson’s disease (PD). In this present review we explored the potential efficacy of phytochemicals such as epigallocatechin-3-galate, berberin, curcumin, resveratrol, quercetin and limonoids against the most common NDs, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). The beneficial potentials of these phytochemicals have been demonstrated by evidence-based but more extensive investigation needs to be conducted for reducing the progression of AD and PD.
37

M.S. Khorram, G. Zhang, A. Fatemi, R. Kiefer, K. Maddah, M. Baqar, Md P. Zakaria and G. Li, “Impact of biochar and compost amendment on soil quality, growth and yield of a replanted apple orchard in a 4-year field study”, Journal of the Science of Food and Agriculture, 2019, 99, 1862-1869 (ISI, h index 121, IF 2.42). 
https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9380

Abstract:
BACKGROUND
Numerous studies have addressed the positive effects of organic amendments on soil and plant productivity under short‐term field studies. However, to date, few studies have been conducted on the effects of organic amendment on the orchards where high nutrient bioavailability is required. This study deals with the effects of biochar and compost on soil quality, growth and yield of a replanted apple orchard in the northeast of Iran.
RESULTS
Biochar+compost application resulted in 37% and 300% higher soil total organic carbon and available phosphorus content, respectively, during the first 3 years of experimentation compared to control. Similarly, trunk diameter and shoot number of apple trees increased 23–26% by the end of the first year. Nevertheless, there were no significant changes in fruitfulness, fruit weight or starch pattern index as productivity indices.
CONCLUSION
Biochar and compost were beneficial in improving soil quality, mainly by increasing soil nutrient content and decreasing soil bulk density, and in increasing plant growth at early growth stages of apple orchards. However, they failed to enhance overall yield and fruit quality, most likely due to their limited ability to suppress apple replant disease.
38

I. Põldsalu, U. Johanson, T. Tamm, A. Punning, F. Greco, A.-L. Peikolainen, R. Kiefer, A. Aabloo, “Mechanical and electro-mechanical properties of EAP actuators with inkjet printed electrodes”, Synthetic Metals, 2018, 246, 122–127 (ISI, h index 132, IF 2.9). 
https://www.sciencedirect.com/science/article/abs/pii/S0379677918304338

Abstract:
Electrically conductive polymer (CP) based ionic electromechanically active polymer composites (IEAP-s) are attractive as bending and linear actuators in compliant and miniature devices due to low operating voltage. Ink-jet printing is a promising technology for fabrication of microscale CP-based IEAP-s with customized shapes and geometries. The current study investigates tailoring of the mechanical and electromechanical properties of the actuators by controlled growth of ink-jet printed poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS) based electrodes on commercial poly(vinylidene fluoride) (PVdF) membranes. In parallel with PEDOT:PSS, hybrid actuators with ink-jet printed PEDOT:PSS and activated carbon aerogel electrodes were investigated. Cumulative growth of electrodes with each deposited layer was achieved in the case of both electrode materials. The strain, blocking force and capacitance of the actuators were in linear correlation with the thickness of the electrodes. Simple method of control encourages implementation of ink-jet-printing technology for manufacturing of IEAP micro-actuators with desired mechanical and electromechanical properties.
39

M.Tania, J. Shawon, K. Saif, R. Kiefer, M. Safaei Khorram, Md. A. Halim and Md. A. Khan, “Cordycepin Downregulates Cdk-2 to Interfere with Cell Cycle and Increases Apoptosis by Generating ROS in Cervical Cancer Cells: in vitro and in silico Study”, Current Cancer Drug Targets, 2019, 19, 152-159 (ISI, H index 85, IF: 2.28).
https://www.ingentaconnect.com/contentone/ben/ccdt/2019/00000019/00000002/art00007

Abstract:
Background: Cordycepin is a small molecule from medicinal mushroom Cordyceps, which has been reported for anticancer properties.
Objective: In this study, we have focused on the investigation of cordycepin effect on cervical cancer cells with further clarification of possible molecular mechanism. 
Method: We have used cell viability and cell counting assay for cytotoxic effect of cordycepin, flow cytometric assay of apoptosis and cell cycle, and quantitative PCR (qPCR) and Western blotting for the determination of target gene expression. Molecular docking and Molecular dynamics simulation were used for in silico analysis of cordycepin affinity to target protein(s). 
Results: Treatment of cordycepin controlled SiHa and HeLa cervical cancer cell growth, increased the rate of their apoptosis, and interfered with cell cycle, specifically elongated S-phase. qPCR results indicated that there was a downregulation of cell cycle proteins CDK-2, CYCLIN-A2 and CYCLIN-E1 in mRNA level by cordycepin treatment but no significant change was observed in pro-apoptotic or antiapoptotic proteins. The intracellular reactive oxygen species (ROS) level in cordycepin treated cells was increased significantly, implying that apoptosis might be induced by ROS. Western blot analysis confirmed significant decrease of Cdk-2 and mild decrease of Cyclin-E1 and Cyclin-A2 by cordycepin, which might be responsible for regulating cell cycle. Molecular docking indicated high binding affinity of cordycepin against Cdk-2. Molecular dynamics simulation further confirmed that the docked pose of cordycepin-Cdk-2 complex remained within the binding pocket for 10 ns. 
Conclusion: Our study suggests that cordycepin is effective against cervical cancer cells, and regulating cell cycle via cell cycle proteins, especially downregulating Cdk-2, and inducing apoptosis by generating ROS are among the mechanisms of anticancer activities of cordycepin.
40

R. Kiefer, R. Khadka, J. Travas-Sejdic, “Poly(ethylene oxide) in polypyrrole doped dodecylbenzenesulfonate: characterisation and linear actuation”, International Journal of Nanotechnology, 2018, 15, 689-694 (ISI, H index 32, IF 0.56).  
https://www.inderscienceonline.com/doi/abs/10.1504/IJNT.2018.098435

Abstract:
We show improvement in linear actuation properties of polypyrrole-doped dodecylbenzenesulfonate (PPy/DBS) by incorporating varying amounts of polyethylene oxide (5 wt % or 15 wt %) into PPy during electroploymerisation (PPy/DBS-PEO). Knowing that pristine PPy/DBS linear actuators in aqueous electrolytes are driven by cation ingress during reduction, the actuation properties are studied in isotonic (length change) mode of electro-chemo-mechanical-deformation (ECMD) measurements. Scanning electronic microscopy (SEM) and energy dispersive X-ray (EDX) spectroscopy revealed that PEO is incorporated in PPy/DBS films. ECMD studies revealed a three-fold improvement in electronic conductivity for the PPyDBS-PEO 15% film that also demonstrated improved strain of 7% in comparison to the strain of PPy/DBS film of 1.9%.
41

Z. Zondaka, T. Tamm, A. Aabloo, R. Kiefer, "Solvent change in polymerization influence linear actuation of polypyrrole carbide-derived carbon films ," Proceedings SPIE 10594, Electroactive Polymer Actuators and Devices (EAPAD) XX, 105942H (27 March 2018) (conference scopus, h index 151, IF 0.56) 
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10594/105942H/Solvent-change-in-polymerization-influence-linear-actuation-of-polypyrrole-carbide/10.1117/12.2300146.short

