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Publication 2018 – 2019, Faculty of applied sciences, Ton Duc Thang University


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Chao Pi-Yu, Meng-Yuan Huang, Wen-Dar Huang, Kuan-Hung Lin, CHEN Shiau-Ying, YANG Chi-Ming, "Study of Chlorophyll-related Compounds from Dietary Spinach in Human Blood", Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46, 2, (2018), 309-316 (ISI, IF: 0.648).


Human bioavailability data on chlorophyll (Chl) is very limited. The distribution of Chl-related compounds (CRCs) derived from dietary spinach was investigated in human blood. Eight healthy adults, aged from 21 to 61 year-old, consumed 1.2 kg of just-boiled fresh spinach after an 8-h overnight fast. Before and then 3 h after consuming the spinach, blood samples were taken from each participant. Freeze-dried blood samples were prepared, and 80% acetone was added for grinding. Eight peaks were found in the blood using high-performance liquid chromatography (HPLC), and the main CRCs in the samples were pheophytin (Phe) and pheophorbide (Pho) derivates. Compared to a fasted state, markedly higher levels of blood CRCs were detected in all subjects, except that Pho metabolites were not found in two subjects. No significant differences were seen in most of the peaks between males and females; however, relatively higher CRCs levels were observed in females, particular of Pho derivates. In addition, the blood contained significantly higher levels of Phe in the 36~61-year-old group than in the 21~35-year-old group. These results suggest that the conversion of Chls to CRCs is a rapid process, and Chls obtained by ingestion can be absorbed by the human body.


Mong-Huai Su, Te-Hua Hsu, Chun-Neng Wang, Kuan-Hung Lin, Ming-Chang Chiang, Ren-Dong Kang, Khiem M Nguyen, Hoang Nguyen, "Genetic Diversity of a Novel Oil Crop, Camellia brevistyla, Revealed by ISSR DNA Markers", Horticultural Science and Technology, 35, 5, (2017), 588-598 (ISI, IF: 0.365).


Camellia brevistyla plants are used as food oil in Taiwan. To provide a method for identifying germplasm and assessing the genetic diversity of C. brevistyla, 102 individuals were collected from 29 populations in 11 counties throughout the main island of Taiwan. Inter-simple sequence repeat (ISSR) DNA markers were analyzed and a total of 176 bands were amplified. Of these, 175 bands were found to be polymorphic in these populations. Genetic similarities among populations ranged between 34.1-95.1%. Cluster analysis revealed seven groups of populations plus one outlier (C. kissii ). Five individuals from two populations in the southern region were grouped into cluster V, and six populations from both regions were grouped into cluster VII. The remaining populations from the northern region were separated into different clusters from V and VII. Results of an analysis of molecular variance indicated that the variation within populations (75%) was predominantly greater than variations among counties (18%) and among regions (7%). Overall, the gene flow (Nm = 0.509) estimated from genetic differentiation (Gst = 0.534) suggested that gene flow among regions was limited. Higher genetic diversity (H = 0.317), Shannon’s index (I = 0.477), and gene flow (Nm = 0.538) of populations were found in the northern region compared to the southern region. The DNA sequences of C. brevistyla amplified by two oil-biosynthesis genes showed 99-100% homology with those of C. oleifera. Camellia brevistyla populations are highly differentiated and can serve as a basis for the food oil industry as a germplasm resource.


Thanh Mai Nguyen, Nga Thi Thanh Le, Jouni Havukainen, David B Hannaway, "Pesticide use in vegetable production: A survey of Vietnamese farmers’ knowledge", Plant Protection Science, 54, 4, (2018), 203-214 (ISI, IF: 1.076)


Concerns about inappropriate storage, application rates, and disposal practices of pesticides prompted this case study of Vietnamese farmers’ knowledge, attitudes, and practices. 128 small-scale vegetable growers in Lam Dong Province were included in field surveys, questionnaires, and interviews. Farmers reported inappropriate mixing of pesticides and disposal methods. Many also reported ill-timed applications posing potential hazards to the human health and environment. Improved training and monitoring of pesticide residues on foodstuffs and in agricultural soils and community water supplies are needed to ensure safe farmer practices. Community-based training and education, jointly funded by local, national, and international agricultural production and food safety groups, would be a cost-effective method of minimising pesticide applications and improving food safety.


Nam Nhut Phan, Chih‑Yang Wang, Chien‑Fu Chen, Zhengda Sun, Ming‑Derg Lai, Yen‑Chang Lin, "Voltage-gated calcium channels: Novel targets for cancer therapy", Oncology letters, 14, 2, (2017), 2059-2074 (ISI, IF: 1.664)


Voltage-gated calcium channels (VGCCs) comprise five subtypes: The L type; R type; N type; P/Q type; and T type, which are encoded by α1 subunit genes. Calcium ion channels also have confirmed roles in cellular functions, including mitogenesis, proliferation, differentiation, apoptosis and metastasis. An association between VGCCs, a reduction in proliferation and an increase in apoptosis in prostate cancer cells has also been reported. Therefore, in the present study, the online clinical database Oncomine was used to identify the alterations in the mRNA expression level of VGCCs in 19 cancer subtypes. Overall, VGCC family genes exhibited under expression in numerous types of cancer, including brain, breast, kidney and lung cancers. Notably, the majority of VGCC family members (CACNA1C, CACNA1D, CACNA1A, CACNA1B, CACNA1E, CACNA1H and CACNA1I) exhibited low expression in brain tumors, with mRNA expression levels in the top 1 9% of downregulated gene rankings. A total of 5 VGCC family members (CACNA1A, CACNA1B, CACNA1E, CACNA1G and CACNA1I) were under expressed in breast cancer, with a gene ranking in the top 1 10% of the low expressed genes compared with normal tissue. In kidney and lung cancers, CACNA1S, CACNA1C, CACNA1D, CACNA1A and CACNA1H exhibited low expression, with gene rankings in the top 1 8% of downregulated genes. In conclusion, the present findings may contribute to the development of new cancer treatment approaches by identifying target genes involved in specific types of cancer.


Baskaran Rathinasamy, Bharath Kumar Velmurugan, "Role of lncRNAs in the cancer development and progression and their regulation by various phytochemicals", Biomedicine & Pharmacotherapy, 102, (2018), 242-248 (ISI, IF: 3.457)



Long non coding RNAs (lncRNAs) are involved in modulating the expression of other non-coding RNAs (ncRNA), such as microRNAs, or target proteins through the epigenetic, transcriptional, or post-transcriptional regulations. Genomic mutations in cancer reside inside regions that do not code for proteins and these regions are often transcribed into long non coding RNAs (lncRNAs). Emerging evidences have revealed an intense involvement of lncRNAs in the cancer development and progression. Recently, emerging evidences have depicted that the phytochemicals interact with lncRNAs to modulate their activities. Such findings are highly important for the identification of therapeutic strategies against diseases that are particularly associated with an aberrant lncRNA signaling. This review aims at deciphering the role of lncRNAs in the cancer development and progression, and their regulation by various phytochemicals.


Hoang Chinh Nguyen, Tran Quynh Mong Nhu, Phung Van Dung, Nguyen Dinh Hieu, Tran Trong Tuan, Phan Xuan Huyen, Dieu-Hien Truong, "Evaluation of changes in the growth and chemical constituents of Anoectochilus formosanus Hayata grown under hydroponic conditions", BioTechnologia, 99, 4, (2018), 375-383 (Scopus)



Anoectochilus formosanus Hayata is an important medicinal plant with various pharmaceutical properties. In this study, A. formosanus plants were hydroponically cultivated in different nutrient solutions to achieve enhanced biomass and secondary metabolites production. Three months-old A. formosanus plants were grown for 8 weeks under controlled environment in plastic pots containing Murashige and Skoog (MS), Nitrophoska Foliar (NF), Hydro Green (HG), or Hydro Bee (HB) media. Among 4 nutrient solutions tested, HB was the most efficient medium for the plant growth with the highest fresh weight (FW, 2.56 g/plant) and dry weight (DW, 0.18 g/plant) values. The results of phytochemical screening showed the presence of alkaloids, flavonoids, terpenoids, glycosides, and steroids in the extracts of A. formosanus cultivated in HB, HG, and MS medium. The level of these compounds was significantly different in plants cultivated in tested media. The highest alkaloids (34.87 μg/g DW) and terpenoids (56.43 μg/g DW) contents were obtained on HG medium, whereas flavonoids were present in highest amounts (90.13 μg QE/g DW) in plants grown in NF medium. On the other hand, HB medium stimulated the production of the highest glycoside (64.33 μg/g DW) and steroids (22.83 μg/g DW) levels. The antioxidant activity of the extracts was also tested using the 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity assay and the results demonstrated strong antioxidant activities of A. formosanus extracts with IC50 of 136.19 to 248.85 μg/ml. Concluding, the hydroponic-cultivation of A. formosanus is a promising way for obtaining highly valuable compounds for pharmaceutical and nutraceutical industries


Roshan Khadka, Zane Zondaka, Arko Kesküla, Mahdi Safaei Khorram, Tran Thien Khanh, Tarmo Tamm, Jadranka Travas‐Sejdic, Rudolf Kiefer, "Influence of solvent on linear polypyrrole–polyethylene oxide actuators", Journal of Applied Polymer Science, 135, 43, (2018), 46831 (ISI, IF: 1.901)



Ionic conductivity of polypyrrole (PPy) doped with dodecylbenzenesulfonate (DBS) was improved by loading of 10 wt % of polyethylene oxide (PEO). The linear actuation properties of PPy–PEO/DBS films were investigated and compared in polar (aqueous) and aprotic (propylene carbonate) solvents keeping the concentration of lithium bis(trifluoromethane)sulfonimide (LiTFSI) electrolyte the same. The results were compared to those of pristine PPy/DBS films. The actuation direction changed from pure cation‐driven in the aqueous electrolyte (LiTFSI(aq)) to anion‐driven in propylene carbonate electrolyte (LiTFSI(PC)). At the same time, the electro‐chemo‐mechanical deformation revealed that the strain of PPy–PEO/DBS increased from 5% in LiTFSI(aq) to 20% in LiTFSI(PC). The effect of solvent on the ionic conductivity was investigated using electrochemical impedance spectroscopy, showing a 27% increase in charge transfer kinetics and an increase in the electronic conductivity, resulting in significant increase in the strain rate, when propylene carbonate electrolyte was used.


Zane Zondaka, Alo Kivilo, Sunjai Nakshatharan, Kadi-Anne Küppar, Urmas Johanson, Tarmo Tamm, Rudolf Kiefer, "Carbide-derived carbon and poly-3, 4-ethylenedioxythiphene composite laminate: linear and bending actuation", Synthetic Metals, 245, (2018), 67-73 (ISI, IF: 2.526)


Carbide-derived carbon is a well-known material for ionic electroactive systems like sensors and actuators. Typically, a gold foil or other current collector is applied on top of either side to enhance the conductivity of such laminates. Here, metal foil is replaced by an electrochemically deposited layer of poly-3,4-ethylenedioxythiophene (PEDOT). While lower in conductivity, PEDOT has the advantages of lowered risk of delamination and being electro-chemo-mechanically active itself. Such laminates were tested in both linear actuation mode in lithium bis(trifluoromethane) sulfonamide propylene carbonate solution (LiTFSI-PC) under isotonic and isometric electro-chemo-mechanical deformation measurements (ECMD), and in bending mode in air using a room-temperature ionic liquid as the electrolyte. The exchanged charge as well as the actuation response of the laminate was dominated by the PEDOT layer, overriding even the actuation direction of the carbon-based layers. The stress of all investigated samples was found in similar range of 30 kPa. The materials were characterized by scanning electron microscopy (SEM), FTIR and energy-dispersive X-Ray spectroscopy (EDX).


Bharath Kumar Velmurugan, Wei-Hsiang Chang, Chia-Min Chung, Chung-Min Yeh, Chien-Hsun Lee, Kun-Tu Yeh, Shu-Hui Lin, "DDR2 overexpression in oral squamous cell carcinoma is associated to lymph node metastasis", Cancer Biomarkers, 22, 4, (2018), 747-753 (ISI, IF: 2.392)



BACKGROUND: Discoidin domain receptors (DDRs), a collagen receptor tyrosine kinase, play a major role in cancer progression. DDR2 has been suggested as a prognostic marker in several cancer types; however, the correlation between DDR2 expression and clinical outcome of oral cancer patients in Taiwan population has not been investigated. MATERIALS AND METHODS: In the present study we sought to determine the clinical significance of Discoidin Domain Receptor Tyrosine Kinase 2 (DDR2) expression in oral squamous cell carcinoma (OSCC) patients. We examined DDR2 expression in OSCC specimens by immunohistochemistry and then we analyzed the association of DDR2 expression with clinicopathological factors in OSCC. RESULTS: We divided 254 OSCC cases into two groups based on DDR2 expression levels and compared with several clinicopathological factors and their overall survival. The group with high DDR2 expression had significantly higher frequencies of lymph node metastasis (P= 0.0094) and AJCC stage (P= 0.0058) compared to the group with low DDR2 expression. Furthermore, the lymph node metastasis oral cancer patients with high DDR2 expression had low survival rate than low DDR2 group (P= 0.0458). CONCLUSIONS: Our data indicate that DDR2 is a potent biomarker that can be used as an effective therapeutic target for treating OSCC patients with lymph node metastasis.