Abstract:
For actuator applications, conducting polymers are typically deposited at lower temperatures e.g. -20°C, in order to obtain more regular, smooth and ordered films with fewer defects. Clearly, standard aqueous electrolyte solutions cannot be used at these temperatures, and some organic solvents cannot be used, as not all salts are soluble in these. A wellknown approach is to use ethylene glycol: water mixtures (often 50:50 wt%, PPy/CDC(EG:W). The goal of this work is to analyze the role of water in the solution: to compare the linear actuation properties of conducting polymer hybrid films PPy/CDC(EG) polymerized from just ethylene glycol solution and that with 50% water, both at -20°C. Cyclic voltammetry and chronoamperometric electro-chemo-mechanical-deformation (ECMD) measurements were performed to study the linear actuation properties in lithium bis(trifluoromethylsulfonyl)imide in aqueous solution (LiTFSI-aq). Both conductivity and linear actuation strain were found dependent on the solvent in the polymerization solution with ethylene glycol: water the clearly superior solvent choice.
42

A. Kivilo, Z. Zondaka, S. Nakshatharan, K.-A. Küppar, T. Tamm, R. Kiefer, "Poly-3,4-ethylenedixoythiophene on carbide-derived carbon trilayer: combined linear actuation characterization", Proc. SPIE 10594, Electroactive Polymer Actuators and Devices (EAPAD) XX, 105942L (27 March 2018) (conference scopus, h index 151, IF 0.56) 
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10594/105942L/Poly-34-ethylenedixoythiophene-on-carbide-derived-carbon-trilayer--combined/10.1117/12.2300133.short

Abstract:
Carbide-derived carbon (CDC) based actuators have been typically formulated as trilayer systems and applied in bending displacement. In this work, we want to demonstrate that CDC deposited on glass fiber fabric (CDC-TL) with an additional poly-3.4-ethylenedioxythiophene (PEDOT) layer electropolymerized on top forming a polylayer (expressed as CDC-PEDOT-TL) can be used as a linear actuator. Isotonic and isometric electro-chemo-mechanical deformation (ECMD) measurements in lithium bis(trifluoromethane) sulfonamide propylene carbonate (LiTFSI-PC) were performed, revealing that the CDC expansion at discharging can be found in CDC-PEDOT-TL (main expansion at oxidation of 0.5% strain) in same extent of 0.24 %. The stress found in similar values of 30 kPa for both system. Besides the nearly 5times better conductivity of CDC-PEDOT-TL, the charge density reduce nearly half in comparison to CDC-TL.
43

Dieu-Hien Truong, Dinh Hieu Nguyen, Nhat Thuy Anh Ta, Anh Vo Bui, Tuong Ha Do and Hoang Chinh Nguyen, “Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia”, Journal of Food Quality, 2019, 8178294 (ISI: IF: 1,767).
https://www.hindawi.com/journals/jfq/2019/8178294/

Abstract:
Severinia buxifolia (Rutaceae) is a promising source of bioactive compounds since it has been traditionally used for the treatment of various diseases. The present study aimed at evaluating the impact of different solvents on extraction yields, phytochemical constituents and antioxidants, and in vitro anti-inflammatory activities of S. buxifolia. The results showed that the used solvents took an important role in the yield of extraction, the content of chemical components, and the tested biological activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w). The extract obtained from methanol exhibited high capacity of antioxidant (IC50value of 16.99 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 28.86 μg/mL; antiproteinase activity: IC50 = 414.29 μg/mL; and membrane stabilization: IC50 = 319 μg/mL). The antioxidant activity of the S. buxifolia extract was found to be 3-fold higher than ascorbic acid, and the anti-inflammatory activity of S. buxifolia extract was comparable to aspirin. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy.
44

Nguyen Chi Thanh, Boonchai Wichitsathian, Chatpet Yossapol, Watcharapol Wonglertarak and Borano Te, “Improvement of aqueous solution coexisting with arsenite and arsenate using iron mixed porous clay pellets in batch and fixed-bed column studies”, Water Science and Technology: Water Supply, 2019, ws2019069 (ISI: IF: 0.674).
https://iwaponline.com/ws/article-abstract/doi/10.2166/ws.2019.069/67109/Improvement-of-aqueous-solution-coexisting-with?redirectedFrom=fulltext

Abstract:
Arsenic-polluted water is a global concern and puts millions of people at risk of developing cancer. The improvement of aqueous solution coexisting with arsenite and arsenate using iron mixed porous clay pellets was investigated in batch and fixed-bed column systems. Batch studies showed that the removal rate occurred in two main phases with an equilibrium time of 52 h. The pseudo-second-order model well described the experimental data. Isotherm data were well fitted by the Langmuir–Freundlich model. The removal efficiency was significantly reduced in alkaline solution and the presence of phosphate ions. The column study revealed that the breakthrough time and saturation time increased with lower feeding flow rate, higher bed height, and lower initial adsorbate concentration. The Thomas model provided good performance for predicting the column experimental data.
45

Nagarajan Ganesan, Rathinasamy Baskaran, Bharath Kumar Velmurugan and Nguyen Chi Thanh, “Antrodia cinnamomea—An updated minireview of its bioactive components and biological activity”, Journal of Food Biochemistry, 2019, 43, e12936 (ISI: IF: 1.552).
https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12936

Abstract:
Antrodia cinnamomea or Antrodia camphorata is a distinctive mushroom of Taiwan, which is being used as a traditional medicine to treat various health‐related conditions. More than 78 compounds have been identified in A. cinnamomea. Large numbers of phytochemical studies have been carried out in A. cinnamomea due to the high amount of terpenoids. Besides that, the extracts and active components of A. cinnamomea were reported to have various biological activities including hepatoprotective, antihypertensive, antihyperlipidemic, anti‐inflammatory, antioxidant, antitumor, and immunomodulatory activities. In this review article, we have summarized the recent findings of A. cinnamomea and its molecular mechanisms of action in various disease models.
46

Minh-Khiem Nguyen, Kuan-Hung Lin, Li-Ru Chen, Tung-Chuan Hsiung and Yukio Ozaki, “Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr.”, Journal of the Faculty of Agriculture, Kyushu University, 2018, 63, 2, 231–240 (ISI: IF: 0.351). 
https://catalog.lib.kyushu-u.ac.jp/opac_download_md/1955387/p231.pdf

Abstract:
An efficient mass propagation method for Bletilla formosana (Hayata) Schltr. was successfully established through direct shoot organogenesis. Multiple shoots were induced from protocorm explants on half– strength Murashige and Skoog (1/2–MS) basal medium containing 2 mg/L N–phenyl–N’–1,2,3–thiadiazol–5– yl urea (TDZ) and 0.5 mg/L 1–naphthaleneacetic acid (NAA) with the highest shoot formation rate of 100% and a maximum average shoot number of 5.2. The application of either NAA or 2,4–dichlorophenoxy (2,4–D) significantly induced root induction from shoots, and 2 mg/L NAA provided the highest root formation rate of 100% and a maximum number of roots (4.4 per shoot). Supplementation with kinetin and benzylaminopurine (BAP) of 0.5~2.0 mg/L in 1/2–MS basal medium significantly promoted plantlet growth. To optimize the in vitro development of plantlets, 1/2–MS basal medium with modified 1/4–strength nitrogen content, and 20 g/L sucrose was used. Well–developed plantlets were successfully acclimatized in a greenhouse with over a 90% survival rate. This effective protocol of in vitro plant regeneration through direct shoot organogenesis can be utilized for the mass propagation and germplasm conservation of B. formosana.
47

Kuo-Chan Hung, Ngoc Tuan Nguyen, Yu-Ling Sun and Shir-Ly Huang, “Bio-Fenton reaction involved in the cleavage of the ethoxylate chain of nonionic surfactants by dihydrolipoamide dehydrogenase from Pseudomonas nitroreducens TX1”, Scientific Reports, 2019, 9, 6827 (ISI: IF: 4.122)
https://www.nature.com/articles/s41598-019-43266-8

Abstract:
Bacteria in the environment play a major role in the degradation of widely used man-made recalcitrant organic compounds. Pseudomonas nitroreducens TX1 is of special interest because of its high efficiency to remove nonionic ethoxylated surfactants. In this study, a novel approach was demonstrated by a bacterial enzyme involved in the formation of radicals to attack ethoxylated surfactants. The dihydrolipoamide dehydrogenase was purified from the crude extract of strain TX1 by using octylphenol polyethoxylate (OPEOn) as substrate. The extent of removal of OPEOs during the degradation process was conducted by purified recombinant enzyme from E. coli BL21 (DE3) in the presence of the excess of metal mixtures (Mn2+, Mg2+, Zn2+, and Cu2+). The metabolites and the degradation rates were analyzed and determined by liquid chromatography-mass spectrometry. The enzyme was demonstrated to form Fenton reagent in the presence of an excess of metals. Under this in vitro condition, it was shown to be able to shorten the ethoxylate chains of OPEOn. After 2 hours of reaction, the products obtained from the degradation experiment revealed a prominent ion peak at m/z = 493.3, namely the ethoxylate chain unit is 6 (OPEO6) compared to OPEO9 (m/z = 625.3), the main undegraded surfactant in the no enzyme control. It revealed that the concentration of OPEO15 and OPEO9 decreased by 90% and 40% after 4 hours, respectively. The disappearance rates for the OPEOn homologs correlated to the length of the exothylate chains, suggesting it is not a specific enzymatic reaction which cleaves one unit by unit from the end of the ethoxylate chain. The results indicate the diverse and novel strategy by bacteria to catabolize organic compounds by using existing housekeeping enzyme(s).
48

Le Quoc Bao, Vu Hoang Giang Phan and Nguyen Quang Khuyen, “Investigating effects of nano cerium oxide reinforcement on mechanical properties of composite based on natural rubber”, AIP Conference Proceedings, 2018, 1954, 1, 10.1063/1.5033391 (conference scopus, h index 54) 
https://aip.scitation.org/doi/10.1063/1.5033391

Abstract:
Polymer nanocomposites that based on combination of nanomaterials (such as nanoparticles, nanotubes, nanorods, nanofibers, and nanosheets) and polymeric matrices are receiving great attention in research and application. However, separate and homogenous dispersion rather than aggregates of nanoparticles into matrices meet big difficulty due to large interaction between nanoparticles. The poor dispersion leads to low properties of nanocomposites. In this study, we find out the appropriate method to separately disperse cerium oxides (CeO2) nanoparticles into natural rubber, aiming to increase mechanical properties of natural rubber. The SEM images were used to evaluate the dispersion of nano CeO2 in natural rubber matrix. The mechanical properties of nanocomposites were measured after vulcanization to investigate effects of nano CeO2amount on prepared composite. The findings exhibited that the addition of CeO2 by dispersion of nano CeO2 in water via ultrasonication before mixing with rubber latex, significantly increase modulus, tear and wear resistance of natural rubber. 
49

Hoang Chinh Nguyen, Dinh Thi My Huong, Horng-Yi Juan, Chia-Hung Su and Chien-Chung Chien, “Liquid Lipase-Catalyzed Esterification of Oleic Acid with Methanol for Biodiesel Production in the Presence of Superabsorbent Polymer: Optimization by Using Response Surface Methodology”, Energies, 2018, 11, 5, 1085 (ISI: IF 2.676)
https://www.mdpi.com/1996-1073/11/5/1085

Abstract:
Liquid lipase-catalyzed esterification of fatty acids with methanol is a promising process for biodiesel production. However, water by-product from this process favors the reverse reaction, thus reducing the reaction yield. To address this, superabsorbent polymer (SAP) was used as a water-removal agent in the esterification in this study. SAP significantly enhanced the conversion yield compared with the reaction without SAP. The lipase-catalyzed esterification in the presence of SAP was then optimized by response surface methodology to maximize the reaction conversion. A maximum conversion of 96.73% was obtained at a temperature of 35.25 °C, methanol to oleic acid molar ratio of 3.44:1, SAP loading of 10.55%, and enzyme loading of 11.98%. Under these conditions, the Eversa Transform lipase could only be reused once. This study suggests that the liquid lipase-catalyzed esterification of fatty acids using SAP as a water-removal agent is an efficient process for producing biodiesel.
50

Rishabh Upadhyay, Soeren Steudel, Mai-Phi Hung, Akhilesh Kumar Mandal, Francky Catthoor and Manoj Nag, “Self-Aligned Amorphous Indium-Tin-Zinc-Oxide Thin Film Transistors on Polyimide Foil”, ECS Journal of Solid State Science and Technology, 2018, 7, 4, 185-191 (ISI: IF 1.808)
http://jss.ecsdl.org/content/7/4/P185.abstract

Abstract:
In this work, we report on high-performance coplanar self-aligned (SA) amorphous-Indium-Tin-Zinc-Oxide (a-ITZO) thin-film transistors (TFTs) on flexible polyimide substrate. The a-ITZO films are first optimized with respect to the oxygen ratio, thickness and final anneal conditions with common-gate TFTs structure on Si/SiO2substrate. Optimized TFTs show mobility (μlin) between 20.0–25.0 cm2/(V.s). Material characterization revealed some degree of order compared to a truly amorphous film like a-IGZO but no grain boundaries or crystalline domains were observed. The a-ITZO films were integrated in coplanar SA TFT architecture on polyimide using hydrogen rich plasma (SiH4 based chemistry) as dopant for the source/drain (S/D) regions resulting in field-effect mobility (μFE) of 27.0 cm2/ (V.s), sub-threshold slope (SS−1) of 0.40 V/decade and ION /IOFF ratio of >108. The threshold voltage shifts of the TFTs under both positive and negative gate bias stress of 1MV/cm for 104 seconds were less than 1.5 V. We have also investigated the applicability of the SA a-ITZO TFTs in logic circuitry such as 19-stage ring-oscillators (ROs).
51

Mai Phi Hung, Christopher Chare, Manoj Nag, A. de Jamblinne de Meux, Jan Genoe and Soere Steudel, “Effect of High Oxygen Partial Pressure on Carrier Transport Mechanism in a-InGaZnO TFTs”, IEEE Transactions on Electron Devices, 2018, 65, 7, 2833-2837 (ISI: IF 2.605)
https://ieeexplore.ieee.org/document/8373744