Shang-Lun Chiang, Bharath Kumar Velmurugan, Chia-Min Chung, Shu-Hui Lin, Zhi-Hong Wang, Chun-Hung Hua, Ming-Hsui Tsai, Tzer-Min Kuo, Kun-Tu Yeh, Pei-Ying Chang, "Preventive effect of celecoxib use against cancer progression and occurrence of oral squamous cell carcinoma", Scientific reports, 7, 1, (2017), 6235  (ISI, IF: 4.122)



Overexpression of cyclooxygenase-2 in oral cancer increases lymph node metastasis and is associated with a poor prognosis. The potential of celecoxib (CXB) use is reported in cancer treatment by inhibiting proliferation through apoptosis, but the effects on the epithelial-mesenchymal transition (EMT) and cancer cell mobility remain unclear. We performed a preclinical study and population-based study to evaluate CXB use in the prevention of oral cancer progression and occurrence. The in-vitro findings showed that CXB is involved in the inhibition of EMT and cell mobility through blocking transcription factors (Slug, Snail and ZEB1), cytoplasmic mediators (focal adhesion kinase (FAK), vimentin and β-catenin), cell adhesion molecules (cadherins and integrins), and surface receptors (AMFR and EGFR). The murine xenograft model showed a 65% inhibition in tumour growth after a 5-week treatment of CXB compared to placebo. Xenograft tumours in placebo-treated mice displayed a well-to-moderate/moderate differentiated SCC grade, while those from CXB-treated mice were well differentiated. The expression levels of membrane EGFR, and nuclear FAK, Slug and ZEB1 were decreased in the xenograft tumours of CXB-treated mice. A retrospective cohort study showed that increasing the daily dose and medication time of CXB was associated with oral cancer prevention. The findings provide an alternative prevention strategy for oral cancer development with CXB use.


Su‐Ying Wen, Bharath Kumar Velmurugan, Cecilia Hsuan Day, Chia‐Yao Shen, Li‐Chin Chun, Yi‐Chieh Tsai, Yueh‐Min Lin, Ray‐Jade Chen, Chia‐Hua Kuo, Chih‐Yang Huang, "High density lipoprotein (HDL) reverses palmitic acid induced energy metabolism imbalance by switching CD36 and GLUT4 signaling pathways in cardiomyocyte", Journal of cellular physiology, 232, 11, (2017), 3020-3029 (ISI, IF: 3.923)



In our previous study palmitic acid (PA) induced lipotoxicity and switches energy metabolism from CD36 to GLUT4 in H9c2 cells. Low level of high density lipoprotein (HDL) is an independent risk factor for cardiac hypertrophy. Therefore, we in the present study investigated whether HDL can reverse PA induced lipotoxicity in H9c2 cardiomyoblast cells. In this study, we treated H9c2 cells with PA to create a hyperlipidemia model in vitro and analyzed for CD36 and GLUT4 metabolic pathway proteins. CD36 metabolic pathway proteins (phospho‐AMPK, SIRT1, PGC1α, PPARα, CPT1β, and CD36) were decreased by high PA (150 and 200 μg/μl) concentration. Interestingly, expression of GLUT4 metabolic pathway proteins (p‐PI3K and pAKT) were increased at low concentration (50 μg/μl) and decreased at high PA concentration. Whereas, phospho‐PKCζ, GLUT4 and PDH proteins expression was increased in a dose dependent manner. PA treated H9c2 cells were treated with HDL and analyzed for cell viability. Results showed that HDL treatment induced cell proliferation efficiency in PA treated cells. In addition, HDL reversed the metabolic effects of PA: CD36 translocation was increased and reduced GLUT4 translocation, but HDL treatment significantly increased CD36 metabolic pathway proteins and reduced GLUT4 pathway proteins. Rat neonatal cardiomyocytes showed similar results. In conclusion, HDL reversed palmatic acid‐induced lipotoxicity and energy metabolism imbalance in H9c2 cardiomyoblast cells and in neonatal rat cardiomyocyte cells.


Peiying Pai, Bharath Kumar Velmurugan, Chia-Hua Kuo, Chung-Yi Yen, Tsung-Jung Ho, Yueh-Min Lin, Yu-Feng Chen, Chao-Hung Lai, Cecilia Hsuan Day, Chih-Yang Huang, "17β-Estradiol and/or estrogen receptor alpha blocks isoproterenol-induced calcium accumulation and hypertrophy via GSK3β/PP2A/NFAT3/ANP pathway", Molecular and cellular biochemistry, 434, 1-2, (2017), 181-195 (ISI, IF: 2.561)



The present study was aimed to investigate the protective effects of 17β-estradiol (E2) and estrogen receptor α (ERα) on isoproterenol (ISO)-treated H9c2 cardiomyoblast cells. In the present study, we treated H9c2 cells with ISO, a β-adrenergic receptor agonist, to induce myocardiac hypertrophy. Pre-administration of E2 or ERα (induced by doxycycline) and E2 plus ERα significantly prevented ISO-induced increase of cell size and cytosolic calcium accumulation, accompanied with increased mRNA of atrial natriuretic peptide and brain natriuretic peptide. However, ICI-ERs antagonist, and melatonin, a specific inhibitor for ERα, reversed the cardioprotective effects, suggesting that E2 action was mediated through ERα. Further evidences showed that E2 and ERα increased the protein level of GSK3β and protein phosphatase 2a inhibitor 2 (I2-PP2A), which subsequently enhanced the activation of I2-PP2A by disrupting PP2A activity and maintains normal calcium outflow. Collectively, E2 and ERα inhibited hypertrophy by preventing cytosol calcium accumulation and by inhibiting the association between PP2A with Na+–Ca2+ exchanger via GSK3β and I2-PP2A activation


Rathinasamy Baskaran, Bharath Kumar Velmurugan, "Protein phosphatase 2A as therapeutic targets in various disease models", Life sciences, 210, 1, (2018), 40-46 (ISI, IF: 3.234)



There are a large number of signalling pathways responsible for transmitting information within the cell. Although cellular signalling is thought to be majorly governed by protein kinases ‘cascade effects’; their antagonists protein phosphatases also play a crucial dual role in signal transduction. By dephosphorylating the proteins involved in signalling pathways, phosphatases may lead to their activation and sometimes they may terminate a signal generated by kinases activity. Due to counterbalancing the function of phosphorylation, the protein phosphatases are very important to signal transduction processes and thus the control of phosphatase activity is as significant as kinases, in the regulation of a plethora of cellular processes. In general, the protein phosphatases are comprised of a catalytic subunit with one or more regulatory and/or targeting subunits associated with it. The Protein Phosphatase 2A (PP2A), a member of serine/threonine phosphatases family, is ubiquitously expressed a remarkably conserved enzyme in the cell. Its catalytic activity has been highly regulated and may have enormous therapeutic potential which is still untapped. It has specificities for a number of substrates which witnessed its involvement in various signalling modules of cell cycle regulation, cell morphology and development. Thus it can be an appropriate target for studying different diseases associated with abnormal signal transduction pathways such as neurodegenerative diseases and malignancies. This review will focus on the structure and regulatory pathways of PP2A. The de-regulation of PP2A in some specific pathology such as Cancer, Heart diseases, Neurodegenerative disorders and Diabetes will also be touched upon


Nancy Maurya, Bharath Kumar Velmurugan, "Therapeutic applications of cannabinoids", Chemico-biological interactions, 293, (2018), 77-88 (ISI, IF: 3.296)



The psychoactive property of cannabinoids is well known and there has been a continuous controversy regarding the usage of these compounds for therapeutic purposes all over the world. Their use for medical and research purposes are restricted in various countries. However, their utility as medications should not be overshadowed by its negative physiological activities. This review article is focused on the therapeutic potential and applications of phytocannabinoids and endocannabinoids. We further highlights their mode of action, overall effects on physiology, various in vitro and in vivo studies that have been done so far and the extent to which these compounds can be useful in different disease conditions such as cancer, Alzheimer's disease, multiple sclerosis, pain, inflammation, glaucoma and many others. Thus, this work is an attempt to make the readers understand the positive implications of these compounds and indicates the significant developments of utilizing cannabinoids as therapeutic agents



Tran Thien Khanh, Rong-Jer Lee, Paul A Kilmartin, Md Asaduzzaman Khan, Mahdi Safaei Khorram, Tarmo Tamm, Rudolf Kiefer, "Actuation increase in polypyrrole bilayer by photo-activated dopants", Synthetic Metals, 246, (2018), 57-63 (ISI, IF: 2.526)



A new methodology to increase the polypyrrole (PPy) bilayer actuation displacement is presented, based on photo-activated dopants generating secondary charges. Two dopants, dodecyl benzenesulfonate (DBS) and the photo-active dopant 2-diazo-1-naphthol-5-sulfonic acid (DNSA), were compared in this study. PPy/DBS, PPy/DBS-DNSA and PPy/DNSA bilayers on polyethylene terephthalate were formed and their actuation properties in aqueous electrolyte were investigated applying cyclic voltammetry and square wave potential steps. Exposure to solar irradiation increased PPy/DBS-DNSA and PPy/DNSA bilayer bending displacements by two and three times, respectively, accompanied by increased charge density during the reversible redox cycles. UV–vis and Fourier transform infrared (FTIR) measurements were also performed to follow the photo reaction of the photo-active dopants


M Shanmugavadivu, Bharath Kumar Velmurugan, "Pharmalogical activities of antroquinonol-Mini review", Chemico-biological interactions, 297, 5, (2018), 8-15 (ISI, IF: 3.296)



Antrodia camphorata is an expensive mushroom that grows on the inner cavity of an endangered native tree of Taiwan namely Cinnamomum kanehirai Hayata. It is used as a traditional medicine in Taiwan and has several health benefits including free radical scavenging, anti-inflammatory, antimicrobial, hepatoprotective, neuroprotective, antidiabetic, and free radical-induced DNA damage protecting activities. Antroquinonol is a tetrahydro ubiquinone derivative found predominately in the mycelium of Antrodia camphorata, and is characterized by numerous biological and pharmacological activities. Several studies have revealed potential anticancer effects of antroquinonol in various carcinogenic models. Moreover, a phase II clinical trial is ongoing in the US and Taiwan to treat the lung cancer patients with this active compound. The present review aims at depicting a detailed view of the synthetic procedures of antroquinonol as well as deciphering its potential health benefits with a special emphasis on anticancer properties.


Lin Kuan-Hung, WU Chun-Wei, Yu-Sen Chang, "Applying Dickson Quality Index, Chlorophyll Fluorescence, and Leaf Area Index for Assessing Plant Quality of Pentas lanceolata", Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47, 1, (2018), 169-176 (ISI, IF: 0.648)



Plant quality greatly relates to the seedling vigor (SV), survival and growth of plants after transplantation. The objective of this study was to use the nondestructive measurements of chlorophyll fluorescence (ChlF) and leaf area index (LAI) as SV indices for star cluster (Pentas lanceolata). Plants were grown in potting soil under nature sunlight for 90 d. A total of 13 morphological and physiological parameters were selected for measurements. Among them, root growth potential (RGP) was the best predictor for SV in all tested plants. Plants were separated into 5 RGP groups based on the number of new roots, and remaining parameters were also separated into those same levels. The trends and rates of increase from levels 1 to 5 in Dickson quality index (DQI), LAI, total dry mass, and ChlF were all similar to the RGP index. Although RGP and DQI are frequently used as indices for SV, these measurements are time-consuming and require sample destruction. Consistent and strongly high correlations were observed among DQI, LAI, and ChlF, demonstrating the applicability of these indices for measuring SV in star cluster. The measurements of LAI and ChlF were predicted using multiple variables from validation datasets, and showed novel and useful parameters for examining the SV of star cluster.


Dinh-Chuong Pham, Yu-Chuan Chang, Shian-Ren Lin, Yuh-Ming Fuh, May-Jywan Tsai, Ching-Feng Weng, "FAK and S6K1 inhibitor, Neferine, dually induces autophagy and apoptosis in human neuroblastoma cells", Molecules, 23, 12, (2018), 3110 (ISI, IF: 3.098)



Human neuroblastoma cancer is the most typical extracranial solid tumor. Yet, new remedial treatment therapies are demanded to overcome its sluggish survival rate. Neferine, isolated from the lotus embryos, inhibits the proliferation of various cancer cells. This study aimed to evaluate the anti-cancer activity of neferine in IMR32 human neuroblastoma cells and to expose the concealable molecular mechanisms. IMR32 cells were treated with different concentrations of neferine, followed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess cell viability. In an effort to determine the molecular mechanisms in neferine-incubated IMR32 cells, cell cycle arrest, cell migration, and focal adhesion kinase (FAK), the 70-kDa ribosomal S6 kinase 1 (S6K1), poly (ADP-ribose) polymerase (PARP), caspase-3, Beclin-1, and microtubule-associated protein 1A/1B-light chain 3 (LC3) protein expressions were investigated. Neferine strongly disrupted the neuroblastoma cell growth via induction of G2/M phase arrest. Furthermore, neferine provoked autophagy and apoptosis in IMR32 cells, confirmed by p-FAK, and p-S6K1 reduction, LC3-II accumulation, Beclin-1 overexpression, and cleaved caspase-3/PARP improvement. Finally, neferine markedly retarded cell migration of neuroblastoma cancer cells. As a result, our findings for the first time showed an explicit anti-cancer effect of neferine in IMR32 cells, suggesting that neferine might be a potential candidate against human neuroblastoma cells to improve clinical outcomes with further in vivo investigation.