Abstract:
In this paper, the influence of oxygen partial pressure (P O2 ) during physical vapor deposition on the field-effect mobility of amorphous InGaZnO (IGZO) thin-film transistor (TFT) is investigated in a wide range of P O2 . The field-effect mobility reduced from 12 to 0.1 cm 2 /V · s when the P O2 is increased from 0.3 to 1.5 mTorr. Temperature-dependent TFT measurements suggest that carrier transport in the TFTs deposited at the P O2 of 0.3 mTorr can be described according to the multiple trap and release/percolation model. However, the carrier transport in the TFTs deposited at the P O2 of 1.5 mTorr conforms to Mott's variable-range hopping model. The elastic recoil detection analysis indicates that hydrogen concentration in the IGZO film increased with P O2 . The X-ray photoelectron spectroscopy results indicate that In vacancy (V In ) defects formed in the IGZO film deposited at high P O2 . Scattering induced by hydrogen and pinning of Fermi level below the conduction band edge by V In are plausible reasons for the low field-effect mobility in TFTs deposited at high P O2 .
52

Mai Phi Hung, Jan Genoe, P. Heremans, and Soere Steudel, “Off-current reduction in p-type SnO thin film transistors”, Applied Physics Letters, 2018, 112, 26, 263502 (ISI: IF 3.495)
https://aip.scitation.org/doi/10.1063/1.5037306

Link: https://www.sciencedirect.com/science/article/pii/S0142961218306392

Abstract:
SnO is one of the few candidates for p-type oxide thin film transistors (TFTs) because it retains a reasonable high hole mobility in a nanocrystalline film. However, the high off-current of SnO TFT limits its usefulness. In this work, SnO TFTs were fabricated using thermal evaporation under ultra-high vacuum. In order to decrease the off-current in p-type SnO thin film transistors (TFTs), we used yttrium to reduce n-type minority charges in the channel. The on/off ratio of the TFT increases from 102 to 5 × 104 and the mobility of the TFT in the saturated regime reduces from 1.6 to 1.4 cm2/V s when the SnO channel is doped with 1 wt. % of Y. Grazing incidence X-ray diffraction and X-ray photoelectron spectroscopy reveal that the reduction of SnO2 in the Y-doped SnO TFT channel is the main reason for the improvement in the TFT characteristics.

Bài báo của năm 2017-2018

No Authors
1 Bharath Kumar Velmurugan, Ruey‐Lin Chang, Shibu Marthandam Asokan, Chih‐Fen Chang, Cecilia‐Hsuan Day, Yueh‐Min Lin  Yuan‐Chuan Lin, Wei‐Wen Kuo, Chih‐Yang Huang, “A minireview of E4BP4/NFIL3 in heart failure”, Journal of Cellular Physiology, (2018), doi: 10.1002/jcp.26790
https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.26790
2 Ngoc Tuan Nguyen, “Acinetobacter soli SP2 capable of high-efficiency degradation of food emulsifier polysorbate 80”, Current Microbiology, 75, 7, (2018), 896-900
https://link.springer.com/article/10.1007%2Fs00284-018-1463-7
 
3 Luong Thu-Hien, Nguyen Thanh-Truc, Vo Van Toi, Huynh Chan Khon, Bui Chi Bao, Vo Van Thanh Niem, Mai Ngoc Tuan Anh, Nguyen Dai Hai, Pham Dinh Chuong, Nguyen Thi Hiep, “Evaluation of the morphology and biocompatibility of natural silk fibers/agar blend scaffolds for tissue regeneration”, Hindawi International Journal of Polymer Science, (2018), Article ID 5049728 (7 pages)
https://www.hindawi.com/journals/ijps/2018/5049728/
 
4 Rathinasamy Baskaran, Bharath KumarVelmurugan, “Role of lncRNAs in the cancer development and progression and their regulation by various phytochemicals”, Biomedicine & Pharmacotherapy, 102, (2018), 242-248
https://www.sciencedirect.com/science/article/abs/pii/S0753332218305882
5 Pi-Yu Chao, Meng-Yuan Huang, Wen-Dar Huang, Kuan-Hung Lin, Shiau-Ying Chen, Chi-Ming Yang, “Study of chlorophyll-related compounds from dietary spinach in human blood”, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46, 2, (2018), 309-316
http://www.notulaebotanicae.ro/index.php/nbha/article/view/10918/8102
 
6 Mong-Huai Su, Te-Hua Hsu, Chun-Neng Wang, Kuan-Hung Lin, Ming-Chang Chiang, Ren-Dong Kang, Khiem Minh Nguyen, Hoang Nguyen, “Genetic diversity of a novel oil crop, camellia brevistyla, revealed by ISSR DNA markers”, Horticultural Science and Technology, 35, 5, (2017), 588-598
http://journal.zipot.com/file/9702UBSeKJd86bCVxC5SMb/*/N0130350507.pdf?_=3&authcred=anVzZXJfY3Jvc3NkYjpKb3VybmFsKTk4
7 Kuan-Hung Lin, Penk-Yeir Low, Pi-Yu Chao, Ming-Chih Shih, Ming-Chang Chiang, Yung-Chang Lai, Swi-Bea Wu, “Antioxidant properties and glucose uptake effect of ethanol extracts from different sweet potato leaves prepared by lyophilization and oven-drying at 40 °C”, Current Nutrition & Food Science, 13, 3, (2017), 227-236
http://www.eurekaselect.com/150341/article
 

Tháng 5/2018

No Authors
1 Hoang Chinh Nguyen, Kuan-Hung Lin, Meng-Yuan Huang, Chi-Ming Yang, Tin-Han Shih, Tung-Chuan Hsiung, Yen-Chang Lin, Fun-Chi Tsao, Antioxidant Activities of the Methanol Extracts of Various Parts of Phalaenopsis Orchids with White, Yellow, and Purple Flowers, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2018, 46(2), 457–465.

Tháng 4/2018

No Authors
1 Hoang Chinh Nguyen, Szu-Pei Wu, Chia-Hung Su, Tzann-Shun Hwang, Biochemical, biophysical, and thermal properties of alkaline phosphatase from thermophile Thermus sp. NTU-237, Biotechnology, Agronomy, Society, Environment. 2017 21(2), 117–126(ISI, IF: 0.433). 
2 Hoang Chinh Nguyen, Kuan-Hung Lin, Tung-Chuan Hsiung, Meng-Yuan Huang, Chi-Ming Yang, Jen-Hsien Weng, Ming-Huang Hsu, Po-Yen Chen and Kai-Chieh Chang, Biochemical and Physiological Characteristics of Photosynthesis in Plants of Two Calathea Species, International Journal of Molecular Sciences, 2018, 19(3), 704(ISI, IF: 3.226).

Tháng 3/2018

No Authors
1 Marthandam Asokan Shibu, M. Ravichandran, M.Shanmugavadivu, Bharath Kumar Velmurugan, Pharmacological benefits of Neferine - a comprehensive review, Life Sciences, 2018, 199, 60–70(ISI, IF: 2.936).