Zane Zondaka, Madis Harjo, Mahdi Safaei Khorram, Pejman Rasti, Tarmo Tamm, Rudolf Kiefer, "Polypyrrole/carbide-derived carbon composite in organic electrolyte: Characterization as a linear actuator", Reactive and Functional Polymers, 131, (2018), 414-419 (ISI, IF: 2.975)



Polypyrrole (PPy) doped with dodecylbenzenesulphonate (DBS) (PPy/DBS) was polymerized with the addition of phosphotungstic acid (PTA), thus, incorporating multicharged phosphotungstate anions (PT) to give PPy/DBS-PT films. With carbide-derived carbon (CDC) particles included, the obtained films contained CDC-PT, DBS and PT anions forming PPy/DBS-CDC-PT (PPy/CDC). Our goal was to test the applicability of the material for actuation in an organic electrolyte. The material properties of PPy/CDC films, such as conductivity, charging/discharging and actuation as strain and strain rate were significantly changed in comparison to PPy/DBS-PT films. FTIR (Fourier transform infrared) and EDX (energy dispersive X-ray) spectroscopy revealed that CDC-PT is incorporated in the PPy films and the SEM (scanning electron microscopy) images showed a more porous film with CDC particles packed into PPy. Electro-chemo-mechanical deformation studies (ECMD) revealed that PPy/CDC films had anion-dominated actuation resulting in nearly 6 times higher strain, 2 times higher force, higher strain rates, and 7 times higher conductivity than PPy/DBS-PT films, which had mixed ion transport and rather poor strain and stress behavior. Thus, only one of the two materials - PPy/CDC – could have some practical use in this type of electrolyte solutions.


P Muralidhar Reddy, Nancy Maurya, Bharath Kumar Velmurugan, "Medicinal Use of Synthetic Cannabinoids—a Mini Review", Current Pharmacology Reports, 5, 1, (2019), 1-13 (Scopus)



Purpose of Review

This review gives an overview of the medicinal uses of synthetic cannabinoids and other related aspects on the basis of recent as well as earlier studies that the authors considered relevant to the context and scope of the review.

Recent Findings

Synthetic cannabinoids are laboratory synthesized products eliciting effects way more than their natural counterparts. These compounds are more potent in generating intoxicating effects and are also difficult to be detected in conventional screening tests. Their clinical side effects are also more pronounced than natural cannabinoids, and their antidotes are also not known. However, they are also therapeutically found to be very effective in many health conditions, as these act by interacting with almost ubiquitously distributed cannabinoid receptors (CB1 and CB2) in the human body and by other mechanisms also that do not involve these receptors.


All the issues related to their appropriate dosage, mode of action, acute and chronic effects in vivo, interaction with other drugs, their metabolism, etc. need much research to be done so that it will be easier to predict their different aspects in human subjects in more appropriate way. Further, development of strict legislation and regulation is required to be done so that their abuse can be curbed, and toxic effects can be reduced, but medicinal benefits and usage can be enhanced.


VH Giang Phan, Huu Thuy Trang Duong, Thavasyappan Thambi, Thanh Loc Nguyen, Md Hasan Turabee, Yue Yin, Seong Han Kim, Jaeyun Kim, Ji Hoon Jeong, Doo Sung Lee, "Modularly engineered injectable hybrid hydrogels based on protein-polymer network as potent immunologic adjuvant in vivo", Biomaterials, 195, (2019), 100-110 (ISI, IF: 8.806)



Lymphoid organs, which are populated by dendritic cells (DCs), are highly specialized tissues and provide an ideal microenvironment for T-cell priming. However, intramuscular or subcutaneous delivery of vaccine to DCs, a subset of antigen-presenting cells, has failed to stimulate optimal immune response for effective vaccination and need for adjuvants to induce immune response. To address this issue, we developed an in situ-forming injectable hybrid hydrogel that spontaneously assemble into microporous network upon subcutaneous administration, which provide a cellular niche to host immune cells, including DCs. In situ-forming injectable hybrid hydrogelators, composed of protein-polymer conjugates, formed a hydrogel depot at the close proximity to the dermis, resulting in a rapid migration of immune cells to the hydrogel boundary and infiltration to the microporous network. The biocompatibility of the watery microporous network allows recruitment of DCs without a DC enhancement factor, which was significantly higher than that of traditional hydrogel releasing chemoattractants, granulocyte-macrophage colony-stimulating factor. Owing to the sustained degradation of microporous hydrogel network, DNA vaccine release can be sustained, and the recruitment of DCs and their homing to lymph node can be modulated. Furthermore, immunization of a vaccine encoding amyloid-β fusion proteinbearing microporous network induced a robust antigen-specific immune response in vivo and strong recall immune response was exhibited due to immunogenic memory. These hybrid hydrogels can be administered in a minimally invasive manner using hypodermic needle, bypassing the need for cytokine or DC enhancement factor and provide niche to host immune cells. These findings highlight the potential of hybrid hydrogels that may serve as a simple, yet multifunctional, platform for DNA vaccine delivery to modulate immune response.


Nguyen Quang Khuyen, Jose G Martinez, Friedrich Kaasik, Tarmo Tamm, Toribio F Otero, Rudolf Kiefer, "Solvent effects on carbide-derived-carbon trilayer bending actuators", Synthetic Metals, 247, (2019), 170-176 (ISI, IF: 2.526)



Bending actuators were prepared by depositing carbide-derived carbon, a material typical for electric double layer capacitors, on both sides of poly-vinylidenefluoride membranes, forming CDC-trilayers. Their actuation properties were studied using 0.5 M solutions of lithium perchlorate (LiClO4) in different solvents: water, ethylene glycol, acetonitrile, and propylene carbonate. The goal of this work was to study the actuation mechanism, charging-discharging properties in these solvents, as well as to establish the optimal solvent for maximum bending displacement. It was found that while the actuation direction was the same for all solvents, pointing to similar mechanism, the exchanged charge and the displacement differed considerably. Moreover, the highest specific capacitance found in ethylene glycol did not bring along the highest displacement, neither was the highest exchanged charge of propylene carbonate the most efficient option, the acetonitrile was the clear winner. The available electrochemical windows for the reversible charging also differed considerably.


Thai Thanh Hoang Thi, Thi Nhu Quynh Nguyen, Duc Thuan Hoang, Van Cuong Ngo, Dai Hai Nguyen, "Functionalized mesoporous silica nanoparticles and biomedical applications", Materials Science and Engineering: C, 99, (2019), 631-656 (ISI, IF: 5.080)



Since the first report in early 1990s, mesoporous silica nanoparticles (MSNs) have progressively attracted the attention of scientists due to their potential applications in physic, energy storage, imaging, and especially in biomedical engineering. Owning the unique physiochemical properties, such as highly porosity, large surface area and pore volume, functionalizable, tunable pore and particle sizes and biocompatibility, and high loading cavity, MSNs offer efficient encapsulation and then controlled release, and in some cases, intracellular delivery of bioactive molecules for biomedical applications. During the last decade, functionalized MSNs that show respond upon the surrounding stimulus changes, such as temperature, pH, redox, light, ultrasound, magnetic or electric fields, enzyme, redox, ROS, glucose, and ATP, or their combinations, have continuously revolutionized their potential applications in biomedical engineering. Therefore, this review focuses on discussion the recent fabrication of functionalized MSNs and their potential applications in drug delivery, therapeutic treatments, diagnostic imaging, and biocatalyst. In addition, some potential clinical applications and challenges will also be discussed


Hoang Chinh Nguyen, Chia-Hung Su, Yuan-Kun Yu, Dinh Thi My Huong, "Sugarcane bagasse as a novel carbon source for heterotrophic cultivation of oleaginous microalga Schizochytrium sp", Industrial crops and products, 121, (2018), 99-105 (ISI, IF: 3.849)



Oleaginous microalgae are known as promising oil producers, which accumulate high amount of lipids from various carbon substrates. This study first investigated sugarcane bagasse hydrolysate as a cheap carbon source for the production of biomass and lipid by Schizochytrium sp. The sugarcane bagasse was pretreated with alkali followed by phosphoric acid to remove lignin and enhance xylose production, respectively. The enzymatic hydrolysis of the pretreated sugarcane bagasse by cellulase was subsequently optimized. A maximum glucose yield of 95.77% was obtained at an enzyme loading of 0.3 mL/g with a hydrolysis reaction time of 48 h. The sugarcane bagasse hydrolysate containing glucose and xylose was subsequently used as a substrate for cultivating Schizochytrium sp. Results showed that sugarcane bagasse hydrolysate performed better than refined glucose for cell growth and lipid accumulation. The maximum biomass (10.45 g/L) and lipid content (45.15%) were achieved by growing Schizochytrium sp. in a medium containing 40 g/L glucose in sugarcane bagasse hydrolysate for 72 h. Sugarcane bagasse hydrolysate also resulted in higher levels of polyunsaturated fatty acid and docosahexaenoic acid than did refined glucose. This study suggests that sugarcane bagasse hydrolysate is a low-cost and effective carbon source for microalgal biomass and lipid production


Hoang Chinh Nguyen, Shih-Hsiang Liang, Shang-Sian Chen, Chia-Hung Su, Jhih-Huei Lin, Chien-Chung Chien, "Enzymatic production of biodiesel from insect fat using methyl acetate as an acyl acceptor: optimization by using response surface methodology", Energy Conversion and Management, 158, (2018), 168-175 (ISI, IF: 6.377)



Black soldier fly larvae (BSFL) are oleaginous insects that can assimilate organic waste for fat accumulation, and thus serve as an alternative feedstock for biodiesel production. In lipase-catalyzed transesterification, enzymes are deactivated by excess methanol. To address this obstacle, methyl acetate is suggested as an alternative acyl acceptor to methanol. In this study, methyl acetate was first used in the enzymatic production of biodiesel with BSFL as a triglyceride source. The interesterification of BSFL fat with methyl acetate was catalyzed using Novozym 435 as an efficient immobilized lipase. Response surface methodology was used to optimize the reaction and establish a reliable mathematical model for prediction. A maximum biodiesel yield of 96.97% was reached at a reaction time of 12 h, molar ratio of methyl acetate to fat of 14.64: 1, enzyme loading of 17.58%, and temperature of 39.5 °C. Under these optimal reaction conditions, Novozym 435 could be reused for up to 20 cycles without loss in enzyme activity. The properties of BSFL biodiesel were also investigated and all met the European standard EN 14214. This study indicates that the enzymatic interesterification of BSFL fat with methyl acetate is a promising and ecofriendly method for green fuel production.


Long‐Bin Jeng, Bharath Kumar Velmurugan, Hsi‐Hsien Hsu, Su‐Ying Wen, Chia‐Yao Shen, Chih‐Hao Lin, Yueh‐Min Lin, Ray‐Jade Chen, Wei‐Wen Kuo, Chih‐Yang Huang, "Fenofibrate induced PPAR alpha expression was attenuated by oestrogen receptor alpha overexpression in Hep3B cells", Environmental toxicology, 33, 2, (2018), 234-247 (ISI, IF: 2.491)



The physiological regulation of Oestrogen receptor α (ERα) and peroxisome proliferator‐activated receptor alpha (PPARα) in Hepatocellular carcinoma (HCC) remains unknown. The present study we first treat the cells with fenofibrate and further investigated the possible mechanisms of 17β‐estradiol (E2) and/or ERα on regulating PPARα expression. We also found higher PPARα expression in the tumor area than adjacent areas and subsequently compared PPARα expression in four different hepatic cancer cell lines. Hep3B cells were found to express more PPARα than the other cell lines. Using the PPARα agonist fenofibrate, we found that fenofibrate increased Hep3B cell proliferation efficiency by increasing cell cycle proteins, such as cyclin D1 and PCNA, and inhibiting p27 and caspase 3 expressions. Next, we performed transient transfections and immuno‐precipitation studies using the pTRE2/ERα plasmid to evaluate the interaction between ERα and PPARα. ERα interacted directly with PPARα and negatively regulated its function. Moreover, in Tet‐on ERα over‐expressed Hep3B cells, E2 treatment inhibited PPARα, its downstream gene acyl‐CoA oxidase (ACO), cyclin D1 and PCNA expression and further increased p27 and caspase 3 expressions. However, over‐expressed ERα plus 17‐β‐estradiol (10−8 M) reversed the fenofibrate effect and induced apoptosis, which was blocked in ICI/melatonin/fenofibrate‐treated cells. This study illustrates that PPARα expression and function were negatively regulated by ERα expression in Hep3B cells.


Bharath Kumar Velmurugan, Lohanathan Bharathi Priya, Paramasivan Poornima, Li‐Jen Lee, Rathinasamy Baskaran, "Biomaterial aided differentiation and maturation of induced pluripotent stem cells", Journal of cellular physiology, 234, 6, (2019), 8443-8454 (ISI, IF: 3.923)


Engineering/reprogramming differentiated adult somatic cells to gain the ability to differentiate into any type of cell lineage are called as induced pluripotent stem cells (iPSCs). Offering unlimited self‐renewal and differentiation potential, these iPSC are aspired to meet the growing demands in the field of regenerative medicine, tissue engineering, disease modeling, nanotechnology, and drug discovery. Biomaterial fabrication with the rapid evolution of technology increased their versatility and utility in regenerative medicine and tissue engineering, revolutionizing the stem cell biology research with the property to guide the process of proliferation, differentiation, and morphogenesis. Combining traditional culture platforms of iPSC with biomaterials aids to overcome the limitations associated with derivation, proliferation, and maturation, thereby could improve the clinical translation of iPSC. The present review discusses in brief about the reprogramming techniques for the derivation iPSC and details on several biomaterial guided differentiation of iPSC to different cell types with specific relevance to tissue engineering/regenerative medicine

Long‐Bin Jeng, Bharath Kumar Velmurugan, Ming‐Cheng Chen, Hsi‐Hsien Hsu, Tsung‐Jung Ho, Cecilia‐Hsuan Day, Yueh‐Min Lin, V Vijaya Padma, Chuan‐Chou Tu, Chih‐Yang Huang, "Fisetin mediated apoptotic cell death in parental and Oxaliplatin/irinotecan resistant colorectal cancer cells in vitro and in vivo", Journal of cellular physiology, 233, 9, (2018), 7134-7142 (ISI, IF: 3.923)



Irinotecan (CPT11) and Oxaliplatin have been used in combination with fluorouracil and leucovorin for treating colorectal cancer. However, the efficacy of these drugs is reduced due to various side effects and drug resistance. Fisetin, a hydroxyflavone possess anti‐proliferative, anti‐cancer, anti‐inflammatory, and antioxidant activity against various types of cancers. Apart from that, fisetin has been shown to induce cytotoxic effects when combined with other known chemotherapeutic drugs. In this study, we aimed to investigate whether Fisetin was capable of sensitizing both Irinotecan and Oxaliplatin resistance colon cancer cells and explored the possible signaling pathways involved using In vitro and In vivo models. The results showed that Fisetin treatment effectively inhibited cell viability and apoptosis of CPT11‐LoVo cells than Oxaliplatin (OR) and parental LoVo cancer cells. Western blot assays suggested that apoptosis was induced by fisetin administration, promoting Caspase‐8, and Cytochrome‐C expressions possibly by inhibiting aberrant activation of IGF1R and AKT proteins. Furthermore, fisetin inhibited tumor growth in athymic nude mouse xenograft model. Overall, our results provided a basis for Fisetin as a promising agent to treat parental as well as chemoresistance colon cancer.Accordion content 1.