Tháng 2/2018

No Authors
1 Thai Thanh Hoang Thi, Yunki Lee, Phuong Le Thi, Ki Dong Park, Nitric oxide-releasing injectable hydrogels with high antibacterial activity through in situ formation of peroxynitrite, Acta Biomaterialia, 2018, 67, 66–78(ISI, IF: 6.319).
2 Helene Kjær Risinggård, Simon Cooil, Federico Mazzola, Di Hu, Marit Kjærvik, Elise Ramleth Østli, Nilesh Patil, Alexei Preobrajenski, D. Andrew Evans, Dag W. Breiby, Thuat T. Trinh, Justin W. Wells, Degradation of the chemotherapy drug 5-fluorouracil on medical-grade silver surfaces, Applied Surface Science, 2018, 435, 1213–1219(ISI, IF: 3.387).

Tháng 1/2018

No Authors
1 Inga Põldsalu, Kätlin Rohtlaid, Tran Minh Giao Nguyen, Cedric Plesse, Frédéric Vidal, Mahdi Safaei Khorram, Anna-Liisa Peikolainen, Tarmo Tamm, Rudolf Kiefer, Thin ink-jet printed trilayer actuators composed of PEDOT:PSS oninterpenetrating polymer networks, Sensors and Actuators B: Chemical, 2018, 258, 1072–1079 (ISI, IF: 5.401).

Tháng 12/2017

No Authors
1 Roshan Khadka, Nihan Aydemir, Arko Kesküla, Tarmo Tamm, Jadranka Travas-Sejdic, Rudolf Kief, Enhancement of polypyrrole linear actuation with poly(ethylene oxide), Synthetic Metals, 2017, 232, 1–7(ISI, IF: 2.435).
2 Madis Harjo, Arko Kesküla, Kaur Leemets, Mahdi Safaei Khorram, Rando Saar,  Martin Järvekülg, Tarmo Tamm, Rudolf Kiefer, Polypyrrole coatings on gelatin fiber scaffolds: Material and electrochemical characterizations in organic and aqueous electrolyte, Synthetic Metals, 2017, 232, 25–30  (ISI, IF: 2.435).
3 Tuan Tran Trong, Dieu-Hien Truong, Dieu-Thai Tran, Huyen-Trang Nguyen Thi, Giap Do Dang, Ho Nguyen Huu, Biomass accumulation of Panax vietnamensis in cell suspension cultures varies with addition of plant growth regulators and organic additives, Asian Pacific Journal of Tropical Medicine,2017,10(9), 907–915  (Scopus).

Tháng 10/2017

No Authors
1 T. M. Ha Vuong, Didier Villemin, Hung-Huy Nguyen, Tin Thanh Le, Tung T. Dang, Hien Nguyen, Programmed Site-Selective Palladium-Catalyzed Arylation of Thieno[3,2-b]thiophene, Chemistry An Asian Journal, 2017, 12(21), 2819–2826 (ISI, IF: 4.083).
2 Le Quoc Bao, Phuong Ho, Ramesh Kumar Chitumalla, Joonkyung Jang, Suresh Thogiti, Jae Hong Kim, Single and double branched organic dyes based on carbazole and red-absorbing cationic indolium for p-type dye-sensitized solar cells: A combined experimental and theoretical investigation, Dyes and Pigments,2018, 149, 25–36 (ISI, IF: 3.473).
3 Thanh M. Nguyen, Senaratne L. Ranamukhaarachchi and Phuc D. A. Nguyen, Pesticide use and health hazards among small-scale commercial vegetable growers in the central highland region of Vietnam, Research on Crops, 2017, 18 (3), 497–507 (Scopus). 

Tháng 9/2017

No Authors
1 Thien-KhanhTran, Kuo-FengChiu, Chiu-YueLin, Hoang-JyhLeu, Electrochemical treatment of wastewater: selectivity of the heavy metals removal process, International journal of hydrogen energy, 2017, 42(45), 27741–27748 (ISI, IF: 3.313). 
2 Bharath Kumar Velmurugan, Chien-Hung Lee, Shang-Lun Chiang, Chun-Hung Hua, Mei-Chung Chen, Shu-Hui Lin, Kun-Tu Yeh, Ying-Chin Ko, PP2A deactivation is a common event in oral cancer and reactivation by FTY720 shows promising therapeutic potential, Journal of Cellular Physiology, 2017, 233(2), 1300–1311 (ISI, IF: 3.839).
3 Meng-Yuan Huang, Kuan-Hung Lin, Chien-Chun Lu, Li-Ru Chen, Tung-Chuan Hsiung, Wen-Ting Chang, the intensity of blue light-emitting diodes influences the antioxidant properties and sugar content of oyster mushrooms (Lentinus sajor-caju), Scientia Horticulturae, 2017, 218, 8–13 (ISI, IF: 1.624).
4 Yu-Duan Liao, Kuan-Hung Lin, Chiu-Chen Chen, Chih Ming Chiang, Oryza sativa protein phosphatase 1a (OsPP1a) involved in salt stress tolerance in transgenic rice, Molecular Breeding, 2016, 36, 22 (ISI, IF: 2.465).
5 Chih-Ming Chiang, Chiu-Chen Chen, Shi-Peng Chen, Kuan-Hung Lin, Li-Ru Chen, Yu-Huei Su, His-Cheng Yen, Overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic Arabidopsis, Journal of Plant Research, 2017, 130(2), 373–386 (ISI, IF: 1.899).

 

 