Bharath Kumar Velmurugan, Chi‐Ru Chan, Ching‐Feng Weng, "Innate‐immune responses of tilapia (Oreochromis mossambicus) exposure to acute cold stress", Journal of cellular physiology, (2019) (ISI, IF: 3.923)



In this present study, Oreochromis mossambicus tilapia were transferred to cold water at 12°C for various time intervals (1, 4, 8, 24, and 48 hr) and its innate immune response was analyzed by studying cellular and humoral parameters. In vivo, alternative complement pathway activity in blood plasma was rapidly increased at 1 hr of cold water (12°C) exposure. Lysozyme activity and cortisol levels of plasma were increased at 4 and 1 hr, respectively. Surprisingly, only plasma cortisol levels remained unchanged through 24 hr of cold water transfer. Phagocytic ability, phagocytic capacity, and respiratory burst (RB) activity of head kidney (HK) leukocytes and splenocytes showed no any significant changes. In peripheral blood leukocytes, phagocytic capacity, and RB activity were increased at 24 hr of cold water exposure. The expressions of genes involved innate immunity in splenocytes and HK leukocytes of tilapia cold water exposure were analyzed, messenger RNA (mRNA) expressions of HSP70, HSP90, and immunoglobulin M failed to change upon exposure to cold stress. Major histocompatibility complex‐I and II mRNAs were significantly increased in tilapia splenocytes at 1 hr of cold water transferred. Whereas myxovirus (Mx) expression was increased in splenocytes and HK leukocytes of tilapia after 1 hr of cold water exposed. Our result reveals that the exposure of tilapia to acute cold stress condition significantly enhances plasma acid phosphatase activity and both phagocytic capacity and RB activity. Furthermore, cold stress significantly stimulates Mx gene expression in splenocytes and HK leukocytes


Tran Thien Khanh, Arko Kesküla, Zane Zondaka, Madis Harjo, Alo Kivilo, Mahdi Safaei Khorram, Tarmo Tamm, Rudolf Kiefer, "Role of polymerization temperature on the performance of polypyrrole/dodecylbenzenesulphonate linear actuators", Synthetic Metals, 247, (2019), 53-58 (ISI, IF: 2.526)



Polypyrrole doped with dodecylbenzenesulphonate (PPy/DBS) free-standing films were electropolymerized in sodium perchlorate propylene carbonate solution at different temperatures, observing increasing conductivity with decreasing synthesis temperature. Our goal in this study was to evaluate how the linear actuation of PPy depends on the polymerization temperature. The anion driven actuation of materials synthesized at lower temperatures changed to mainly cation active for PPy/DBS films polymerized at 20 °C; the latter films also showed the highest diffusion coefficients. Scanning electron microscopy did show that the surface roughness of the films increased with increasing synthesis temperature, as expected. Isometric and isotonic electro-chemo mechanical deformation (ECMD) measurements were performed with combined electrochemical techniques (cyclic voltammetry and chronoamperometry), revealing wide differences in the actuation behavior.


C-Y Huang, BK Velmurugan, M-C Chen, CH Day, W-S Chien, VV Padma, H-C Wu, T-H Lin, H-H Hsu, C-H Shen, "KHC-4 inhibits β-catenin expression in prostate cancer cells", Biotechnic & Histochemistry, (2019), 1-7 (ISI, IF: 1.074)



KHC-4 is a 2-phenyl-4-quinolone analogue that exhibits anticancer activity. Aberrant activation of β-catenin signaling contributes to prostate cancer development and progression. Therefore, targeting β-catenin expression could be a useful approach to treating prostate cancer. We found that KHC-4 can inhibit β-catenin expression and its signaling pathway in DU145 prostate cancer cells. Treatment with KHC-4 decreased total β-catenin expression and concomitantly decreased β-catenin levels in both the cytoplasm and nucleus of cells. KHC-4 treatment also inhibited β-catenin expression and that of its target proteins, PI3K, AKT, GSK3β and TBX3. We monitored the stability of β-catenin with the proteasomal inhibitor, MG132, in DU145 cells and found that MG132 reversed KHC-4-induced proteasomal β-catenin degradation. We verified CDK1/β-catenin expression in KHC-4 treated DU145 cells. We found that roscovitine treatment reversed cell proliferation by arresting the cell cycle at the G2/M phase and β-catenin expression caused by KHC-4 treatment. We suggest that KHC-4 inhibits β-catenin signaling in DU145 prostate cancer cells


Thai Minh Duy Le, Huu Thuy Trang Duong, Thavasyappan Thambi, VH Giang Phan, Ji Hoon Jeong, Doo Sung Lee, "Bioinspired pH-and Temperature-Responsive Injectable Adhesive Hydrogels with Polyplexes Promotes Skin Wound Healing", Biomacromolecules, 19, 8, (2018), 3536-3548 (ISI, IF: 5.738)


Despite great potential, the delivery of genetic materials into cells or tissues of interest remains challenging owing to their susceptibility to nuclease degradation, lack of permeability to the cell membrane, and short in vivo half-life, which severely restrict their widespread use in therapeutics. To surmount these shortcomings, we developed a bioinspired in situ-forming pH- and temperature-sensitive injectable hydrogel depot that could control the delivery of DNA-bearing polyplexes for versatile biomedical applications. A series of multiblock copolymer, comprised of water-soluble poly(ethylene glycol) (PEG) and pH- and temperature-responsive poly(sulfamethazine ester urethane) (PSMEU), has been synthesized as in situ-forming injectable hydrogelators. The free-flowing PEG–PSMEU copolymer sols at high pH and room temperature (pH 8.5, 23 °C) were transformed to stable gel at the body condition (pH 7.4, 37 °C). Physical and mechanical properties of hydrogels, including their degradation rate and viscosity, are elegantly controlled by varying the composition of urethane ester units. Subcutaneous administration of free-flowing PEG–PSMEU copolymer sols to the dorsal region of Sprague–Dawley rats instantly formed hydrogel depot. The degradation of the hydrogel depot was slow at the beginning and found to be bioresorbable after two months. Cationic protein or DNA-bearing polyplex-loaded PEG–PSMEU copolymer sols formed stable gel and controlled its release over 10 days in vivo. Owing to the presence of urethane linkages, the PEG–PSMEU possesses excellent adhesion strength to wide range of surfaces including glass, plastic, and fresh organs. More importantly, the hydrogels effectively adhered on human skin and peeled easily without eliciting an inflammatory response. Subcutaneous implantation of PEG–PSMEU copolymer sols effectively sealed the ruptured skin, which accelerated the wound healing process as observed by the skin appendage morphogenesis. The bioinspired in situ-forming pH- and temperature-sensitive injectable adhesive hydrogel may provide a promising platform for myriad biomedical applications as controlled delivery vehicle, adhesive, and tissue regeneration

Huu Thuy Trang Duong, Yue Yin, Thavasyappan Thambi, Thanh Loc Nguyen, VH Giang Phan, Min Sang Lee, Jung Eun Lee, Jaeyun Kim, Ji Hoon Jeong, Doo Sung Lee, "Smart vaccine delivery based on microneedle arrays decorated with ultra-pH-responsive copolymers for cancer immunotherapy", Biomaterials, 185, (2018), 13-24 (ISI, IF: 8.806)



Despite the tremendous potential of DNA-based cancer vaccines, their efficacious delivery to antigen presenting cells to stimulate both humoral and cellular response remains a major challenge. Although electroporation-based transfection has improved performance, an optimal strategy for safe and pain-free vaccination technique remains elusive. Herein, we report a smart DNA vaccine delivery system in which nanoengineered DNA vaccine was laden on microneedles (MNs) assembled with layer-by-layer coating of ultra-pH-responsive OSM-(PEG-PAEU) and immunostimulatory adjuvant poly(I:C), a synthetic double stranded RNA. Transcutaneous application of MN patches onto the mice skin perforate the stratum corneum with minimal cell damage; subsequent disassembly at the immune-cell-rich epidermis/dermis allows the release of adjuvants and DNA vaccines, owing to the ultra-sharp pH-responsive nature of OSM-(PEG-PAEU). The released adjuvant and DNA vaccine can enhance dendritic cell maturation and induce type I interferons, and thereby produce antigen-specific antibody that can achieve the antibody-dependent cell-mediated cytotoxicity (ADCC) and CD8+ T cell to kill cancer cells. Strikingly, transcutaneous application of smart vaccine formulation in mice elicited 3-fold greater frequencies of Anti-OVA IgG1 serum antibody and 3-fold excess of cytotoxic CD8+ T cell than soluble DNA vaccine formulation. As a consequence, the formulation rejected the murine B16/OVA melanoma tumors in C57BL/6 mice through the synergistic activation of antigen-specific ADCC and cytotoxic CD8+ T cells. The maneuvered use of vaccine and adjuvant poly(I:C) in MNs induces humoral and cellular immunity, which provides a promising vaccine technology that shows improved efficacy, compliance, and safety


Siti Norayu Idris, Abdul Bakrudeen Ali Ahmed and Rosna Mat Taha, “Sucrose enhanced stigmasterol production in callus cultures of Wedelia biflora (L.) D.C.”, Philippine Agricultural Scientist, 2018, 101, 3, 251-260 (ISI, IF: 0.27).


Callus culture method was used to extract stigmasterol in mass amount. The current study aims to enhance the production of stigmasterol by manipulation of sucrose concentrations in Murashige and Skoog (MS) media. A combination of 2 mg NAA L-1 (an auxin) and 2.5 mg 6-benzylaminopurine (BAP) L-1 (a cytokinin) in MS medium was the best concentration to stimulate callus formation from stem explants of W. biflora with the fastest response (10 d), the highest biomass of callus (55 mg dry weight) and the best callus characteristic (green and compact). Application of 4% (w/v) sucrose in MS medium enhanced callus formation along with callus biomass and stigmasterol production (88 mg g-1) compared with the control (58.3 mg g-1) and in vivo grown stem (53.4 mg g-1). Production of stigmasterol from W. biflora with reduced production time and cost is now possible with the methods obtained from the current study.


A.B.A. Ahmed, J. Magesh, S.N. Idris, E.N. Mubarak and R.M. Taha, “Chemical Constituents and Antimicrobial Activity of Essential Oils from Micropropagation and Field-Grown Plants ofWedelia biflora [L.]”, Journal of essential oil bearing plants, 2018, 21, 6, 1712-1724 (ISI: IF 0.72)


An efficient regeneration protocol through axillary and inter nodal explants was developed from Wedelia biflora. Explants were placed on MS medium supplemented with different treatments of cytokinins, 6-benzyladenine (BA) and kinetin (KIN) for adventitious shoot production. The highest multiple shoots (95 %; 26.4 shoots/ explant) and elongation (2.75 cm shoot length), was obtained in 1.0 BA mg L-1 in axillary node explants. The shoot was transferred to half-strength MS medium supplemented with 2.0 mg L-1 IBA showed the best root formation (85 %; 22.2 roots/shoot; 2.65 cm root length; propagate leaf d.w 1.42 g L-1 and root d.w 1.10 g L-1) and plantlets were successfully acclimatized in the green house. The compositions of essential oils were studied in in vitro and in vivo leaf explants and to determine their absolute and relative concentrations using GC-MS and GC-FID, respectively. In vitro and in vivo variations in composition among the essential oils were detected. Sesquiterpene and monoterpene are major components in the oil of in vitro and in vivo leaves of W. biflora. Essential oil quantification showed α-pinene (44.03 %); verbenone (15.37 %); δ-cadinene (5.48 %) of in vivo leaves; whereas α-pinene (5.02 %); verbenone (1.40 %) and δ-cadinene (3.13 %) of in vitroleave respectively. The in vitro and in vivo essential oils were analyzed by bacterial and fungal organism through disc diffusion and minimum inhibitor concentration (MIC) methods. The results were compared with reference drugs ciprofloxacin and nystatin respectively. In vitro developed plants exhibited higher sesquiterpene contents and antimicrobial activity than field grown plant.


Bharath Kumar Velmurugan, Baskaran Rathinasamy, Bharathi Priya Lohanathan, Varadharajan Thiyagarajan and Ching-Feng Weng, “Neuroprotective Role of Phytochemicals”, Molecules, 2018, 23 (10), 2485 (ISI: IF: 3.060)


Neurodegenerative diseases are normally distinguished as disorders with loss of neurons. Various compounds are being tested to treat neurodegenerative diseases (NDs) but they possess solitary symptomatic advantages with numerous side effects. Accumulative studies have been conducted to validate the benefit of phytochemicals to treat neurodegenerative diseases including Alzheimer’s disease (AD) and Parkinson’s disease (PD). In this present review we explored the potential efficacy of phytochemicals such as epigallocatechin-3-galate, berberin, curcumin, resveratrol, quercetin and limonoids against the most common NDs, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). The beneficial potentials of these phytochemicals have been demonstrated by evidence-based but more extensive investigation needs to be conducted for reducing the progression of AD and PD.


M.S. Khorram, G. Zhang, A. Fatemi, R. Kiefer, K. Maddah, M. Baqar, Md P. Zakaria and G. Li, “Impact of biochar and compost amendment on soil quality, growth and yield of a replanted apple orchard in a 4-year field study”, Journal of the Science of Food and Agriculture, 2019, 99, 1862-1869 (ISI, h index 121, IF 2.42).


Numerous studies have addressed the positive effects of organic amendments on soil and plant productivity under short‐term field studies. However, to date, few studies have been conducted on the effects of organic amendment on the orchards where high nutrient bioavailability is required. This study deals with the effects of biochar and compost on soil quality, growth and yield of a replanted apple orchard in the northeast of Iran.
Biochar+compost application resulted in 37% and 300% higher soil total organic carbon and available phosphorus content, respectively, during the first 3 years of experimentation compared to control. Similarly, trunk diameter and shoot number of apple trees increased 23–26% by the end of the first year. Nevertheless, there were no significant changes in fruitfulness, fruit weight or starch pattern index as productivity indices.
Biochar and compost were beneficial in improving soil quality, mainly by increasing soil nutrient content and decreasing soil bulk density, and in increasing plant growth at early growth stages of apple orchards. However, they failed to enhance overall yield and fruit quality, most likely due to their limited ability to suppress apple replant disease.


I. Põldsalu, U. Johanson, T. Tamm, A. Punning, F. Greco, A.-L. Peikolainen, R. Kiefer, A. Aabloo, “Mechanical and electro-mechanical properties of EAP actuators with inkjet printed electrodes”, Synthetic Metals, 2018, 246, 122–127 (ISI, h index 132, IF 2.9).


Electrically conductive polymer (CP) based ionic electromechanically active polymer composites (IEAP-s) are attractive as bending and linear actuators in compliant and miniature devices due to low operating voltage. Ink-jet printing is a promising technology for fabrication of microscale CP-based IEAP-s with customized shapes and geometries. The current study investigates tailoring of the mechanical and electromechanical properties of the actuators by controlled growth of ink-jet printed poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS) based electrodes on commercial poly(vinylidene fluoride) (PVdF) membranes. In parallel with PEDOT:PSS, hybrid actuators with ink-jet printed PEDOT:PSS and activated carbon aerogel electrodes were investigated. Cumulative growth of electrodes with each deposited layer was achieved in the case of both electrode materials. The strain, blocking force and capacitance of the actuators were in linear correlation with the thickness of the electrodes. Simple method of control encourages implementation of ink-jet-printing technology for manufacturing of IEAP micro-actuators with desired mechanical and electromechanical properties.


M.Tania, J. Shawon, K. Saif, R. Kiefer, M. Safaei Khorram, Md. A. Halim and Md. A. Khan, “Cordycepin Downregulates Cdk-2 to Interfere with Cell Cycle and Increases Apoptosis by Generating ROS in Cervical Cancer Cells: in vitro and in silico Study”, Current Cancer Drug Targets, 2019, 19, 152-159 (ISI, H index 85, IF: 2.28).


Background: Cordycepin is a small molecule from medicinal mushroom Cordyceps, which has been reported for anticancer properties.
Objective: In this study, we have focused on the investigation of cordycepin effect on cervical cancer cells with further clarification of possible molecular mechanism. 
Method: We have used cell viability and cell counting assay for cytotoxic effect of cordycepin, flow cytometric assay of apoptosis and cell cycle, and quantitative PCR (qPCR) and Western blotting for the determination of target gene expression. Molecular docking and Molecular dynamics simulation were used for in silico analysis of cordycepin affinity to target protein(s). 
Results: Treatment of cordycepin controlled SiHa and HeLa cervical cancer cell growth, increased the rate of their apoptosis, and interfered with cell cycle, specifically elongated S-phase. qPCR results indicated that there was a downregulation of cell cycle proteins CDK-2, CYCLIN-A2 and CYCLIN-E1 in mRNA level by cordycepin treatment but no significant change was observed in pro-apoptotic or antiapoptotic proteins. The intracellular reactive oxygen species (ROS) level in cordycepin treated cells was increased significantly, implying that apoptosis might be induced by ROS. Western blot analysis confirmed significant decrease of Cdk-2 and mild decrease of Cyclin-E1 and Cyclin-A2 by cordycepin, which might be responsible for regulating cell cycle. Molecular docking indicated high binding affinity of cordycepin against Cdk-2. Molecular dynamics simulation further confirmed that the docked pose of cordycepin-Cdk-2 complex remained within the binding pocket for 10 ns. 
Conclusion: Our study suggests that cordycepin is effective against cervical cancer cells, and regulating cell cycle via cell cycle proteins, especially downregulating Cdk-2, and inducing apoptosis by generating ROS are among the mechanisms of anticancer activities of cordycepin.


R. Kiefer, R. Khadka, J. Travas-Sejdic, “Poly(ethylene oxide) in polypyrrole doped dodecylbenzenesulfonate: characterisation and linear actuation”, International Journal of Nanotechnology, 2018, 15, 689-694 (ISI, H index 32, IF 0.56).


We show improvement in linear actuation properties of polypyrrole-doped dodecylbenzenesulfonate (PPy/DBS) by incorporating varying amounts of polyethylene oxide (5 wt % or 15 wt %) into PPy during electroploymerisation (PPy/DBS-PEO). Knowing that pristine PPy/DBS linear actuators in aqueous electrolytes are driven by cation ingress during reduction, the actuation properties are studied in isotonic (length change) mode of electro-chemo-mechanical-deformation (ECMD) measurements. Scanning electronic microscopy (SEM) and energy dispersive X-ray (EDX) spectroscopy revealed that PEO is incorporated in PPy/DBS films. ECMD studies revealed a three-fold improvement in electronic conductivity for the PPyDBS-PEO 15% film that also demonstrated improved strain of 7% in comparison to the strain of PPy/DBS film of 1.9%.


Z. Zondaka, T. Tamm, A. Aabloo, R. Kiefer, "Solvent change in polymerization influence linear actuation of polypyrrole carbide-derived carbon films ," Proceedings SPIE 10594, Electroactive Polymer Actuators and Devices (EAPAD) XX, 105942H (27 March 2018) (conference scopus, h index 151, IF 0.56)


For actuator applications, conducting polymers are typically deposited at lower temperatures e.g. -20°C, in order to obtain more regular, smooth and ordered films with fewer defects. Clearly, standard aqueous electrolyte solutions cannot be used at these temperatures, and some organic solvents cannot be used, as not all salts are soluble in these. A wellknown approach is to use ethylene glycol: water mixtures (often 50:50 wt%, PPy/CDC(EG:W). The goal of this work is to analyze the role of water in the solution: to compare the linear actuation properties of conducting polymer hybrid films PPy/CDC(EG) polymerized from just ethylene glycol solution and that with 50% water, both at -20°C. Cyclic voltammetry and chronoamperometric electro-chemo-mechanical-deformation (ECMD) measurements were performed to study the linear actuation properties in lithium bis(trifluoromethylsulfonyl)imide in aqueous solution (LiTFSI-aq). Both conductivity and linear actuation strain were found dependent on the solvent in the polymerization solution with ethylene glycol: water the clearly superior solvent choice.


A. Kivilo, Z. Zondaka, S. Nakshatharan, K.-A. Küppar, T. Tamm, R. Kiefer, "Poly-3,4-ethylenedixoythiophene on carbide-derived carbon trilayer: combined linear actuation characterization", Proc. SPIE 10594, Electroactive Polymer Actuators and Devices (EAPAD) XX, 105942L (27 March 2018) (conference scopus, h index 151, IF 0.56)


Carbide-derived carbon (CDC) based actuators have been typically formulated as trilayer systems and applied in bending displacement. In this work, we want to demonstrate that CDC deposited on glass fiber fabric (CDC-TL) with an additional poly-3.4-ethylenedioxythiophene (PEDOT) layer electropolymerized on top forming a polylayer (expressed as CDC-PEDOT-TL) can be used as a linear actuator. Isotonic and isometric electro-chemo-mechanical deformation (ECMD) measurements in lithium bis(trifluoromethane) sulfonamide propylene carbonate (LiTFSI-PC) were performed, revealing that the CDC expansion at discharging can be found in CDC-PEDOT-TL (main expansion at oxidation of 0.5% strain) in same extent of 0.24 %. The stress found in similar values of 30 kPa for both system. Besides the nearly 5times better conductivity of CDC-PEDOT-TL, the charge density reduce nearly half in comparison to CDC-TL.


Dieu-Hien Truong, Dinh Hieu Nguyen, Nhat Thuy Anh Ta, Anh Vo Bui, Tuong Ha Do and Hoang Chinh Nguyen, “Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia”, Journal of Food Quality, 2019, 8178294 (ISI: IF: 1,767).


Severinia buxifolia (Rutaceae) is a promising source of bioactive compounds since it has been traditionally used for the treatment of various diseases. The present study aimed at evaluating the impact of different solvents on extraction yields, phytochemical constituents and antioxidants, and in vitro anti-inflammatory activities of S. buxifolia. The results showed that the used solvents took an important role in the yield of extraction, the content of chemical components, and the tested biological activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w). The extract obtained from methanol exhibited high capacity of antioxidant (IC50value of 16.99 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 28.86 μg/mL; antiproteinase activity: IC50 = 414.29 μg/mL; and membrane stabilization: IC50 = 319 μg/mL). The antioxidant activity of the S. buxifolia extract was found to be 3-fold higher than ascorbic acid, and the anti-inflammatory activity of S. buxifolia extract was comparable to aspirin. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy.


Nguyen Chi Thanh, Boonchai Wichitsathian, Chatpet Yossapol, Watcharapol Wonglertarak and Borano Te, “Improvement of aqueous solution coexisting with arsenite and arsenate using iron mixed porous clay pellets in batch and fixed-bed column studies”, Water Science and Technology: Water Supply, 2019, ws2019069 (ISI: IF: 0.674).


Arsenic-polluted water is a global concern and puts millions of people at risk of developing cancer. The improvement of aqueous solution coexisting with arsenite and arsenate using iron mixed porous clay pellets was investigated in batch and fixed-bed column systems. Batch studies showed that the removal rate occurred in two main phases with an equilibrium time of 52 h. The pseudo-second-order model well described the experimental data. Isotherm data were well fitted by the Langmuir–Freundlich model. The removal efficiency was significantly reduced in alkaline solution and the presence of phosphate ions. The column study revealed that the breakthrough time and saturation time increased with lower feeding flow rate, higher bed height, and lower initial adsorbate concentration. The Thomas model provided good performance for predicting the column experimental data.


Nagarajan Ganesan, Rathinasamy Baskaran, Bharath Kumar Velmurugan and Nguyen Chi Thanh, “Antrodia cinnamomea—An updated minireview of its bioactive components and biological activity”, Journal of Food Biochemistry, 2019, 43, e12936 (ISI: IF: 1.552).


Antrodia cinnamomea or Antrodia camphorata is a distinctive mushroom of Taiwan, which is being used as a traditional medicine to treat various health‐related conditions. More than 78 compounds have been identified in A. cinnamomea. Large numbers of phytochemical studies have been carried out in A. cinnamomea due to the high amount of terpenoids. Besides that, the extracts and active components of A. cinnamomea were reported to have various biological activities including hepatoprotective, antihypertensive, antihyperlipidemic, anti‐inflammatory, antioxidant, antitumor, and immunomodulatory activities. In this review article, we have summarized the recent findings of A. cinnamomea and its molecular mechanisms of action in various disease models.


Minh-Khiem Nguyen, Kuan-Hung Lin, Li-Ru Chen, Tung-Chuan Hsiung and Yukio Ozaki, “Efficient in vitro Plant Regeneration from Protocorm of Bletilla formosana (Hayata) Schltr.”, Journal of the Faculty of Agriculture, Kyushu University, 2018, 63, 2, 231–240 (ISI: IF: 0.351).


An efficient mass propagation method for Bletilla formosana (Hayata) Schltr. was successfully established through direct shoot organogenesis. Multiple shoots were induced from protocorm explants on half– strength Murashige and Skoog (1/2–MS) basal medium containing 2 mg/L N–phenyl–N’–1,2,3–thiadiazol–5– yl urea (TDZ) and 0.5 mg/L 1–naphthaleneacetic acid (NAA) with the highest shoot formation rate of 100% and a maximum average shoot number of 5.2. The application of either NAA or 2,4–dichlorophenoxy (2,4–D) significantly induced root induction from shoots, and 2 mg/L NAA provided the highest root formation rate of 100% and a maximum number of roots (4.4 per shoot). Supplementation with kinetin and benzylaminopurine (BAP) of 0.5~2.0 mg/L in 1/2–MS basal medium significantly promoted plantlet growth. To optimize the in vitro development of plantlets, 1/2–MS basal medium with modified 1/4–strength nitrogen content, and 20 g/L sucrose was used. Well–developed plantlets were successfully acclimatized in a greenhouse with over a 90% survival rate. This effective protocol of in vitro plant regeneration through direct shoot organogenesis can be utilized for the mass propagation and germplasm conservation of B. formosana.