Bài báo của năm 2016-2017
No Authors
ISI
1 Thanh-Dao Tran, Thi-Cam-Vi Nguyen, Ngoc-Son Nguyen, Dai-Minh Nguyen, Thi-Thu-Ha Nguyen, Minh-Tri Le, Khac-Minh Thai, Synthesis of Novel Chalcones as Acetylcholinesterase Inhibitors, Appl. Sci. 2016, 6(7), 198 (Impact Factor: 1.726).
2 Song Thi Le, Chisa Yasuoka, Haruyasu Asahara and Nagatoshi Nishiwaki, Dual Behavior of Iodine Species in Condensation of Anilines and Vinyl Ethers Affording 2-Methylquinolines. Molecules, 2016, 21, 827 (Impact Factor: 2.465). 
3 Song Thi Le,  Toshiki Fujimoto, Haruyasu Asahara  and   Nagatoshi Nishiwaki,    Synthesis of 6-substituted 2-phenacylpyridines from 2-(phenylethynyl)pyridine via isoxazolo[2,3-a]pyridinium salt, Org. Biomol. Chem., 2016, 14, 10674 – 10682 (Impact Factor: 2.465).
4 Cédric Tarayre, Huu-Thanh Nguyen, Alison Brognaux, Anissa Delepierre, Lies De Clercq, Raphaëlle Charlier, Evi Michels, Erik Meers and Frank Delvigne, Characterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review, Sensors 2016, 16, 797 (Impact Factor: 2.033).
5 Lo Nhat Truong, Marc Dussauze, Mathieu Allix, Sébastien Chenu, Thierry Cardinal, Evelyne Fargin, Luís F. Santos, Second harmonic generation in germanotellurite bulk glassceramics, J. Am. Ceram. Soc. 2017, 1–12 (Impact Factor: 2.787). 
6 Zane Zondaka, Arko Kesküla, Tarmo Tamm, Rudolf Kiefer, Polypyrrole linear actuation tuned by phosphotungstic acid, Sensors and Actuators B: Chemical, 2017, 247, 742-748 (Impact Factor: 4.758).
7 Rudolf Kiefer,  Arko Kesküla, Jose G. Martinez, Gholamreza Anbarjafari, Janno Torop, Toribio F. Otero, Interpenetrated triple polymeric layer as electrochemomechanical actuator: Solvent influence and diffusion coefficient of counterions, Electrochimica Acta, 230, 2017, 461 – 469 (Impact Factor: 1.58).
8 Saber Imani, Chunli Wei, Jingliang Cheng, Md. Asaduzzaman Khan, Shangyi Fu, Luquan Yang, Mousumi Tania, Xianqin Zhang, Xiuli Xiao, Xianning Zhang, Junjiang Fu, MicroRNA-34a targets epithelial to mesenchymal transition-inducing transcription factors (EMT-TFs) and inhibits breast cancer cell migration and invasion, Oncotarget, 2017, 8 (13), 21362 – 21379 (Impact Factor: 5.008).
9 Md. Asaduzzaman Khan, Mousumi Tania, Shangyi Fu, Junjiang Fu, Thymoquinone, as an anticancer molecule: from basic research to clinical investigation, Oncotarget, 2017, doi: 10.18632/oncotarget.17206 (Impact Factor: 5.0). 
10 Jingliang Cheng, Shangyi Fu, ChunliWei, Mousumi Tania, Md. Asaduzzaman Khan, Saber Imania, Baixu Zhou, Hanchun Chen, Xiuli Xiao, JingboWug and Junjiang Fu, Evaluation of PIK3CA mutations as a biomarker in Chinese breast carcinomas from Western China, Cancer Biomark., 2017;19(1):85-92 (Impact Factor: 1.74). 
11 Khon C. Huynh, Thi-Hiep Nguyen, Dinh Chuong Pham, Huong T.T. Nguyen, Toi Van Vo, Marianna Gyenes, Volker R. Stoldt, Integrin αIIbβ3-Dependent ERK Signaling Is Regulated by Src and Rho Kinases in Both Leu33 and Pro33 Polymorphic Isoforms, Acta Haematol 2017, 137, 44–50 (Impact Factor: 1.053).
12 Phung Ngan Le, Dinh Chuong Pham, Dai Hai Nguyen, Ngoc Quyen Tran, Vladimir Dimitrov, Petko Ivanov, Cuong Nguyen Xuan, Hoai Nam Nguyen and Cuu Khoa Nguyen, Poly (N-isopropylacrylamide)-functionalized dendrimer as a thermosensitive nanoplatform for delivering malloapelta B against HepG2 cancer cell proliferation, Advances in Natural Sciences: Nanoscience and Nanotechnology, 2017, 8(2), 025014  (Impact Factor: 1.58).
13 Hoang Chinh Nguyen, Thi Van Anh TRAN, Quoc Linh NGUYEN, Nhu Nhut NGUYEN, Minh Khiem NGUYEN, Ngoc Thanh Tam NGUYEN, Chia-Hung SU, Kuan-Hung LIN, Newly Isolated Paecilomyces lilacinus and Paecilomyces javanicus as Novel Biocontrol Agents for Plutella xylostella and Spodoptera litura, Not Bot Horti Agrobo, 2017, 45(1), 280 – 286 (Impact Factor: 0.48).
14 Hoang Chinh Nguyen, Shih-Hsiang Liang, Thien Thanh Doan, Chia-Hung Su, Pei-Cheng Yang, Lipase-catalyzed synthesis of biodiesel from black soldier fly (Hermetica illucens): Optimization by using response surface methodology, Energy Conversion and Management, 2017, 145, 335-342 (Impact Factor: 5.589). 
15 Thien-KhanhTran, Kuo-FengChiu, Chiu-YueLin, Hoang-JyhLeu, Electrochemical treatment of wastewater: selectivity of the heavy metals removal process, International journal of hydrogen energy, 2017, doi.org/10.1016/j.ijhydene.2017.05.156 (Impact Factor: 3.313).
16 Bharath Kumar Velmurugan, Chien-Hung Lee, Shang-Lun Chiang, Chun-Hung Hua, Mei-Chung Chen, Shu-Hui Lin, Kun-Tu Yeh, Ying-Chin Ko, PP2A deactivation is a common event in oral cancer and reactivation by FTY720 shows promising therapeutic potential, J Cell Physiol.2017, doi: 10.1002/jcp.26001 (Impact Factor: 3.839).
17 Pai-An Hwang, Ming-De Yan, Ko-Liang Kuo, Nam Nhut Phan, Yen-Chang Lin, A mechanism of low molecular weight fucoidans degraded by enzymatic and acidic hydrolysis for the prevention of UVB damage, Journal of Applied Phycology, 29 (1), 521-529 (Impact Factor: 2.616).
18 Nam Nhut Phan, Tung Thanh Huynh, Yen-Chang Lin, Hyperpolarization-Activated Cyclic Nucleotide-Gated Gene Signatures and Poor Clinical Outcome of Cancer Patient, Translational Cancer Research; accepted manuscript, 2017 (Impact Factor: 1.167).
19 Do Thi Duyen, Nam Nhut Phan, Chih-Yang Wang, Zhengda Sun, Yen-Chang Lin, Novel regulations of MEF2-A, MEF2-D, and CACNA1S in the functional incompetence of adipose-derived mesenchymal stem cells by induced indoxyl sulfate in chronic kidney disease, Cytotechnology, 68 (6), 2589-2604(Impact Factor: 1.857).
20 Thuy TD, Phan NN, Wang CY, Yu HG, Wang SY, Huang PL, Do YY, Lin YC, Novel therapeutic effects of sesamin on diabetes-induced cardiac dysfunction, Mol Med Rep. 2017, 15(5), 2949-2956 (Impact Factor: 1.60).
21 Pai-An Hwang, Nam Nhut Phan, Wen-Jung Lu, Bui Thi Ngoc Hieu, Yen-Chang Lin, Low-molecular-weight fucoidan and high-stability fucoxanthin from brown seaweed exert prebiotics and anti-inflammatory activities in Caco-2 cells, Food and nutrition research, 2016, 60, 32033(Impact Factor: 1.676).
Scopus
1 Chih‐Yang Wang, Payam Shahi, John Ting Wei Huang, Nam Nhut Phan, Zhengda Sun, Yen‐Chang Lin, Ming‐Derg Lai, Zena Werb, Systematic analysis of the achaete‐scute complex‐like gene signature in clinical cancer patients, Molecular And Clinical Oncology, 2017, 6(1), 7–18. 
2 Sharanjit Singh, Nguyen Thi Anh Nga, Thong L.M. Pham, Tan Ji Siang, Pham T. T. Phuong, Maksudur R. Khan, Dai-Viet N. Vo, Metgas Production from Bi-reforming of Methane over Lamodified Santa Barbara Amorphous-15 Supported Nickel Catalyst, Chemical Engineering Transactions,  56, 1573-1578