Kuo-Chan Hung, Ngoc Tuan Nguyen, Yu-Ling Sun and Shir-Ly Huang, “Bio-Fenton reaction involved in the cleavage of the ethoxylate chain of nonionic surfactants by dihydrolipoamide dehydrogenase from Pseudomonas nitroreducens TX1”, Scientific Reports, 2019, 9, 6827 (ISI: IF: 4.122)


Bacteria in the environment play a major role in the degradation of widely used man-made recalcitrant organic compounds. Pseudomonas nitroreducens TX1 is of special interest because of its high efficiency to remove nonionic ethoxylated surfactants. In this study, a novel approach was demonstrated by a bacterial enzyme involved in the formation of radicals to attack ethoxylated surfactants. The dihydrolipoamide dehydrogenase was purified from the crude extract of strain TX1 by using octylphenol polyethoxylate (OPEOn) as substrate. The extent of removal of OPEOs during the degradation process was conducted by purified recombinant enzyme from E. coli BL21 (DE3) in the presence of the excess of metal mixtures (Mn2+, Mg2+, Zn2+, and Cu2+). The metabolites and the degradation rates were analyzed and determined by liquid chromatography-mass spectrometry. The enzyme was demonstrated to form Fenton reagent in the presence of an excess of metals. Under this in vitro condition, it was shown to be able to shorten the ethoxylate chains of OPEOn. After 2 hours of reaction, the products obtained from the degradation experiment revealed a prominent ion peak at m/z = 493.3, namely the ethoxylate chain unit is 6 (OPEO6) compared to OPEO9 (m/z = 625.3), the main undegraded surfactant in the no enzyme control. It revealed that the concentration of OPEO15 and OPEO9 decreased by 90% and 40% after 4 hours, respectively. The disappearance rates for the OPEOn homologs correlated to the length of the exothylate chains, suggesting it is not a specific enzymatic reaction which cleaves one unit by unit from the end of the ethoxylate chain. The results indicate the diverse and novel strategy by bacteria to catabolize organic compounds by using existing housekeeping enzyme(s).


Le Quoc Bao, Vu Hoang Giang Phan and Nguyen Quang Khuyen, “Investigating effects of nano cerium oxide reinforcement on mechanical properties of composite based on natural rubber”, AIP Conference Proceedings, 2018, 1954, 1, 10.1063/1.5033391 (conference scopus, h index 54)


Polymer nanocomposites that based on combination of nanomaterials (such as nanoparticles, nanotubes, nanorods, nanofibers, and nanosheets) and polymeric matrices are receiving great attention in research and application. However, separate and homogenous dispersion rather than aggregates of nanoparticles into matrices meet big difficulty due to large interaction between nanoparticles. The poor dispersion leads to low properties of nanocomposites. In this study, we find out the appropriate method to separately disperse cerium oxides (CeO2) nanoparticles into natural rubber, aiming to increase mechanical properties of natural rubber. The SEM images were used to evaluate the dispersion of nano CeO2 in natural rubber matrix. The mechanical properties of nanocomposites were measured after vulcanization to investigate effects of nano CeO2amount on prepared composite. The findings exhibited that the addition of CeO2 by dispersion of nano CeO2 in water via ultrasonication before mixing with rubber latex, significantly increase modulus, tear and wear resistance of natural rubber. 


Hoang Chinh Nguyen, Dinh Thi My Huong, Horng-Yi Juan, Chia-Hung Su and Chien-Chung Chien, “Liquid Lipase-Catalyzed Esterification of Oleic Acid with Methanol for Biodiesel Production in the Presence of Superabsorbent Polymer: Optimization by Using Response Surface Methodology”, Energies, 2018, 11, 5, 1085 (ISI: IF 2.676)


Liquid lipase-catalyzed esterification of fatty acids with methanol is a promising process for biodiesel production. However, water by-product from this process favors the reverse reaction, thus reducing the reaction yield. To address this, superabsorbent polymer (SAP) was used as a water-removal agent in the esterification in this study. SAP significantly enhanced the conversion yield compared with the reaction without SAP. The lipase-catalyzed esterification in the presence of SAP was then optimized by response surface methodology to maximize the reaction conversion. A maximum conversion of 96.73% was obtained at a temperature of 35.25 °C, methanol to oleic acid molar ratio of 3.44:1, SAP loading of 10.55%, and enzyme loading of 11.98%. Under these conditions, the Eversa Transform lipase could only be reused once. This study suggests that the liquid lipase-catalyzed esterification of fatty acids using SAP as a water-removal agent is an efficient process for producing biodiesel.


Rishabh Upadhyay, Soeren Steudel, Mai-Phi Hung, Akhilesh Kumar Mandal, Francky Catthoor and Manoj Nag, “Self-Aligned Amorphous Indium-Tin-Zinc-Oxide Thin Film Transistors on Polyimide Foil”, ECS Journal of Solid State Science and Technology, 2018, 7, 4, 185-191 (ISI: IF 1.808)


In this work, we report on high-performance coplanar self-aligned (SA) amorphous-Indium-Tin-Zinc-Oxide (a-ITZO) thin-film transistors (TFTs) on flexible polyimide substrate. The a-ITZO films are first optimized with respect to the oxygen ratio, thickness and final anneal conditions with common-gate TFTs structure on Si/SiO2substrate. Optimized TFTs show mobility (μlin) between 20.0–25.0 cm2/(V.s). Material characterization revealed some degree of order compared to a truly amorphous film like a-IGZO but no grain boundaries or crystalline domains were observed. The a-ITZO films were integrated in coplanar SA TFT architecture on polyimide using hydrogen rich plasma (SiH4 based chemistry) as dopant for the source/drain (S/D) regions resulting in field-effect mobility (μFE) of 27.0 cm2/ (V.s), sub-threshold slope (SS−1) of 0.40 V/decade and ION /IOFF ratio of >108. The threshold voltage shifts of the TFTs under both positive and negative gate bias stress of 1MV/cm for 104 seconds were less than 1.5 V. We have also investigated the applicability of the SA a-ITZO TFTs in logic circuitry such as 19-stage ring-oscillators (ROs).


Mai Phi Hung, Christopher Chare, Manoj Nag, A. de Jamblinne de Meux, Jan Genoe and Soere Steudel, “Effect of High Oxygen Partial Pressure on Carrier Transport Mechanism in a-InGaZnO TFTs”, IEEE Transactions on Electron Devices, 2018, 65, 7, 2833-2837 (ISI: IF 2.605)


In this paper, the influence of oxygen partial pressure (P O2 ) during physical vapor deposition on the field-effect mobility of amorphous InGaZnO (IGZO) thin-film transistor (TFT) is investigated in a wide range of P O2 . The field-effect mobility reduced from 12 to 0.1 cm 2 /V · s when the P O2 is increased from 0.3 to 1.5 mTorr. Temperature-dependent TFT measurements suggest that carrier transport in the TFTs deposited at the P O2 of 0.3 mTorr can be described according to the multiple trap and release/percolation model. However, the carrier transport in the TFTs deposited at the P O2 of 1.5 mTorr conforms to Mott's variable-range hopping model. The elastic recoil detection analysis indicates that hydrogen concentration in the IGZO film increased with P O2 . The X-ray photoelectron spectroscopy results indicate that In vacancy (V In ) defects formed in the IGZO film deposited at high P O2 . Scattering induced by hydrogen and pinning of Fermi level below the conduction band edge by V In are plausible reasons for the low field-effect mobility in TFTs deposited at high P O2 .


Mai Phi Hung, Jan Genoe, P. Heremans, and Soere Steudel, “Off-current reduction in p-type SnO thin film transistors”, Applied Physics Letters, 2018, 112, 26, 263502 (ISI: IF 3.495)



SnO is one of the few candidates for p-type oxide thin film transistors (TFTs) because it retains a reasonable high hole mobility in a nanocrystalline film. However, the high off-current of SnO TFT limits its usefulness. In this work, SnO TFTs were fabricated using thermal evaporation under ultra-high vacuum. In order to decrease the off-current in p-type SnO thin film transistors (TFTs), we used yttrium to reduce n-type minority charges in the channel. The on/off ratio of the TFT increases from 102 to 5 × 104 and the mobility of the TFT in the saturated regime reduces from 1.6 to 1.4 cm2/V s when the SnO channel is doped with 1 wt. % of Y. Grazing incidence X-ray diffraction and X-ray photoelectron spectroscopy reveal that the reduction of SnO2 in the Y-doped SnO TFT channel is the main reason for the improvement in the TFT characteristics.

Publication 2017 – 2018

1 Bharath Kumar Velmurugan, Ruey‐Lin Chang, Shibu Marthandam Asokan, Chih‐Fen Chang, Cecilia‐Hsuan Day, Yueh‐Min Lin  Yuan‐Chuan Lin, Wei‐Wen Kuo, Chih‐Yang Huang, “A minireview of E4BP4/NFIL3 in heart failure”, Journal of Cellular Physiology, (2018), doi: 10.1002/jcp.26790
2 Ngoc Tuan Nguyen, “Acinetobacter soli SP2 capable of high-efficiency degradation of food emulsifier polysorbate 80”, Current Microbiology, 75, 7, (2018), 896-900
3 Luong Thu-Hien, Nguyen Thanh-Truc, Vo Van Toi, Huynh Chan Khon, Bui Chi Bao, Vo Van Thanh Niem, Mai Ngoc Tuan Anh, Nguyen Dai Hai, Pham Dinh Chuong, Nguyen Thi Hiep, “Evaluation of the morphology and biocompatibility of natural silk fibers/agar blend scaffolds for tissue regeneration”, Hindawi International Journal of Polymer Science, (2018), Article ID 5049728 (7 pages)
4 Rathinasamy Baskaran, Bharath KumarVelmurugan, “Role of lncRNAs in the cancer development and progression and their regulation by various phytochemicals”, Biomedicine & Pharmacotherapy, 102, (2018), 242-248
5 Pi-Yu Chao, Meng-Yuan Huang, Wen-Dar Huang, Kuan-Hung Lin, Shiau-Ying Chen, Chi-Ming Yang, “Study of chlorophyll-related compounds from dietary spinach in human blood”, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46, 2, (2018), 309-316
6 Mong-Huai Su, Te-Hua Hsu, Chun-Neng Wang, Kuan-Hung Lin, Ming-Chang Chiang, Ren-Dong Kang, Khiem Minh Nguyen, Hoang Nguyen, “Genetic diversity of a novel oil crop, camellia brevistyla, revealed by ISSR DNA markers”, Horticultural Science and Technology, 35, 5, (2017), 588-598*/N0130350507.pdf?_=3&authcred=anVzZXJfY3Jvc3NkYjpKb3VybmFsKTk4
7 Kuan-Hung Lin, Penk-Yeir Low, Pi-Yu Chao, Ming-Chih Shih, Ming-Chang Chiang, Yung-Chang Lai, Swi-Bea Wu, “Antioxidant properties and glucose uptake effect of ethanol extracts from different sweet potato leaves prepared by lyophilization and oven-drying at 40 °C”, Current Nutrition & Food Science, 13, 3, (2017), 227-236


No Authors
1 Hoang Chinh Nguyen, Kuan-Hung Lin, Meng-Yuan Huang, Chi-Ming Yang, Tin-Han Shih, Tung-Chuan Hsiung, Yen-Chang Lin, Fun-Chi Tsao, Antioxidant Activities of the Methanol Extracts of Various Parts of Phalaenopsis Orchids with White, Yellow, and Purple Flowers, Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2018, 46(2), 457–465.


No Authors
1 Hoang Chinh Nguyen, Szu-Pei Wu, Chia-Hung Su, Tzann-Shun Hwang, Biochemical, biophysical, and thermal properties of alkaline phosphatase from thermophile Thermus sp. NTU-237, Biotechnology, Agronomy, Society, Environment. 2017 21(2), 117–126(ISI, IF: 0.433). 
2 Hoang Chinh Nguyen, Kuan-Hung Lin, Tung-Chuan Hsiung, Meng-Yuan Huang, Chi-Ming Yang, Jen-Hsien Weng, Ming-Huang Hsu, Po-Yen Chen and Kai-Chieh Chang, Biochemical and Physiological Characteristics of Photosynthesis in Plants of Two Calathea Species, International Journal of Molecular Sciences, 2018, 19(3), 704(ISI, IF: 3.226).


No Authors
1 Marthandam Asokan Shibu, M. Ravichandran, M.Shanmugavadivu, Bharath Kumar Velmurugan, Pharmacological benefits of Neferine - a comprehensive review, Life Sciences, 2018, 199, 60–70(ISI, IF: 2.936).


No Authors
1 Thai Thanh Hoang Thi, Yunki Lee, Phuong Le Thi, Ki Dong Park, Nitric oxide-releasing injectable hydrogels with high antibacterial activity through in situ formation of peroxynitrite, Acta Biomaterialia, 2018, 67, 66–78(ISI, IF: 6.319).
2 Helene Kjær Risinggård, Simon Cooil, Federico Mazzola, Di Hu, Marit Kjærvik, Elise Ramleth Østli, Nilesh Patil, Alexei Preobrajenski, D. Andrew Evans, Dag W. Breiby, Thuat T. Trinh, Justin W. Wells, Degradation of the chemotherapy drug 5-fluorouracil on medical-grade silver surfaces, Applied Surface Science, 2018, 435, 1213–1219(ISI, IF: 3.387).


No Authors
1 Inga Põldsalu, Kätlin Rohtlaid, Tran Minh Giao Nguyen, Cedric Plesse, Frédéric Vidal, Mahdi Safaei Khorram, Anna-Liisa Peikolainen, Tarmo Tamm, Rudolf Kiefer, Thin ink-jet printed trilayer actuators composed of PEDOT:PSS oninterpenetrating polymer networks, Sensors and Actuators B: Chemical, 2018, 258, 1072–1079 (ISI, IF: 5.401).


No Authors
1 Roshan Khadka, Nihan Aydemir, Arko Kesküla, Tarmo Tamm, Jadranka Travas-Sejdic, Rudolf Kief, Enhancement of polypyrrole linear actuation with poly(ethylene oxide), Synthetic Metals, 2017, 232, 1–7(ISI, IF: 2.435).
2 Madis Harjo, Arko Kesküla, Kaur Leemets, Mahdi Safaei Khorram, Rando Saar,  Martin Järvekülg, Tarmo Tamm, Rudolf Kiefer, Polypyrrole coatings on gelatin fiber scaffolds: Material and electrochemical characterizations in organic and aqueous electrolyte, Synthetic Metals, 2017, 232, 25–30  (ISI, IF: 2.435).
3 Tuan Tran Trong, Dieu-Hien Truong, Dieu-Thai Tran, Huyen-Trang Nguyen Thi, Giap Do Dang, Ho Nguyen Huu, Biomass accumulation of Panax vietnamensis in cell suspension cultures varies with addition of plant growth regulators and organic additives, Asian Pacific Journal of Tropical Medicine,2017,10(9), 907–915  (Scopus).


No Authors
1 T. M. Ha Vuong, Didier Villemin, Hung-Huy Nguyen, Tin Thanh Le, Tung T. Dang, Hien Nguyen, Programmed Site-Selective Palladium-Catalyzed Arylation of Thieno[3,2-b]thiophene, Chemistry An Asian Journal, 2017, 12(21), 2819–2826 (ISI, IF: 4.083).
2 Le Quoc Bao, Phuong Ho, Ramesh Kumar Chitumalla, Joonkyung Jang, Suresh Thogiti, Jae Hong Kim, Single and double branched organic dyes based on carbazole and red-absorbing cationic indolium for p-type dye-sensitized solar cells: A combined experimental and theoretical investigation, Dyes and Pigments,2018, 149, 25–36 (ISI, IF: 3.473).
3 Thanh M. Nguyen, Senaratne L. Ranamukhaarachchi and Phuc D. A. Nguyen, Pesticide use and health hazards among small-scale commercial vegetable growers in the central highland region of Vietnam, Research on Crops, 2017, 18 (3), 497–507 (Scopus). 


No Authors
1 Thien-KhanhTran, Kuo-FengChiu, Chiu-YueLin, Hoang-JyhLeu, Electrochemical treatment of wastewater: selectivity of the heavy metals removal process, International journal of hydrogen energy, 2017, 42(45), 27741–27748 (ISI, IF: 3.313). 
2 Bharath Kumar Velmurugan, Chien-Hung Lee, Shang-Lun Chiang, Chun-Hung Hua, Mei-Chung Chen, Shu-Hui Lin, Kun-Tu Yeh, Ying-Chin Ko, PP2A deactivation is a common event in oral cancer and reactivation by FTY720 shows promising therapeutic potential, Journal of Cellular Physiology, 2017, 233(2), 1300–1311 (ISI, IF: 3.839).
3 Meng-Yuan Huang, Kuan-Hung Lin, Chien-Chun Lu, Li-Ru Chen, Tung-Chuan Hsiung, Wen-Ting Chang, the intensity of blue light-emitting diodes influences the antioxidant properties and sugar content of oyster mushrooms (Lentinus sajor-caju), Scientia Horticulturae, 2017, 218, 8–13 (ISI, IF: 1.624).
4 Yu-Duan Liao, Kuan-Hung Lin, Chiu-Chen Chen, Chih Ming Chiang, Oryza sativa protein phosphatase 1a (OsPP1a) involved in salt stress tolerance in transgenic rice, Molecular Breeding, 2016, 36, 22 (ISI, IF: 2.465).
5 Chih-Ming Chiang, Chiu-Chen Chen, Shi-Peng Chen, Kuan-Hung Lin, Li-Ru Chen, Yu-Huei Su, His-Cheng Yen, Overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic Arabidopsis, Journal of Plant Research, 2017, 130(2), 373–386 (ISI, IF: 1.899).



Publication 2016-2017
No Authors
1 Thanh-Dao Tran, Thi-Cam-Vi Nguyen, Ngoc-Son Nguyen, Dai-Minh Nguyen, Thi-Thu-Ha Nguyen, Minh-Tri Le, Khac-Minh Thai, Synthesis of Novel Chalcones as Acetylcholinesterase Inhibitors, Appl. Sci. 2016, 6(7), 198 (Impact Factor: 1.726).
2 Song Thi Le, Chisa Yasuoka, Haruyasu Asahara and Nagatoshi Nishiwaki, Dual Behavior of Iodine Species in Condensation of Anilines and Vinyl Ethers Affording 2-Methylquinolines. Molecules, 2016, 21, 827 (Impact Factor: 2.465). 
3 Song Thi Le,  Toshiki Fujimoto, Haruyasu Asahara  and   Nagatoshi Nishiwaki,    Synthesis of 6-substituted 2-phenacylpyridines from 2-(phenylethynyl)pyridine via isoxazolo[2,3-a]pyridinium salt, Org. Biomol. Chem., 2016, 14, 10674 – 10682 (Impact Factor: 2.465).
4 Cédric Tarayre, Huu-Thanh Nguyen, Alison Brognaux, Anissa Delepierre, Lies De Clercq, Raphaëlle Charlier, Evi Michels, Erik Meers and Frank Delvigne, Characterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review, Sensors 2016, 16, 797 (Impact Factor: 2.033).
5 Lo Nhat Truong, Marc Dussauze, Mathieu Allix, Sébastien Chenu, Thierry Cardinal, Evelyne Fargin, Luís F. Santos, Second harmonic generation in germanotellurite bulk glassceramics, J. Am. Ceram. Soc. 2017, 1–12 (Impact Factor: 2.787). 
6 Zane Zondaka, Arko Kesküla, Tarmo Tamm, Rudolf Kiefer, Polypyrrole linear actuation tuned by phosphotungstic acid, Sensors and Actuators B: Chemical, 2017, 247, 742-748 (Impact Factor: 4.758).
7 Rudolf Kiefer,  Arko Kesküla, Jose G. Martinez, Gholamreza Anbarjafari, Janno Torop, Toribio F. Otero, Interpenetrated triple polymeric layer as electrochemomechanical actuator: Solvent influence and diffusion coefficient of counterions, Electrochimica Acta, 230, 2017, 461 – 469 (Impact Factor: 1.58).
8 Saber Imani, Chunli Wei, Jingliang Cheng, Md. Asaduzzaman Khan, Shangyi Fu, Luquan Yang, Mousumi Tania, Xianqin Zhang, Xiuli Xiao, Xianning Zhang, Junjiang Fu, MicroRNA-34a targets epithelial to mesenchymal transition-inducing transcription factors (EMT-TFs) and inhibits breast cancer cell migration and invasion, Oncotarget, 2017, 8 (13), 21362 – 21379 (Impact Factor: 5.008).
9 Md. Asaduzzaman Khan, Mousumi Tania, Shangyi Fu, Junjiang Fu, Thymoquinone, as an anticancer molecule: from basic research to clinical investigation, Oncotarget, 2017, doi: 10.18632/oncotarget.17206 (Impact Factor: 5.0). 
10 Jingliang Cheng, Shangyi Fu, ChunliWei, Mousumi Tania, Md. Asaduzzaman Khan, Saber Imania, Baixu Zhou, Hanchun Chen, Xiuli Xiao, JingboWug and Junjiang Fu, Evaluation of PIK3CA mutations as a biomarker in Chinese breast carcinomas from Western China, Cancer Biomark., 2017;19(1):85-92 (Impact Factor: 1.74). 
11 Khon C. Huynh, Thi-Hiep Nguyen, Dinh Chuong Pham, Huong T.T. Nguyen, Toi Van Vo, Marianna Gyenes, Volker R. Stoldt, Integrin αIIbβ3-Dependent ERK Signaling Is Regulated by Src and Rho Kinases in Both Leu33 and Pro33 Polymorphic Isoforms, Acta Haematol 2017, 137, 44–50 (Impact Factor: 1.053).
12 Phung Ngan Le, Dinh Chuong Pham, Dai Hai Nguyen, Ngoc Quyen Tran, Vladimir Dimitrov, Petko Ivanov, Cuong Nguyen Xuan, Hoai Nam Nguyen and Cuu Khoa Nguyen, Poly (N-isopropylacrylamide)-functionalized dendrimer as a thermosensitive nanoplatform for delivering malloapelta B against HepG2 cancer cell proliferation, Advances in Natural Sciences: Nanoscience and Nanotechnology, 2017, 8(2), 025014  (Impact Factor: 1.58).
13 Hoang Chinh Nguyen, Thi Van Anh TRAN, Quoc Linh NGUYEN, Nhu Nhut NGUYEN, Minh Khiem NGUYEN, Ngoc Thanh Tam NGUYEN, Chia-Hung SU, Kuan-Hung LIN, Newly Isolated Paecilomyces lilacinus and Paecilomyces javanicus as Novel Biocontrol Agents for Plutella xylostella and Spodoptera litura, Not Bot Horti Agrobo, 2017, 45(1), 280 – 286 (Impact Factor: 0.48).
14 Hoang Chinh Nguyen, Shih-Hsiang Liang, Thien Thanh Doan, Chia-Hung Su, Pei-Cheng Yang, Lipase-catalyzed synthesis of biodiesel from black soldier fly (Hermetica illucens): Optimization by using response surface methodology, Energy Conversion and Management, 2017, 145, 335-342 (Impact Factor: 5.589). 
15 Thien-KhanhTran, Kuo-FengChiu, Chiu-YueLin, Hoang-JyhLeu, Electrochemical treatment of wastewater: selectivity of the heavy metals removal process, International journal of hydrogen energy, 2017, (Impact Factor: 3.313).
16 Bharath Kumar Velmurugan, Chien-Hung Lee, Shang-Lun Chiang, Chun-Hung Hua, Mei-Chung Chen, Shu-Hui Lin, Kun-Tu Yeh, Ying-Chin Ko, PP2A deactivation is a common event in oral cancer and reactivation by FTY720 shows promising therapeutic potential, J Cell Physiol.2017, doi: 10.1002/jcp.26001 (Impact Factor: 3.839).
17 Pai-An Hwang, Ming-De Yan, Ko-Liang Kuo, Nam Nhut Phan, Yen-Chang Lin, A mechanism of low molecular weight fucoidans degraded by enzymatic and acidic hydrolysis for the prevention of UVB damage, Journal of Applied Phycology, 29 (1), 521-529 (Impact Factor: 2.616).
18 Nam Nhut Phan, Tung Thanh Huynh, Yen-Chang Lin, Hyperpolarization-Activated Cyclic Nucleotide-Gated Gene Signatures and Poor Clinical Outcome of Cancer Patient, Translational Cancer Research; accepted manuscript, 2017 (Impact Factor: 1.167).
19 Do Thi Duyen, Nam Nhut Phan, Chih-Yang Wang, Zhengda Sun, Yen-Chang Lin, Novel regulations of MEF2-A, MEF2-D, and CACNA1S in the functional incompetence of adipose-derived mesenchymal stem cells by induced indoxyl sulfate in chronic kidney disease, Cytotechnology, 68 (6), 2589-2604(Impact Factor: 1.857).
20 Thuy TD, Phan NN, Wang CY, Yu HG, Wang SY, Huang PL, Do YY, Lin YC, Novel therapeutic effects of sesamin on diabetes-induced cardiac dysfunction, Mol Med Rep. 2017, 15(5), 2949-2956 (Impact Factor: 1.60).
21 Pai-An Hwang, Nam Nhut Phan, Wen-Jung Lu, Bui Thi Ngoc Hieu, Yen-Chang Lin, Low-molecular-weight fucoidan and high-stability fucoxanthin from brown seaweed exert prebiotics and anti-inflammatory activities in Caco-2 cells, Food and nutrition research, 2016, 60, 32033(Impact Factor: 1.676).
1 Chih‐Yang Wang, Payam Shahi, John Ting Wei Huang, Nam Nhut Phan, Zhengda Sun, Yen‐Chang Lin, Ming‐Derg Lai, Zena Werb, Systematic analysis of the achaete‐scute complex‐like gene signature in clinical cancer patients, Molecular And Clinical Oncology, 2017, 6(1), 7–18. 
2 Sharanjit Singh, Nguyen Thi Anh Nga, Thong L.M. Pham, Tan Ji Siang, Pham T. T. Phuong, Maksudur R. Khan, Dai-Viet N. Vo, Metgas Production from Bi-reforming of Methane over Lamodified Santa Barbara Amorphous-15 Supported Nickel Catalyst, Chemical Engineering Transactions,  56, 1573-1578