Danh sách các bài ISI (của năm 2015 - 2016)

No Authors
1 Nguyen, H.T., Truong, D.H., Kouhounde, S., Ly, S., Razafindralambo, H., Delvigne, F. (2016) Biochemical Engineering Approaches for Increasing Viability and Functionality of Probiotic Bacteria. Int J Mol Sci, 17(6). Doi:10.3390/ijms17060867 (Impact Factor: 3.257,H index: 64)
2 Do, T.-H., Nguyen, D.M., Truong, V.D., Do, T.H., Le, M.T., Pham, T.Q., Thai, K.M., Tran, T.D. (2016) Synthesis and Selective Cytotoxic Activities on Rhabdomyosarcoma and Noncancerous Cells of Some Heterocyclic Chalcones. Molecules, 21(3). Doi: 10.3390/molecules21030329 (Impact Factor: 2.465, H index: 81)
3 Nguyen, M.-H., Nguyen, T.-H.-N., Hwang, I.-C., Bui, C.-B., Park, H.-J. (2016) Effects of the physical state of nanocarriers on their penetration into the root and upward transportation to the stem of soybean plants using confocal laser scanning microscopy. Crop Protection, 87, 25-30 (Impact Factor: 1.493, H index: 70).
4 Nguyen, M.-H., Tran, T.T., Hadinoto, K. (2016) Controlling the burst release of amorphous drug-polysaccharide nanoparticle complex via crosslinking of the polysaccharide chains. Eur J Pharm Biopharm, 104, 156-163. Doi.org/10.1016/j.ejpb.2016.05.006 (Impact Factor: 4.245, H index: 114).
5 Yung C. Lai, Shu Y. Wang, Huan Y. Gao, Khiem M. Nguyen, Chinh H. Nguyen, Ming C. Shih, Kuan H. Lin (2016) Physicochemical properties of starches and expression and activity of starch biosynthesis-related genes in sweet potatoes. Food Chem, 11,199:556-64. Doi:10.1016/j.foodchem.2015.12.053. (Impact Factor: 3.391, H index: 169 )
6 C.W. Wu, M.C. Lee, Y.L. Peng, T.Y. Chou, K.H.Lin, and Y.S. Chang. (2016) Chlorophyll fluorescence upper-to-lower-leaf ratio for determination of irrigation time for Pentas lanceolate. Photosynthetica, 54 (2): 193-200. DOI: 10.1007/s11099-015-0175-5(Impact Factor: 1.409, H index: 47 )
7 Ngoc Lan Mai, Chan Kyung Kim, Byungho Park, Heon-Jin Park, Sang Huyn Lee, Yoon-Mo Koo. (2016) Prediction of cellulose dissolution in ionic liquids using molecular descriptors based QSAR model. Journal of Molecular Liquids 215, 541-548. Doi:10.1016/j.molliq.2016.01.040 (Impact Factor: 2.515, H index: 52)
8 Ngoc Lan Mai, and Yoon-Mo Koo (2015) Computer-aided design of ionic liquids for high cellulose dissolution. ACS Sustainable Chemistry & Engineering 4 (2), 541-547. DOI: 10.1021/acssuschemeng.5b00958 (Impact Factor: 5.267, H index: 24)
9 Sang-Woo Bae, Doyoung E om, Ngoc Lan Mai and Yoon-Moo Koo. (2016) Refolding of horseradish per oxidase is enhanced in presence of metal cofactors and ionic liquids. Biotechnology Journal 11(4):464-472. (Impact Factor: 3.49, H index: 51)
10 Qing Xie, Kang-Ning Shen, Xiuying Hao, Phan Nhut Nam, Bui Thi Ngoc Hieu, Ching-Hung Chen, Changqing Zhu, Yen-Chang Lin & Chung-Der Hsiao. (2015) The complete chloroplast genome of Tianshan Snow Lotus (Saussurea involucrata), a famous traditional Chinese medicinal plant of the family Asteraceae. Mitochondrial DNA Doi: 10.3109/19401736.2015.1118086 (Impact Factor: 1.209, H index: 25)
11 Nam Nhut Phan, Chih-Yang Wang, Chien-Fu Chen, Zhengda Sun, Ming-Derg Lai, Yen-Chang Lin. (2016) Voltage-gated calcium channels: novel targets for cancer therapy, Oncology Letters (minor revision, consider for publication) (Impact Factor: 1.554, H index:20)
12 Truong, D.H., Bauwens, J., Delaplace, P., Mazzucchelli, G., Lognay, G., Francis, F. (2015) Proteomic analysis of Arabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer). Plant Biology, 17(6), 1210-1217. DOI: 10.1111/plb.12363(Impact Factor: 2.216, H index: 63)
13 Nguyen, H.T., Razafindralambo, H., Richel, A., Jacquet, N., Evrard, P., Antoine, P., Thonart, P., Delvigne, F. (2015) Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria. Journal of Industrial Microbiology & Biotechnology, 42(9), 1225-1231. Doi:10.1007/s10295-015-1650-5 (Impact Factor: 2.732,H index: 80).
14 Truong DH (2015). Variation in volatile profiles of Arabidopsis thaliana Col-0 Heynh plants due to herbivorous infestation: piercing-sucking versus chewing insects. Proceedings of 2015 international Conference on Biologl abd Environmental Sciences (BIOES-15), Phuket,Thailand, October 1-2, 2015.
   

 

Danh sách các bài báo quốc tế (của năm 2014 - 2015)

No Authors
1 Dan, Q.T and Nam Nhut Phan.2015. Optimization of supercritical C02 extraction of oleoresin from black pepper ( Piper nigrum L.) and antioxidant capacity of the oleoresin. International Food research journal, p1489-1493.
2 Chih-Yang Wang, Ming-Derg Lai, Nam Nhut Phan, Zhengda Sun, Yen – Chang Lin. 2015. Meta-analysis of public microarray datasets reveals voltage-gated calcium gene signatures in clinical cancer patients. Plos One, p32
3 Pai-An Hwang, Yu-Lan Hung, Nam Nhut Phan, Hieu Thi Ngoc Bui, Po-Ming Chang, Luan-Lun Li, Yen-Chang Lin. 2015. Cytotechnology, p11

Danh sách các bài báo trước 2014

No Authors
1 Duong Tan Nhut, Tran Ngoc Thuy Tien, Mai Thi Ngoc Huong, Nguyen Thi Thanh Hien, Phan Xuan Huyen, Bui Van Le and Do Nang Vinh. 2004. Some results in study on artificial seeds of Lilium spp. Vietnamese Academy of Science and Technology, Journal of Biotechnology. Vol. 2 (3): 359 – 370.
2 Nguyen Thi Thanh Hien, Dinh Van Khiem, Nguyen Hong Vu, Nguyen Trinh Don and Duong Tan Nhut. 2004. Primary study on the induction and growth of callus of Taxus wallichiana Zucc., a valuable medicinal plant in Lam Dong Province. Journal of Agricultural Sciences and Technology, Nong Lam University. No 4: 79 – 85.
3 Nguyen Thi Thanh Hien, Dinh Van Khiem, Nguyen Hong Vu, Nguyen Trinh Don and Duong Tan Nhut. 2005. Primary study on the induction and growth of callus of Taxus wallichiana Zucc., a valuable medicinal plant in Lam Dong Province. Proceedings of Vietnam-Korea International Symposium on Biotechnology and Bio-system engineering. pp, 191 - 197.
4 Duong Tan Nhut, Tran Ngoc Thuy Tien, Mai Thi Ngoc Huong, Nguyen Thi Thanh Hien, Phan Xuan Huyen, Vu Quoc Luan and Jaime A. Teixeira da Silva. 2005. Artificial seeds for propagation and preservation of Cymbidium spp. Propagation of Ornamental Plants. Vol. 5 (2): 67 - 73.
5 Suriyan Cha-um, Nguyen Thi Thanh Hien, and Chalermpol Kirdmanee. 2006. Desease-free production of Sugarcane varieties (Saccharum officinarum L.) using in vitro meristem culture. Biotechnology Vol. 5 (4): 433 – 448.
6 Duong Tan Nhut, Nguyen Thi Thanh Hien, Nguyen Trinh Don and Dinh Van Khiem. 2007. In vitro shoot development of Taxus wallichiana Zucc., a valuable medicinal plant. In: Mohan Jain S. and Häggman H. (eds), Protocols for Micropropagation of Woods Trees and Fruits, Springer, The Netherlands. pp, 107 – 116.
7 Duong Tan Nhut, Nguyen Trinh Don, Nguyen Thi Thanh Hien, Dinh Van Khiem, Phan Xuan Huyen. 2007. Cell culture and callus recovery from suspension of Hymalayan Yew (Taxus wallichiana Zucc.). Vietnamese Academy of Science and Technology, Journal of Biotechnology Vol. 5 (2): 243 – 253.
8 Ngoc Nhiem Dao, Minh Dai Luu, Quang Khuyen Nguyen and Byung Sun Kim, UV absorption by cerium oxide nanoparticles/epoxy composite thin films, Advances in Natural Sciences: Nanoscience and Nanotechnology, 2 (2011), 045013.
9 Do Tuong Ha, Tran Thanh Dao. Synthesis of some flavones and thioflavones as potential anti-bacterial agents. Science and technology development Journal. The twelfth year. Volume 12 – No 03/2009, p-33-39
10 Tran Phi Hoang Yen, Nguyen Bao Uyen, Vo Phung Nguyen, Đo Tuong Ha, Tran Thanh Dao*. Validation of in-vivo anti-oxidative activity of some flavone semi-synthesized from rutin. Ho Chi Minh City Medical Research, Vol.13, Supp 1, p-157 (01-2009)
11 Tran Le Anh Thuy, Nguyen Thi Thao Nhu, Do Tuong Ha, Vo Thi Anh Thu, Tran Thanh Dao. Synthesis and evaluation of anti-microbial activity of some heterocyclic chalcones. Y hoc Tp.HCM Vol.14 – Supplement of No 1 – 2010 : p.110-115
12 Nguyen Thi Thu Giang, Do Tuong Ha, Khac Minh Thai, Vo Phung Nguyen, Tran Thanh Dao. Synthesis and evalution of anti-inflammatory activity of some Polyoxychalcone. Y hoc Tp.HCM Vol.14 – Supplement of No 1 – 2010 : p-93-99
13 T. Ha Do; T. Thao-Nhu Nguyen; T. Anh-Thu Vo; Thanh-Dao Tran. Synthesis and antibacterial activity against Staphylococus aureus of some heterocyclic chalcone derivatives. Journal of Chemistry, Vol.48 (4B), p. 13 - 18, 11/2010
14 Tran Thi Kim Thoa, Do Tuong Ha, Nguyen Thi Cam Vi, Tran Cat Dong, Tran Thanh Dao. Antimicrobial action of some flavonoids, separate and in combination on the methicillin-resistant Staphycolocus aureus. Pharmaceutical Journal, No 417, Vol.51, p.24-28 (01-2011)
15 Thanh-Dao Tran, Tuong-Ha Do, Ngoc-Chau Tran, Trieu-Du Ngo, Thi-Ngoc-Phuong Huynh, Cat-Dong Tran, and Khac-Minh Thai. Synthesis and anti Methicillin Resistant Staphylococcus aureus activity of substituted chalcones alone and in combination with non-beta-lactam antibiotics. Bioorganic & Medicinal Chemistry Letters, 2012, Vol. 22, in press doi: 10.1016/j.bmcl.2012.05.112
16 Thanh-Dao Tran, Thi-Thao-Nhu Nguyen, Tuong-Ha Do, Thi- Ngoc-Phuong Huynh, Cat-Dong Tran and Khac-Minh Thai. Synthesis and Antibacterial Activity of Some Heterocyclic Chalcone Analogues Alone and in Combination with Antibiotics. Molecules 2012, 17(6), 6684-6696
17 Vo Thanh Phong, Nguyen Thi Thu Ha, Do Tuong Ha, Truong Ngoc Tuyen, Tran Thanh Dao. Synthesis of Nicorandil. Pharmaceutical Journal Vol.18 – Supplement of No 2 – 2014: 391 – 394.
18 Trinh Thi Lan Anh, Ho Thanh Tam, Le Kim Cuong, Nguyen Ba Nam, Nguyen Thi Thanh Hien, Vo Thi Bach Mai, Duong Tan Nhut. Optimization Palaenopsis amabilis from protoplast in breeding. Biotechnology magazine 11 (4): 705 -716, 2013.
19 Nguyen Thi Thu Ha, Truong Ngoc Tuyen, Le Minh Tri, Tran Boi Chau, Tran Thanh Dao. Optimization of parameter for procedure of synthesis of Felodipine. Pharmaceutical Journal Vol 18 – Supplement of No 2-2014: 381-385
20 Truong DH, Bauwens J, Delaplace P, Mazzucchelli G, Lognay G, Francis F. (2015). Proteomic analysis ofArabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer). Plant Biology, 17: 1210-1217.
21 Truong DH (2015). Variation in volatile profiles of Arabidopsis thaliana Col-0 Heynh plants due to herbivorous infestation: piercing-sucking versus chewing insects. Proceedings of 2015 international Conference on Biologl abd Environmental Sciences (BIOES-15), Phuket,Thailand, October 1-2, 2015.
22 Truong DH, Bauwens J, Delaplace P, Mazzucchelli G, Lognay G, Francis F. (2015). Proteomic analysis of Arabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer). Plant Biology, 17: 1210-1217.
23 Nguyen, H-T, Razafindralambo, H., Richel, A., Jacquet, N., Evrard, P., Antoine, P., Thonart, P. and Delvigne, F., 2015. Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria. Journal of Industrial Microbiology & Biotechnology: 1-7. DOI: 10.1007/s10295-015-1650-5
24 Mai, N.L. and Y.-M. Koo, Computer-aided design of ionic liquids for high cellulose dissolution. ACS Sustainable Chemistry & Engineering, 2015.
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