Publication 2015 - 2016

No Authors
1 Thanh-Dao Tran, Thi-Cam-Vi Nguyen, Ngoc-Son Nguyen, Dai-Minh Nguyen, Thi-Thu-Ha Nguyen, Minh-Tri Le, Khac-Minh Thai (2016) Synthesis of Novel Chalcones as Acetylcholinesterase Inhibitors, Appl. Sci., 6(7), 198; doi:10.3390/app6070198 (Impact factor: 1.726)
2 Nguyen, H.T., Truong, D.H., Kouhounde, S., Ly, S., Razafindralambo, H., Delvigne, F. (2016) Biochemical Engineering Approaches for Increasing Viability and Functionality of Probiotic Bacteria. Int J Mol Sci, 17(6). Doi:10.3390/ijms17060867 (Impact Factor: 3.257,H index: 64)
3 Do, T.-H., Nguyen, D.M., Truong, V.D., Do, T.H., Le, M.T., Pham, T.Q., Thai, K.M., Tran, T.D. (2016) Synthesis and Selective Cytotoxic Activities on Rhabdomyosarcoma and Noncancerous Cells of Some Heterocyclic Chalcones. Molecules, 21(3). Doi: 10.3390/molecules21030329 (Impact Factor: 2.465, H index: 81)
4 Nguyen, M.-H., Nguyen, T.-H.-N., Hwang, I.-C., Bui, C.-B., Park, H.-J. (2016) Effects of the physical state of nanocarriers on their penetration into the root and upward transportation to the stem of soybean plants using confocal laser scanning microscopy. Crop Protection, 87, 25-30 (Impact Factor: 1.493, H index: 70).
5 Nguyen, M.-H., Tran, T.T., Hadinoto, K. (2016) Controlling the burst release of amorphous drug-polysaccharide nanoparticle complex via crosslinking of the polysaccharide chains. Eur J Pharm Biopharm, 104, 156-163. (Impact Factor: 4.245, H index: 114).
6 Yung C. Lai, Shu Y. Wang, Huan Y. Gao, Khiem M. Nguyen, Chinh H. Nguyen, Ming C. Shih, Kuan H. Lin (2016) Physicochemical properties of starches and expression and activity of starch biosynthesis-related genes in sweet potatoes. Food Chem, 11,199:556-64. Doi:10.1016/j.foodchem.2015.12.053. (Impact Factor: 3.391, H index: 169 )
7 C.W. Wu, M.C. Lee, Y.L. Peng, T.Y. Chou, K.H.Lin, and Y.S. Chang. (2016) Chlorophyll fluorescence upper-to-lower-leaf ratio for determination of irrigation time for Pentas lanceolate. Photosynthetica, 54 (2): 193-200. DOI: 10.1007/s11099-015-0175-5(Impact Factor: 1.409, H index: 47 )
8 Ngoc Lan Mai, Chan Kyung Kim, Byungho Park, Heon-Jin Park, Sang Huyn Lee, Yoon-Mo Koo. (2016) Prediction of cellulose dissolution in ionic liquids using molecular descriptors based QSAR model. Journal of Molecular Liquids 215, 541-548. Doi:10.1016/j.molliq.2016.01.040 (Impact Factor: 2.515, H index: 52)
9 Ngoc Lan Mai, and Yoon-Mo Koo (2015) Computer-aided design of ionic liquids for high cellulose dissolution. ACS Sustainable Chemistry & Engineering 4 (2), 541-547. DOI: 10.1021/acssuschemeng.5b00958 (Impact Factor: 5.267, H index: 24)
10 Sang-Woo Bae, Doyoung E om, Ngoc Lan Mai and Yoon-Moo Koo. (2016) Refolding of horseradish per oxidase is enhanced in presence of metal cofactors and ionic liquids. Biotechnology Journal 11(4):464-472. (Impact Factor: 3.49, H index: 51)
11 Qing Xie, Kang-Ning Shen, Xiuying Hao, Phan Nhut Nam, Bui Thi Ngoc Hieu, Ching-Hung Chen, Changqing Zhu, Yen-Chang Lin & Chung-Der Hsiao. (2015) The complete chloroplast genome of Tianshan Snow Lotus (Saussurea involucrata), a famous traditional Chinese medicinal plant of the family Asteraceae. Mitochondrial DNA Doi: 10.3109/19401736.2015.1118086 (Impact Factor: 1.209, H index: 25)
12 Nam Nhut Phan, Chih-Yang Wang, Chien-Fu Chen, Zhengda Sun, Ming-Derg Lai, Yen-Chang Lin. (2016) Voltage-gated calcium channels: novel targets for cancer therapy, Oncology Letters (minor revision, consider for publication) (Impact Factor: 1.554, H index:20)
13 Truong, D.H., Bauwens, J., Delaplace, P., Mazzucchelli, G., Lognay, G., Francis, F. (2015) Proteomic analysis of Arabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer). Plant Biology, 17(6), 1210-1217. DOI: 10.1111/plb.12363(Impact Factor: 2.216, H index: 63)
14 Nguyen, H.T., Razafindralambo, H., Richel, A., Jacquet, N., Evrard, P., Antoine, P., Thonart, P., Delvigne, F. (2015) Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria. Journal of Industrial Microbiology & Biotechnology, 42(9), 1225-1231. Doi:10.1007/s10295-015-1650-5 (Impact Factor: 2.732,H index: 80).
15 Truong DH (2015). Variation in volatile profiles of Arabidopsis thaliana Col-0 Heynh plants due to herbivorous infestation: piercing-sucking versus chewing insects. Proceedings of 2015 international Conference on Biologl abd Environmental Sciences (BIOES-15), Phuket,Thailand, October 1-2, 2015.


Publication 2014 - 2015

No Authors
1 Dan, Q.T and Nam Nhut Phan.2015. Optimization of supercritical C02 extraction of oleoresin from black pepper ( Piper nigrum L.) and antioxidant capacity of the oleoresin. International Food research journal, p1489-1493.
2 Chih-Yang Wang, Ming-Derg Lai, Nam Nhut Phan, Zhengda Sun, Yen – Chang Lin. 2015. Meta-analysis of public microarray datasets reveals voltage-gated calcium gene signatures in clinical cancer patients. Plos One, p32
3 Pai-An Hwang, Yu-Lan Hung, Nam Nhut Phan, Hieu Thi Ngoc Bui, Po-Ming Chang, Luan-Lun Li, Yen-Chang Lin. 2015. Cytotechnology, p11

Publication before 2014

No Authors
1 Duong Tan Nhut, Tran Ngoc Thuy Tien, Mai Thi Ngoc Huong, Nguyen Thi Thanh Hien, Phan Xuan Huyen, Bui Van Le and Do Nang Vinh. 2004. Some results in study on artificial seeds of Lilium spp. Vietnamese Academy of Science and Technology, Journal of Biotechnology. Vol. 2 (3): 359 – 370.
2 Nguyen Thi Thanh Hien, Dinh Van Khiem, Nguyen Hong Vu, Nguyen Trinh Don and Duong Tan Nhut. 2004. Primary study on the induction and growth of callus of Taxus wallichiana Zucc., a valuable medicinal plant in Lam Dong Province. Journal of Agricultural Sciences and Technology, Nong Lam University. No 4: 79 – 85.
3 Nguyen Thi Thanh Hien, Dinh Van Khiem, Nguyen Hong Vu, Nguyen Trinh Don and Duong Tan Nhut. 2005. Primary study on the induction and growth of callus of Taxus wallichiana Zucc., a valuable medicinal plant in Lam Dong Province. Proceedings of Vietnam-Korea International Symposium on Biotechnology and Bio-system engineering. pp, 191 - 197.
4 Duong Tan Nhut, Tran Ngoc Thuy Tien, Mai Thi Ngoc Huong, Nguyen Thi Thanh Hien, Phan Xuan Huyen, Vu Quoc Luan and Jaime A. Teixeira da Silva. 2005. Artificial seeds for propagation and preservation of Cymbidium spp. Propagation of Ornamental Plants. Vol. 5 (2): 67 - 73.
5 Suriyan Cha-um, Nguyen Thi Thanh Hien, and Chalermpol Kirdmanee. 2006. Desease-free production of Sugarcane varieties (Saccharum officinarum L.) using in vitro meristem culture. Biotechnology Vol. 5 (4): 433 – 448.
6 Duong Tan Nhut, Nguyen Thi Thanh Hien, Nguyen Trinh Don and Dinh Van Khiem. 2007. In vitro shoot development of Taxus wallichiana Zucc., a valuable medicinal plant. In: Mohan Jain S. and Häggman H. (eds), Protocols for Micropropagation of Woods Trees and Fruits, Springer, The Netherlands. pp, 107 – 116.
7 Duong Tan Nhut, Nguyen Trinh Don, Nguyen Thi Thanh Hien, Dinh Van Khiem, Phan Xuan Huyen. 2007. Cell culture and callus recovery from suspension of Hymalayan Yew (Taxus wallichiana Zucc.). Vietnamese Academy of Science and Technology, Journal of Biotechnology Vol. 5 (2): 243 – 253.
8 Ngoc Nhiem Dao, Minh Dai Luu, Quang Khuyen Nguyen and Byung Sun Kim, UV absorption by cerium oxide nanoparticles/epoxy composite thin films, Advances in Natural Sciences: Nanoscience and Nanotechnology, 2 (2011), 045013.
9 Do Tuong Ha, Tran Thanh Dao. Synthesis of some flavones and thioflavones as potential anti-bacterial agents. Science and technology development Journal. The twelfth year. Volume 12 – No 03/2009, p-33-39
10 Tran Phi Hoang Yen, Nguyen Bao Uyen, Vo Phung Nguyen, Đo Tuong Ha, Tran Thanh Dao*. Validation of in-vivo anti-oxidative activity of some flavone semi-synthesized from rutin. Ho Chi Minh City Medical Research, Vol.13, Supp 1, p-157 (01-2009)
11 Tran Le Anh Thuy, Nguyen Thi Thao Nhu, Do Tuong Ha, Vo Thi Anh Thu, Tran Thanh Dao. Synthesis and evaluation of anti-microbial activity of some heterocyclic chalcones. Y hoc Tp.HCM Vol.14 – Supplement of No 1 – 2010 : p.110-115
12 Nguyen Thi Thu Giang, Do Tuong Ha, Khac Minh Thai, Vo Phung Nguyen, Tran Thanh Dao. Synthesis and evalution of anti-inflammatory activity of some Polyoxychalcone. Y hoc Tp.HCM Vol.14 – Supplement of No 1 – 2010 : p-93-99
13 T. Ha Do; T. Thao-Nhu Nguyen; T. Anh-Thu Vo; Thanh-Dao Tran. Synthesis and antibacterial activity against Staphylococus aureus of some heterocyclic chalcone derivatives. Journal of Chemistry, Vol.48 (4B), p. 13 - 18, 11/2010
14 Tran Thi Kim Thoa, Do Tuong Ha, Nguyen Thi Cam Vi, Tran Cat Dong, Tran Thanh Dao. Antimicrobial action of some flavonoids, separate and in combination on the methicillin-resistant Staphycolocus aureus. Pharmaceutical Journal, No 417, Vol.51, p.24-28 (01-2011)
15 Thanh-Dao Tran, Tuong-Ha Do, Ngoc-Chau Tran, Trieu-Du Ngo, Thi-Ngoc-Phuong Huynh, Cat-Dong Tran, and Khac-Minh Thai. Synthesis and anti Methicillin Resistant Staphylococcus aureus activity of substituted chalcones alone and in combination with non-beta-lactam antibiotics. Bioorganic & Medicinal Chemistry Letters, 2012, Vol. 22, in press doi: 10.1016/j.bmcl.2012.05.112
16 Thanh-Dao Tran, Thi-Thao-Nhu Nguyen, Tuong-Ha Do, Thi- Ngoc-Phuong Huynh, Cat-Dong Tran and Khac-Minh Thai. Synthesis and Antibacterial Activity of Some Heterocyclic Chalcone Analogues Alone and in Combination with Antibiotics. Molecules 2012, 17(6), 6684-6696
17 Vo Thanh Phong, Nguyen Thi Thu Ha, Do Tuong Ha, Truong Ngoc Tuyen, Tran Thanh Dao. Synthesis of Nicorandil. Pharmaceutical Journal Vol.18 – Supplement of No 2 – 2014: 391 – 394.
18 Trinh Thi Lan Anh, Ho Thanh Tam, Le Kim Cuong, Nguyen Ba Nam, Nguyen Thi Thanh Hien, Vo Thi Bach Mai, Duong Tan Nhut. Optimization Palaenopsis amabilis from protoplast in breeding. Biotechnology magazine 11 (4): 705 -716, 2013.
19 Nguyen Thi Thu Ha, Truong Ngoc Tuyen, Le Minh Tri, Tran Boi Chau, Tran Thanh Dao. Optimization of parameter for procedure of synthesis of Felodipine. Pharmaceutical Journal Vol 18 – Supplement of No 2-2014: 381-385
20 Truong DH, Bauwens J, Delaplace P, Mazzucchelli G, Lognay G, Francis F. (2015). Proteomic analysis ofArabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer). Plant Biology, 17: 1210-1217.
21 Truong DH (2015). Variation in volatile profiles of Arabidopsis thaliana Col-0 Heynh plants due to herbivorous infestation: piercing-sucking versus chewing insects. Proceedings of 2015 international Conference on Biologl abd Environmental Sciences (BIOES-15), Phuket,Thailand, October 1-2, 2015.
22 Truong DH, Bauwens J, Delaplace P, Mazzucchelli G, Lognay G, Francis F. (2015). Proteomic analysis of Arabidopsis thaliana (L.) Heynh responses to a generalist sucking pest (Myzus persicae Sulzer). Plant Biology, 17: 1210-1217.
23 Nguyen, H-T, Razafindralambo, H., Richel, A., Jacquet, N., Evrard, P., Antoine, P., Thonart, P. and Delvigne, F., 2015. Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria. Journal of Industrial Microbiology & Biotechnology: 1-7. DOI: 10.1007/s10295-015-1650-5
24 Mai, N.L. and Y.-M. Koo, Computer-aided design of ionic liquids for high cellulose dissolution. ACS Sustainable Chemistry & Engineering, 2015.